In:
International Journal for Vitamin and Nutrition Research, Hogrefe Publishing Group, Vol. 90, No. 3-4 ( 2020-06), p. 239-248
Abstract:
Abstract. Folate could have an antioxidant role but also may be detrimental under vitamin B 12 deficiency. The aim was to investigate the effect of different dietary folic acid (FA) levels, on oxidative stress in B 12 induced deficient aged rats. Thirty-five male aged Sprague–Dawley rats, were fed either a vitamin B 12 deficient (n = 27) or a control diet (n = 8) during eight weeks. Then, animals were divided into four groups: B 12 and FA deficient diet (DBDF), B 12 deficient diet and FA control diet (DBCF), B 12 deficient diet and FA supplemented diet (DBSF), and control diet (CBCF) for a 30 days period. Methionine metabolism and antioxidant status were evaluated. Both vitamins deficiencies elevated serum homocysteine (Hcy) (7.7 vs. 4.3 μmol/L, p 〈 0.05) and reduced S-adenosylmethionine hepatic content (283.7 vs. 581.9 μg/g protein, p 〈 0.05), the total antioxidant capacity (155.7 vs. 189.3 μmol/L, p 〈 0.05), glutathione (GSH) (120.5 vs. 419.9 μg/mg protein, p 〈 0.05) and oxidized glutathione (0.9 vs. 2.6 μg/mg protein, p 〈 0.05) compared to control. Activities of glutathione peroxidase and glutathione reductase enzymes or damage to macromolecules were unaffected. Adequate or elevated dietary FA in B 12 deficiency rats decreased Hcy (5.7 and 6.3 μmol/L, respectively) and increased total antioxidant capacity (189.8 and 192.6 μmol/L, respectively) to values similar to control group, whereas GSH concentration was significantly lower than control (209.1 and 208.0 μg/mg protein respectively, p 〈 0.05). In conclusion, in a vitamin B 12 deficiency status, adequate or elevated FA prevented impairment in one-carbon metabolism, but does not fully reverse the decrease in antioxidant capacity.
Type of Medium:
Online Resource
ISSN:
0300-9831
,
1664-2821
DOI:
10.1024/0300-9831/a000537
Language:
English
Publisher:
Hogrefe Publishing Group
Publication Date:
2020
SSG:
15,3
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