In:
Alimentary Pharmacology & Therapeutics, Wiley, Vol. 16, No. s2 ( 2002-04), p. 137-144
Abstract:
RT–PCR amplification of tumour‐specific mRNA has been used for the detection of cancer cells in peripheral blood. Aim: To evaluate the characteristics of the tumour specific mRNA species in peripheral blood of stomach cancer patients. Methods: We analysed CEA, GalNAc‐T, MUC‐1, c‐MET and hTERT mRNA expression in the stomach cancer cell lines and tissues, lymph nodes and peripheral blood of stomach cancer patients using RT–PCR. Results: In RT–PCR analysis of the peripheral blood, 4%, 8%, 21%, 46%, and 100% of stomach cancer patients were positive for CEA, GalNAc‐T, c‐MET, hTERT and MUC‐1 mRNA, respectively, but MUC‐1 mRNA was also positive in all normal blood samples. The detection of hTERT mRNA was correlated with poor differentiation ( P =0.01) and lymph node metastasis ( P =0.009). The presence of c‐MET mRNA was correlated with T stage ( P =0.025), lymph node metastasis ( P =0.036), distant metastasis ( P =0.031), and stage of the stomach cancer ( P =0.023). Conclusions: Our study suggest that hTERT mRNA in peripheral blood can be a molecular marker for gastric cancer. We also showed that each molecular marker can be correlated with the clinicopathological features of the patients.
Type of Medium:
Online Resource
ISSN:
0269-2813
,
1365-2036
DOI:
10.1046/j.1365-2036.16.s2.33.x
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2003094-0
SSG:
15,3
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