In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 57, No. 4 ( 2010-02-18), p. 475-481
Abstract:
Peroxynitrite (ONOO−), a potent cytotoxic oxidant formed by the reaction of nitric oxide (*NO) and superoxide radical (*O2−), may be rapidly lethal in a cellular milieu due to oxidization and nitration processes. In the present study, hydroquinone displayed strong ONOO− scavenging activity and inhibitory effect on NO production in murine macrophage RAW264.7 cells. Hydroquinone strongly scavenged ONOO− induced dihydrorhodamine 123 oxidation in a dose-dependent manner compared with other reactive species such as *O2− and *NO. Hydroquinone also decreased levels of ONOO− induced nitrotyrosine of glutathione reductase and consequently prevented the enzyme from ONOO− induced damage. Furthermore, hydroquinone suppressed NO production, a cellular pathway for ONOO− formation, in lipopolysaccharide-activated RAW264.7 cells via inhibition of inducible NO synthase expression. The inhibitory effect by hydroquinone seems to be mediated by interruption of lipopolysaccharide-induced signalling such as activation of nuclear factor-kB and extracellular signal-related kinases 1 and 2. The results suggest that hydroquinone may potently modulate reactivity of ONOO− and may therefore be a useful agent against ONOO− mediated diseases.
Type of Medium:
Online Resource
ISSN:
0022-3573
,
2042-7158
DOI:
10.1211/0022357055731
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2010
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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