In:
Phytotherapy Research, Wiley, Vol. 28, No. 2 ( 2014-02), p. 274-279
Abstract:
We isolated crystals from the chloroform fraction of an ethanol extract of Kaempferia galanga and identified it as ethyl p ‐methoxycinnamate through nuclear magnetic resonance analysis. In the present study, we found that ethyl p ‐methoxycinnamate significantly decreased melanin synthesis in B16F10 murine melanoma cells stimulated with α‐melanocyte stimulating hormone (α‐MSH). In a cell‐free system, however, ethyl p ‐methoxycinnamate did not directly inhibit tyrosinase, the rate‐limiting enzyme of melanogenesis. Instead, it inhibited tyrosinase activity in B16F10 cells in a dose‐dependent manner. Furthermore, Western blot analysis showed that ethyl p ‐methoxycinnamate decreased microphthalmia‐associated transcription factor and tyrosinase levels in α‐MSH‐stimulated B16F10 cells. These results indicate that the pigment‐inhibitory effect of ethyl p ‐methoxycinnamate results from downregulation of tyrosinase. Ethyl p ‐methoxycinnamate isolated from K. galanga could be developed as a skin whitening agent to treat hyperpigmentary disorders. Copyright © 2013 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0951-418X
,
1099-1573
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
1493490-5
SSG:
15,3
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