In:
Nanomedicine, Future Medicine Ltd, Vol. 13, No. 12 ( 2018-06), p. 1417-1432
Abstract:
Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH) 2 D 3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH) 2 D 3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH) 2 D 3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH) 2 D 3 -loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH) 2 D 3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.
Type of Medium:
Online Resource
ISSN:
1743-5889
,
1748-6963
DOI:
10.2217/nnm-2018-0011
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2018
SSG:
15,3
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