In:
Natural Product Communications, SAGE Publications, Vol. 7, No. 9 ( 2012-09), p. 1934578X1200700-
Kurzfassung:
A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R 2 〉 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.
Materialart:
Online-Ressource
ISSN:
1934-578X
,
1555-9475
DOI:
10.1177/1934578X1200700920
Sprache:
Englisch
Verlag:
SAGE Publications
Publikationsdatum:
2012
ZDB Id:
2430442-6
SSG:
15,3
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