In:
Bioanalysis, Future Science Ltd, Vol. 3, No. 11 ( 2011-06), p. 1271-1285
Abstract:
Neuropeptides seem to play an important role when the CNS is challenged. In order to obtain better insights into the central peptidergic effects, it is essential to monitor their concentration in the brain. Quantification of neuropeptides in dialysates is challenging due to their low extracellular concentrations (low pM range), their low microdialysis efficiencies, the need for acceptable temporal resolution, the small sample volumes, the complexity of the matrix and the tendency of peptides to stick to glass and polymeric materials. The quantification of neuropeptides in dialysates therefore necessitates the use of very sensitive nano-LC–MS/MS methods. A number of LC–MS/MS and microdialysis parameters need to be optimized to achieve maximal sensitivity. The optimized and validated methods can be used to investigate the in vivo neuropeptide release during pathological conditions, in this way initiating new and immense challenges for the development of new drugs.
Type of Medium:
Online Resource
ISSN:
1757-6180
,
1757-6199
Language:
English
Publisher:
Future Science Ltd
Publication Date:
2011
SSG:
15,3
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