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  • 1
    In: Science, American Association for the Advancement of Science (AAAS), Vol. 379, No. 6634 ( 2023-02-24)
    Abstract: Observations of asteroid Ryugu by the Hayabusa2 spacecraft found that it is a rubble pile, formed from fragments of a parent asteroid. Samples retrieved from Ryugu by the spacecraft were expected to contain a record of this history, including the formation and early evolution of the parent body, the subsequent impact destruction and partial reaccretion, and later space weathering. The composition of Ryugu was expected to be similar to that of Ivuna-type carbonaceous chondrite meteorites (CI chondrites). RATIONALE We investigated the formation history of Ryugu through laboratory analysis of the samples. Specifically, we sought to determine (i) when and where in the Solar System the parent asteroid formed; (ii) the original mineralogy, elemental abundances as a whole, and chemical compositions of the accreted materials, including their ice content; (iii) how these materials evolved through chemical reactions; and (iv) how the material was ejected from the parent body in an impact. To address these issues, we analyzed 17 Ryugu particles of 1 to ~8 mm in size. RESULTS We found carbon dioxide (CO 2 )–bearing water in an iron-nickel (Fe–Ni) sulfide crystal, indicating that the parent body formed in the outer Solar System. Remanent magnetization was detected, implying that the solar nebula might still have been present when magnetite crystals formed on the parent body. We used muon analysis to determine the abundances of light elements, including carbon (C), nitrogen (N), sodium (Na), and magnesium (Mg), whose abundances relative to silicon (Si) are similar to those in CI chondrites, whereas oxygen (O) is deficient compared with that in CI chondrites. X-ray computed tomography analysis shows that all our Ryugu samples consist of fine-grained material. There are only rare objects of high-temperature origin, such as melted silicate-rich particles, all being smaller than 100 μm. Electron microscope observations showed that the samples are breccias, assemblies of numerous small rock fragments with different compositions, mineralogies, and histories. The most common mineralogy contains Mg-rich hydrous silicates, MgCa and MgFe carbonates, hydroxyapatite, Fe sulfides, and Fe oxides. The mineralogy of this major lithology is consistent with classification as a CI chondrite. It also indicates widespread aqueous alteration (reactions with liquid water) on the parent body. Some Ryugu fragments have a different mineralogy, containing anhydrous silicates (olivine and pyroxene), amorphous silicates, Ca carbonate, phosphides, Fe–Ni sulfide, Fe oxide, and poorly crystalline phyllosilicates. Some small objects ( 〈 ~30 μm) that formed at high temperatures were also found. This mineralogy suggests that these fragments experienced less aqueous alteration. We measured mechanical and thermal properties from the Ryugu samples. We found that they are similar, but not identical, to hydrated CI chondrites. Numerical simulations of the thermal history and impact disruption processes of the Ryugu parent asteroid were performed by incorporating the physical and mineralogical properties and appropriate water/rock ratios. CONCLUSION We propose that Ryugu’s parent asteroid formed ~1.8 million to 2.9 million years after the beginning of Solar System formation, in the outer Solar System, where water and CO 2 were present as ice. It acquired a water ice/rock mass ratio in the range of 0.2 to 0.9. In this region, material formed at low temperatures is dominant, whereas material of high temperature origin is rare. In the interior of the parent asteroid, radioactive heating caused the water ice to melt at ~3 million years; water-rock reactions then gradually changed the initial anhydrous mineralogy to a largely hydrous mineralogy. At shallow depths, the original material was less altered, at a low water/rock ratio of 〈 0.2. At ~5 million years, all material in the parent asteroid experienced its peak temperature, and aqueous alteration continued. An impact occurred ~1 billion years ago, disrupting the parent asteroid. Some fragments, originating away from the impact point, then reassembled to form Ryugu. Proposed model of Ryugu’s formation history. (1) A parent body asteroid forms in the outer Solar System, containing abundant ice. (2 and 3) Radioactive heating causes the ice to melt, which modifies the mineralogy through aqueous alteration reactions. (4 and 5) An impact then disrupts the parent body but does not cause widespread heating. (6) Ryugu formed from reaccumulation of ejected material that originated away from the impact point. All times were measured from the start of Solar System formation. Colors indicate estimated temperatures from our thermal interior and impact models.
    Type of Medium: Online Resource
    ISSN: 0036-8075 , 1095-9203
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    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2023
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  • 2
    Online Resource
    Online Resource
    IOP Publishing ; 1987
    In:  Europhysics Letters (EPL) Vol. 3, No. 1 ( 1987-01-01), p. 39-44
    In: Europhysics Letters (EPL), IOP Publishing, Vol. 3, No. 1 ( 1987-01-01), p. 39-44
    Type of Medium: Online Resource
    ISSN: 0295-5075 , 1286-4854
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 1987
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    detail.hit.zdb_id: 165776-8
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  • 3
    In: Science, American Association for the Advancement of Science (AAAS), Vol. 366, No. 6464 ( 2019-10-25), p. 480-485
    Abstract: Soil organisms, including earthworms, are a key component of terrestrial ecosystems. However, little is known about their diversity, their distribution, and the threats affecting them. We compiled a global dataset of sampled earthworm communities from 9212 sites in 57 countries as a basis for predicting patterns in earthworm diversity, abundance, and biomass. We found that local species richness and abundance typically peaked at mid-latitudes, displaying patterns opposite to those observed in aboveground organisms. However, high species dissimilarity across tropical locations may cause diversity across the entirety of the tropics to be higher than elsewhere. Climate variables and habitat cover were found to be more important in shaping earthworm communities than soil properties. These findings suggest that climate and habitat change may have serious implications for earthworm communities and for the functions they provide.
    Type of Medium: Online Resource
    ISSN: 0036-8075 , 1095-9203
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    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2019
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  • 4
    In: Journal of Neuroscience, Society for Neuroscience, Vol. 27, No. 23 ( 2007-06-06), p. 6261-6267
    Type of Medium: Online Resource
    ISSN: 0270-6474 , 1529-2401
    Language: English
    Publisher: Society for Neuroscience
    Publication Date: 2007
    detail.hit.zdb_id: 1475274-8
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  • 5
    Online Resource
    Online Resource
    Society for Neuroscience ; 1987
    In:  The Journal of Neuroscience Vol. 7, No. 5 ( 1987-05-01), p. 1520-1525
    In: The Journal of Neuroscience, Society for Neuroscience, Vol. 7, No. 5 ( 1987-05-01), p. 1520-1525
    Abstract: Three monoclonal antibodies were prepared against rat brain soluble protein kinase C. Two of the antibodies, CKI-97 (IgG2b subclass) and CKII-90 (IgG1 subclass), showed weak binding to native protein kinase C. The third antibody, CKI-33 (IgG2b subclass), showed no binding. However, the mixture of CKI-97, CKII-90, and CKI-33 exhibited much stronger binding activity to this protein kinase than any of the antibodies alone. Although none of these antibodies showed protein kinase C-neutralizing activity, Western blot analysis indicated that these antibodies reacted specifically with protein kinase C, presumably its subspecies, that is present predominantly in nervous tissues. Immunocytochemical studies shows that these antibodies can be used for identification of this enzyme in nervous tissues. In rat Purkinje cells, the immunoreactive material was present throughout the cytoplasm, including dendrites and axons, but was poorly represented in the cell nucleus. In cerebellum, the localization of protein kinase C appears to be very similar to that of cGMP-dependent protein kinase.
    Type of Medium: Online Resource
    ISSN: 0270-6474 , 1529-2401
    Language: English
    Publisher: Society for Neuroscience
    Publication Date: 1987
    detail.hit.zdb_id: 1475274-8
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  • 6
    Online Resource
    Online Resource
    Proceedings of the National Academy of Sciences ; 1981
    In:  Proceedings of the National Academy of Sciences Vol. 78, No. 12 ( 1981-12), p. 7674-7678
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 78, No. 12 ( 1981-12), p. 7674-7678
    Abstract: The immune response to phosphocholine in BALB/c mice involves one group of heavy chain variable region (VH) genes and at least three groups of light chain variable region (V kappa) genes, represented by the gene products of the myelomas TEPC 15, MOPC 603, and MOPC 167/MOPC 511. The amino acid sequences of BALB/c myeloma kappa chains MOPC 167 and MOPC 511 are known, and they differ by six amino acids. We have isolated several closely related V region genes of immunoglobulin light chains from a mouse sperm DNA phage library, selecting clones that cross-hybridize with a cDNA plasmid probe encoding the light chain of MOPC 167. We identified six strongly hybridizing clones, representing three separate cloning events. We determined the sequence of the coding and immediate flanking regions of three clones, representing the three separate cloning events, and they proved to be identical. This germ-line sequence encoded the amino acid sequence of neither MOPC 167 nor MOPC 511, but required four base pair changes to generate the V kappa M167 cDNA sequence and five base pair changes to generate the V kappa M511 gene. By Southern hybridization experiments, we demonstrated that neither MOPC 511 nor MOPC 167 germ-line genes exist. We conclude that the V kappa M167 and V kappa M511 genes are created somatically.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 1981
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  • 7
    Online Resource
    Online Resource
    Proceedings of the National Academy of Sciences ; 2011
    In:  Proceedings of the National Academy of Sciences Vol. 108, No. 38 ( 2011-09-20), p. 15721-15726
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 108, No. 38 ( 2011-09-20), p. 15721-15726
    Abstract: Mineralogical processes taking place close to equilibrium, or with very slow kinetics, are difficult to quantify precisely. The determination of ultraslow dissolution/precipitation rates would reveal characteristic timing associated with these processes that are important at geological scale. We have designed an advanced high-resolution white-beam phase-shift interferometry microscope to measure growth rates of crystals at very low supersaturation values. To test this technique, we have selected the giant gypsum crystals of Naica ore mines in Chihuahua, Mexico, a challenging subject in mineral formation. They are thought to form by a self-feeding mechanism driven by solution-mediated anhydrite-gypsum phase transition, and therefore they must be the result of an extremely slow crystallization process close to equilibrium. To calculate the formation time of these crystals we have measured the growth rates of the {010} face of gypsum growing from current Naica waters at different temperatures. The slowest measurable growth rate was found at 55 °C, 1.4 ± 0.2 × 10 -5  nm/s, the slowest directly measured normal growth rate for any crystal growth process. At higher temperatures, growth rates increase exponentially because of decreasing gypsum solubility and higher kinetic coefficient. At 50 °C neither growth nor dissolution was observed indicating that growth of giant crystals of gypsum occurred at Naica between 58 °C (gypsum/anhydrite transition temperature) and the current temperature of Naica waters, confirming formation temperatures determined from fluid inclusion studies. Our results demonstrate the usefulness of applying advanced optical techniques in laboratory experiments to gain a better understanding of crystal growth processes occurring at a geological timescale.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2011
    detail.hit.zdb_id: 209104-5
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  • 8
    Online Resource
    Online Resource
    Proceedings of the National Academy of Sciences ; 1997
    In:  Proceedings of the National Academy of Sciences Vol. 94, No. 12 ( 1997-06-10), p. 6279-6284
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 94, No. 12 ( 1997-06-10), p. 6279-6284
    Abstract: Interactions between the ureteric bud (UB) and metanephric mesenchyme are crucial for tubulogenesis during kidney development. Two immortalized cell lines derived from the day 11.5 embryonic kidney, UB cells, which appear to be epithelial (cytokeratin-positive, E-cadherin-positive, and ZO-1-positive by immunostaining) and BSN cells, which are largely mesenchymal (vimentin-positive, but negative for cytokeratin, cell surface E-cadherin, and cell surface ZO-1), were used to establish an in vitro tubulogenesis system. BSN cells expressed hepatocyte growth factor (HGF) and transforming growth factor-β1 mRNAs, and its conditioned medium (BSN-CM) contained factors capable of activating the epidermal growth factor (EGF) receptor (EGFR). When UB cells were cultured in an extracellular matrix gel in the presence of the embryonic kidney or BSN-CM, the UB cells underwent morphogenetic changes characteristic of early in vitro branching tubulogenesis. These changes were largely inhibited by a combination of neutralizing anti-HGF antibodies and the EGFR inhibitor tyrphostin AG1478, suggesting that EGFR ligands, together with HGF, account for much of this early morphogenetic activity. Nevertheless, there was a significant fraction of tubulogenic activity that could not be inhibited, suggesting the existence of other soluble factors. Whereas HGF, EGF, transforming growth factor α, basic fibroblast growth factor (bFGF), and insulin-like growth factor 1 (IGF-1), or a mixture of these growth factors, induced epithelial processes for up to 3 days, only IGF-1, possibly bFGF, and the mixture were able to sustain morphogenesis for longer periods, though not nearly to the same degree as BSN-CM. Moreover, only BSN-CM induced branching tubular structures with clear lumens, consistent with the existence of other soluble factors crucial for the formation and/or maintenance of branching tubular structures with lumens in vitro .
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 1997
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  • 9
    Online Resource
    Online Resource
    Proceedings of the National Academy of Sciences ; 2009
    In:  Proceedings of the National Academy of Sciences Vol. 106, No. 43 ( 2009-10-27), p. 18333-18338
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 106, No. 43 ( 2009-10-27), p. 18333-18338
    Abstract: With age, T-cell generation from the thymus is much reduced, yet a substantial naïve T-cell pool is maintained even in aged animals, suggesting that naïve T cells either persist longer or turn over faster to maintain T-cell homeostasis. We found that with age, naïve CD4 T cells became progressively longer-lived. Their longer lifespan did not depend on recognition of self-peptide/class II. Newly generated naïve T cells derived from aged stem cells had a shorter lifespan, like that of young naïve T cells. Conversely, naïve CD4 T cells derived from middle-aged thymectomized mice were longer-lived in vivo, and their development of functional defects was accelerated. These observations suggest that naïve T cells develop their longer lifespan during their sojourn in the periphery. Increased longevity of naïve CD4 T cells correlated well with reduced expression of proapoptotic molecule Bim. We suggest that the intrinsic increase in longevity helps maintain naïve T-cell homeostasis but facilitates the development of functional defects in mice.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2009
    detail.hit.zdb_id: 209104-5
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  • 10
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 113, No. 36 ( 2016-09-06)
    Abstract: Targeting glutamine metabolism via pharmacological inhibition of glutaminase has been translated into clinical trials as a novel cancer therapy, but available drugs lack optimal safety and efficacy. In this study, we used a proprietary emulsification process to encapsulate bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide (BPTES), a selective but relatively insoluble glutaminase inhibitor, in nanoparticles. BPTES nanoparticles demonstrated improved pharmacokinetics and efficacy compared with unencapsulated BPTES. In addition, BPTES nanoparticles had no effect on the plasma levels of liver enzymes in contrast to CB-839, a glutaminase inhibitor that is currently in clinical trials. In a mouse model using orthotopic transplantation of patient-derived pancreatic tumor tissue, BPTES nanoparticle monotherapy led to modest antitumor effects. Using the HypoxCR reporter in vivo, we found that glutaminase inhibition reduced tumor growth by specifically targeting proliferating cancer cells but did not affect hypoxic, noncycling cells. Metabolomics analyses revealed that surviving tumor cells following glutaminase inhibition were reliant on glycolysis and glycogen synthesis. Based on these findings, metformin was selected for combination therapy with BPTES nanoparticles, which resulted in significantly greater pancreatic tumor reduction than either treatment alone. Thus, targeting of multiple metabolic pathways, including effective inhibition of glutaminase by nanoparticle drug delivery, holds promise as a novel therapy for pancreatic cancer.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2016
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    detail.hit.zdb_id: 1461794-8
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