In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 116, No. 51 ( 2019-12-17), p. 26020-26028
Abstract:
The voltage-sensing phosphatase (VSP) is a unique protein that shows voltage-dependent phosphoinositide phosphatase activity. Here we report that VSP is activated in mice sperm flagellum and generates a unique subcellular distribution pattern of PtdIns(4,5)P 2 . Sperm from VSP −/− mice show more Ca 2+ influx upon capacitation than VSP +/− mice and abnormal circular motion. VSP-deficient sperm showed enhanced activity of Slo3, a PtdIns(4,5)P 2 -sensitive K + channel, which selectively localizes to the principal piece of the flagellum and indirectly enhances Ca 2+ influx. Most interestingly, freeze-fracture electron microscopy analysis indicates that normal sperm have much less PtdIns(4,5)P 2 in the principal piece than in the midpiece of the flagellum, and this polarized PtdIns(4,5)P 2 distribution disappeared in VSP-deficient sperm. Thus, VSP appears to optimize PtdIns(4,5)P 2 distribution of the principal piece. These results imply that flagellar PtdIns(4,5)P 2 distribution plays important roles in ion channel regulation as well as sperm motility.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.1916867116
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2019
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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