In:
The Journal of Neuroscience, Society for Neuroscience, Vol. 18, No. 8 ( 1998-04-15), p. 2914-2922
Abstract:
α-Latrotoxin (α-Ltx), a component of black widow spider venom, stimulates secretion from nerve terminals and from PC12 cells. In this study we examine the effects of expression of a newly cloned Ca 2+ -independent receptor for α-Ltx (CIRL) on secretion from bovine chromaffin cells. We first characterized the effect of α-Ltx on secretion from untransfected cells. α-Ltx, by binding in a Ca 2+ - independent manner to an endogenous receptor, causes subsequent Ca 2+ -dependent secretion from intact cells. The stimulation of secretion is correlated with Ca 2+ influx caused by the toxin. In permeabilized cells in which the Ca 2+ concentration is regulated by buffer, α-Ltx also enhances Ca 2+ -dependent secretion, indicating a direct role of the endogenous receptor in the secretory pathway. Expression of CIRL increased the sensitivity of intact and permeabilized cells to the effects of α-Ltx, demonstrating that this protein is functional in coupling to secretion. Importantly, in the absence of α-Ltx, the expression of CIRL specifically inhibited the ATP-dependent component of secretion in permeabilized cells without affecting the ATP-independent secretion. This suggests that this receptor modulates the normal function of the regulated secretory pathway and that α-Ltx may act by reversing the inhibitory effects of the receptor.
Type of Medium:
Online Resource
ISSN:
0270-6474
,
1529-2401
DOI:
10.1523/JNEUROSCI.18-08-02914.1998
Language:
English
Publisher:
Society for Neuroscience
Publication Date:
1998
detail.hit.zdb_id:
1475274-8
SSG:
12
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