In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 98, No. 6 ( 2001-03-13), p. 3282-3287
Abstract:
Serological expression cloning of antigens eliciting a humoral
immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a
new member of the cancer/testis family. To identify the human homologue of pem , mouse pem sequences and pem -related expressed sequence tags from human testis
were used as PCR primers for amplification using human testis cDNA. However, rather than pem , another gene, designated OY-TES-1 , was isolated and found to be the human
homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps
to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1- related genes in
the human genome. In normal tissues, OY-TES-1 mRNA was
expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung,
liver, and colon cancers, expressed OY-TES-1 .
Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera
reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of
antigens and that OY-TES-1 is immunogenic in humans.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.041625098
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2001
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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