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  • 1
    Online Resource
    Online Resource
    Border Control—A Membrane-Linked Interactome of Arabidopsis
    American Association for the Advancement of Science (AAAS) ; 2014
    In:  Science Vol. 344, No. 6185 ( 2014-05-16), p. 711-716
    Details
    In: Science, American Association for the Advancement of Science (AAAS), Vol. 344, No. 6185 ( 2014-05-16), p. 711-716
    Abstract: Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10 6 pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis . Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, 〉 99% of the interactions were previously unknown. Interactions were confirmed at a rate of 32% in orthogonal in planta split–green fluorescent protein interaction assays, which was statistically indistinguishable from the confirmation rate for known interactions collected from literature (38%). Regulatory associations in membrane protein trafficking, turnover, and phosphorylation include regulation of potassium channel activity through abscisic acid signaling, transporter activity by a WNK kinase, and a brassinolide receptor kinase by trafficking-related proteins. These examples underscore the utility of the membrane/signaling protein interaction network for gene discovery and hypothesis generation in plants and other organisms.
    Type of Medium: Online Resource
    ISSN: 0036-8075 , 1095-9203
    URL: Article
    DOI: 10.1126/science.1251358
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2014
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  • 2
    Online Resource
    Online Resource
    Boosting Chinese Question Answering with Two Lightweight Methods : ABSPs and SCO-QAT
    Association for Computing Machinery (ACM) ; 2008
    In:  ACM Transactions on Asian Language Information Processing Vol. 7, No. 4 ( 2008-11), p. 1-29
    Details
    In: ACM Transactions on Asian Language Information Processing, Association for Computing Machinery (ACM), Vol. 7, No. 4 ( 2008-11), p. 1-29
    Abstract: Question Answering (QA) research has been conducted in many languages. Nearly all the top performing systems use heavy methods that require sophisticated techniques, such as parsers or logic provers. However, such techniques are usually unavailable or unaffordable for under-resourced languages or in resource-limited situations. In this article, we describe how a top-performing Chinese QA system can be designed by using lightweight methods effectively. We propose two lightweight methods, namely the Sum of Co-occurrences of Question and Answer Terms (SCO-QAT) and Alignment-based Surface Patterns (ABSPs). SCO-QAT is a co-occurrence-based answer-ranking method that does not need extra knowledge, word-ignoring heuristic rules, or tools. It calculates co-occurrence scores based on the passage retrieval results. ABSPs are syntactic patterns trained from question-answer pairs with a multiple alignment algorithm. They are used to capture the relations between terms and then use the relations to filter answers. We attribute the success of the ABSPs and SCO-QAT methods to the effective use of local syntactic information and global co-occurrence information. By using SCO-QAT and ABSPs, we improved the RU-Accuracy of our testbed QA system, ASQA, from 0.445 to 0.535 on the NTCIR-5 dataset. It also achieved the top 0.5 RU-Accuracy on the NTCIR-6 dataset. The result shows that lightweight methods are not only cheaper to implement, but also have the potential to achieve state-of-the-art performances.
    Type of Medium: Online Resource
    ISSN: 1530-0226 , 1558-3430
    URL: Article
    DOI: 10.1145/1450295.1450297
    Language: English
    Publisher: Association for Computing Machinery (ACM)
    Publication Date: 2008
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  • 3
    Online Resource
    Online Resource
    Loss of corepressor PER2 under hypoxia up-regulates OCT1-mediated EMT gene expression and enhances tumor malignancy
    Proceedings of the National Academy of Sciences ; 2013
    In:  Proceedings of the National Academy of Sciences Vol. 110, No. 30 ( 2013-07-23), p. 12331-12336
    Details
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 110, No. 30 ( 2013-07-23), p. 12331-12336
    Abstract: The circadian clock gene Period2 ( PER2 ) has been suggested to be a tumor suppressor. However, detailed mechanistic evidence has not been provided to support this hypothesis. We found that loss of PER2 enhanced invasion and activated expression of epithelial-mesenchymal transition (EMT) genes including TWIST1 , SLUG, and SNAIL . This finding was corroborated by clinical observation that PER2 down-regulation was associated with poor prognosis in breast cancer patients. We further demonstrated that PER2 served as a transcriptional corepressor, which recruited polycomb proteins EZH2 and SUZ12 as well as HDAC2 to octamer transcription factor 1 (OCT1) (POU2F1) binding sites of the TWIST1 and SLUG promoters to repress expression of these EMT genes. Hypoxia, a condition commonly observed in tumors, caused PER2 degradation and disrupted the PER2 repressor complex, leading to activation of EMT gene expression. This result was further supported by clinical data showing a significant negative correlation between hypoxia and PER2. Thus, our findings clearly demonstrate the tumor suppression function of PER2 and elucidate a pathway by which hypoxia promotes EMT via degradation of PER2.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
    URL: Article
    DOI: 10.1073/pnas.1222684110
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2013
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  • 4
    Online Resource
    Online Resource
    Multidecadally resolved polarity oscillations during a geomagnetic excursion
    Proceedings of the National Academy of Sciences ; 2018
    In:  Proceedings of the National Academy of Sciences Vol. 115, No. 36 ( 2018-09-04), p. 8913-8918
    Details
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 115, No. 36 ( 2018-09-04), p. 8913-8918
    Abstract: Polarity reversals of the geomagnetic field have occurred through billions of years of Earth history and were first revealed in the early 20th century. Almost a century later, details of transitional field behavior during geomagnetic reversals and excursions remain poorly known. Here, we present a multidecadally resolved geomagnetic excursion record from a radioisotopically dated Chinese stalagmite at 107–91 thousand years before present with age precision of several decades. The duration of geomagnetic directional oscillations ranged from several centuries at 106–103 thousand years before present to millennia at 98–92 thousand years before present, with one abrupt reversal transition occurring in one to two centuries when the field was weakest. These features indicate prolonged geodynamo instability. Repeated asymmetrical interhemispheric polarity drifts associated with weak dipole fields likely originated in Earth’s deep interior. If such rapid polarity changes occurred in future, they could severely affect satellites and human society.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
    URL: Article
    DOI: 10.1073/pnas.1720404115
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2018
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    detail.hit.zdb_id: 1461794-8
    SSG: 11
    SSG: 12
    Location Call Number Limitation Availability
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