In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 93, No. 25 ( 1996-12-10), p. 14468-14473
Abstract:
The products of the recF, recO, and recR genes are thought to interact and assist RecA in
the utilization of single-stranded DNA precomplexed with single-stranded DNA binding protein (Ssb) during synapsis. Using
immunoprecipitation, size-exclusion chromatography, and Ssb protein affinity chromatography in the absence of any nucleotide cofactors, we
have obtained the following results: ( i ) RecF interacts
with RecO, ( ii ) RecF interacts with RecR in the presence
of RecO to form a complex consisting of RecF, RecO, and RecR (RecF–RecO–RecR); ( iii ) RecF interacts with Ssb
protein in the presence of RecO. These data suggested that RecO mediates the interactions of RecF protein with RecR and with Ssb
proteins. Incubation of RecF, RecO, RecR, and Ssb proteins resulted in the formation of RecF–RecO–Ssb complexes; i.e., RecR was excluded.
Preincubation of RecF, RecO, and RecR proteins prior to addition of Ssb protein resulted in the formation of complexes consisting of RecF,
RecO, RecR, and Ssb proteins. These data suggest that one role of RecF is to stabilize the interaction of RecR with RecO in the presence of
Ssb protein. Finally, we found that interactions of RecF with RecO are lost in the presence of ATP. We discuss these results to explain how
the RecF–RecO–RecR complex functions as an anti-Ssb factor.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.93.25.14468
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1996
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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