In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 76, No. 8 ( 1979-08), p. 3880-3884
Abstract:
The ability of N,N ′-diphenylurea (Ph 2 urea) to substitute for cytokinin-active adenine derivatives in promoting callus growth of Phaseolus lunatus has been examined. In general, Ph 2 urea stimulated callus growth at high concentrations, although the growth of most callus tissues was irregular. Variability in the sensitivity and uniformity of the growth response to Ph 2 urea was found among different genotypes of P. lunatus . Most importantly, tissues cultured on Ph 2 urea-containing medium for one passage had acquired the ability to proliferate in subsequent passages in the absence of either Ph 2 urea or cytokinin-active adenine derivatives. Corresponding tissues maintained on kinetin-containing medium remained cytokinin-dependent. It appears that the effect of Ph 2 urea in promoting the growth of P. lunatus callus tissue resides in its ability to induce cytokinin autonomy. This result suggests that the cytokinin activity of Ph 2 urea may be due to promotion of endogenous cytokinin biosynthesis in the bioassay systems in which it is active.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.76.8.3880
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1979
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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