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  • Criminology  (2)
  • Medicine  (2)
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  • Medicine  (2)
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  • 1
    Online Resource
    Online Resource
    Wiley ; 2022
    In:  Journal of Forensic Sciences Vol. 67, No. 1 ( 2022-01), p. 321-327
    In: Journal of Forensic Sciences, Wiley, Vol. 67, No. 1 ( 2022-01), p. 321-327
    Abstract: In this study, we aimed to explore the possibility of DNA analysis of areca nut as material evidence and the value of short tandem repeat (STR) typing of areca nut as material evidence under the condition of simulating external environment. In this study, water soaking, soil burial, sun exposure, and wet environment were used to treat areca nut residues. Chelex 100 was used to extract DNA, the PowerPlex21 kit to amplify, and the ABI PRISM ® 310 Genetic Analyzer to analyze the DNA of areca nut residues. DNA and STR typing were performed to analyze the residue after chewing. The results showed that the number of residual sites decreased with time under the conditions of water soaking, soil burial, sun exposure, and wet environment. Thus, areca nut can be used as forensic material evidence for DNA analysis and individual identification.
    Type of Medium: Online Resource
    ISSN: 0022-1198 , 1556-4029
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2026357-0
    SSG: 2,1
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Wiley ; 2014
    In:  Journal of Forensic Sciences Vol. 59, No. 3 ( 2014-05), p. 723-728
    In: Journal of Forensic Sciences, Wiley, Vol. 59, No. 3 ( 2014-05), p. 723-728
    Abstract: Shahtoosh, the down hair of the T ibetan antelope ( P antholops hodgsonii ), is the noblest and most expensive wool in the world. The population of the animal has declined dramatically due to commercial poaching for the fiber. Traditional inspection for detection of shahtoosh has been performed by microscopic analysis. We developed a T aq M an real‐time PCR ‐based DNA analysis method for identifying shahtoosh fibers. A set of probe and primers for the mitochondrial 12S ribosomal RNA gene that binds specifically to T ibetan antelope DNA was designed. A signal was detected with sensitivity to the 1:10,000 dilution of shahtoosh DNA . A fiber mixture of 1% of shahtoosh mixed with cashmere and even a single fiber can be detected with this method. The method is faster, more cost‐effective and more sensitive than other traditional sequencing methods and can be directly applied to identify shahtoosh and its processed products, which will be of value in illegal trade investigations.
    Type of Medium: Online Resource
    ISSN: 0022-1198 , 1556-4029
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2026357-0
    SSG: 2,1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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