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  • Jewish studies  (34)
  • 1
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    Online Resource
    Characterization and expression analysis of heat shock protein 90β from golden pompano ( Trachinotus ovatus , Linnaeus 1758) in responses to stock density stress and Cryptocaryon irritans infection
    SAABRON PRESS ; 2021
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 73 ( 2021-10-15)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-10-15)
    Abstract: Heat shock protein 90 family, a class highly conserved protein and important molecular chaperone and its plays a role in enhancing animal adaptability to environmental stress and improving immunity. In this study, a full-length HSP90β cDNA (ToHSP90β) sequence was identified from Trachinotus ovatus. The cDNA of ToHSP90β was 2648 base-pairs (bp) long and contained a 5′-terminal untranslated region of 130 bp, a 3′-terminal untranslated region of 334 bp, and the open reading frame length 2184 bp, which can encode 728 amino acids. The ToHSP90β has a heat shock protein 90 family signature. The ToHSP90β amino acid sequence has high homology with other fish HSP90β amino acids (92.5-99.17%) and contains five conserved signal regions and a structural feature of the cytoplasmic HSP90 family. Phylogenetic tree analysis indicated that ToHSP90β was most closely related to Seriola dumerili HSP90β. Tissues expression analysis showed that the ToHSP90β was expressed in all nine tissues of T. ovatus, and its expression was the highest in the intestine. The stocking density significantly affected the expression of ToHSP90β in the kidney and brain tissues, and its expression levels increased significantly with increasing stocking densities, indicating that it plays an important regulatory role in the process of T. ovatus’ chronic stress response. After Cryptocaryon irritans infection, the mRNA expression of ToHSP90β was significantly increased in liver and kidney tissues, indicating that ToHSP90β would be involved in the immune regulatory response. This study was the first to analyze the expression pattern of HSP90β mRNA in T. ovatus tissues and their expression responses to stocking density stress and C. irritans infection. To provide a theoretical basis for further study of the function of fish HSP90 and its physiological mechanism in environmental stress and parasite infection.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.29485
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
    detail.hit.zdb_id: 291787-7
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  • 2
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    Evaluation of Berberine’s Algicidal Effects on Toxic Microcystis aeruginosa Growth using a Double Fluorescein Staining Method
    SAABRON PRESS ; 2014
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 66 ( 2014-1-1)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 66 ( 2014-1-1)
    Abstract: An accurate and convenient method for determining Microcystis cell viability is necessary to control blooms in small aquaculture water bodies. The fluorescein diacetate-propidium iodide (FDA-PI) staining method was developed to determine the viability of toxic Microcystis aeruginosa (FACHB905). Live M. aeruginosa 905 cells, stained with FDA, emitted bright green fluorescence after excitation at 470 nm. Dead cells, stained with PI, produced bright red fluorescence after excitation at 540 nm. Berberine inhibited M. aeruginosas 905 growth and the inhibition rate determined by FDA-PI fluorescein staining and counting on a dark field was much higher than when determined by counting on a bright field because some berberine-killed cells were misidentified as live cells. Thus, less berberine is needed to control Microcystis bloom when the accurate and reliable FDA-PI fluorescein staining method is used to judge the viability of the algae cells.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.20758
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2014
    detail.hit.zdb_id: 291787-7
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  • 3
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    Tracking the Transformation Process of a Pair of Zn(II) Coordination Clusters: Crystallography and Mass Spectrometry
    Wiley ; 2019
    In:  Israel Journal of Chemistry Vol. 59, No. 3-4 ( 2019-03), p. 286-291
    Details
    In: Israel Journal of Chemistry, Wiley, Vol. 59, No. 3-4 ( 2019-03), p. 286-291
    Abstract: Two zinc clusters Zn 4 (H 3 L) 4 (NO 3 ) 4 ⋅5H 2 O ( Zn 4 , H 4 L=(1,2‐bis(1H‐benzo[d]imidazol‐2‐yl)ethane‐1,2‐diol) and [Zn 5 (H 2 L′) 6 ](NO 3 ) 4 ]⋅8H 2 O⋅2CH 3 OH ( Zn 5 , H 3 L′=(1,2‐bis(benzo[d] imidazol‐2‐yl)‐ethenol) have been obtained by the reaction of Zn(NO 3 ) 2 ⋅6H 2 O with H 4 L at 80 °C or 140 °C under solvothermal condition. Powder X‐ray Diffraction (PXRD) of precipitate and Electrospray Ionization Mass Spectrometry (ESI‐MS) of reaction solution revealed the existence of transformation behavior from Zn 4 to Zn 5 by increasing the temperature from 80 °C to 140 °C, or directly heating Zn 4 at 140 °C via solvothermal reaction. Here we proposed a possible mechanism involves split process of Zn 4 and reassembly to form Zn 5 . ESI‐MS for single crystals revealed [Zn 4 (H 3 L) 4 −3H] + splits to [Zn(H 3 L)] + via [Zn 2 (H 3 L) 2 −H] + . Time dependent ESI‐MS of reaction solution revealed the [Zn(H 2 L′)] + →[Zn 2 (H 2 L′) 2 −H] + →[Zn 5 (H 2 L′) 6 −H] 3+ stepwise assembly. It also has been captured the in situ reaction mainly occurs in the step of [Zn(H 3 L)] + to [Zn(H 2 L′)] + .
    Type of Medium: Online Resource
    ISSN: 0021-2148 , 1869-5868
    URL: Issue
    URL: Article
    DOI: 10.1002/ijch.v59.3-4
    DOI: 10.1002/ijch.201800156
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2066481-3
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  • 4
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    Characterization, functional analysis and antibacterial activities of theromacin from the Akoya pearl oyster Pinctada fucata
    SAABRON PRESS ; 2021
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 73 ( 2021-12-26)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-12-26)
    Abstract: The Akoya Pearl oyster Pinctada fucata is distributed over the coastal area of South China and is the most popular farming shellfish for seawater pearl production. Antimicrobial peptides (AMPs) could inhibit or kill pathogenic microorganisms. Theromacin has been proven to be an antimicrobial peptide, which plays an essential role in the body’s immune system. The AMP gene to be identified was encoding theromacin in the pearl oyster Pinctada fucata (PoAP). The full-length PoAP cDNA contains 522 bp and consists of a 5’-UTR of 6 bp, an unusually long 3’-UTR of 749 bp, and an open reading frame (ORF) of 375 bp that encodes a 124 amino acid peptide with a molecular weight of 13.67 kDa and the theoretical isoelectric point of 9.25. Homology analysis of the deduced amino acid sequence of the PoAP with other known theromacin sequences by MatGAT software revealed that the PoAP shared 29.0%-46.8% similarity to the other known theromacin sequences. Signal P-N program showed that PoAP contains 33 amino acid signal peptides and a mature peptide located at amino acids 34-124; the mature peptide contains 12 cysteine residues and 13 alkaline amino acid residues with a molecular weight of 13.67 kDa and the theoretical isoelectric point of 9.25. The tempered program suggests that PoAP is a membrane protein with one transmembrane helix between amino acids 13 and 34. The PoAP gene was linked into prokaryotic vector pET-32α, and the PoAP fusion protein with 31 kDa molecular mass was successfully expressed in Escherichia coli BL21. Using His-Bind Purification Kit Protocol purifies the antimicrobial peptides recombinant protein and compares the bacteriolysis effect on Gram-positive and Gram-negative bacteria using the purified protein. The results show that PoAP proteins had a bacteriolysis effect on the Gram-negative bacteria but on Gram-positive organisms. The antibacterial peptides theromacin seem to play a crucial antibacterial function in the immune responses of pearl oysters.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.31132
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
    detail.hit.zdb_id: 291787-7
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  • 5
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    Variation of bacterial communities in black tiger shrimp (Penaeus monodon) with different growth performances
    SAABRON PRESS ; 2021
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 73 ( 2021-12-13)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-12-13)
    Abstract: The intestinal bacteria of aquatic animals are closely related to their health and growth status. Penaeus monodon has become an important aquaculture species in the world because of its high nutritional value and market to demand. However, knowledge of bacterial communities on the growth phenotype of P. monodon is still limited. In this research, the bacterial community were comprehensively analyzed by using Miseq sequencing technology and bioinformatics. The results indicated that 29 phyla and 468 genera were identified by the taxonomic sequence. The data showed that the composition of microbial community was statistically different to fast-growing shrimps and slow-growing shrimps. There were significant differences in intestinal bacteria at the genus level. Lactococcus, Limnothrix, and Arthrospira had more abundance in the gut of fast-growing shrimps, which may promote the growth of P. monodon by affecting enzyme activities and improving immune response. Nautella, Shimia, Pseudoalteromonas, Aliiroseovarius, Albimonas, Tessaracoccus had more abundance in the gut of slow-growing shrimps. Nautella had the ability to express virulence by stimulated by environmental factors, thereby increasing the susceptibility to infection, which may have a negative impact on healthy growth of shrimps. Pseudoalteromonas, Aliiroseovarius and Shimia were related to the pathogenicity of shrimp, which may be an important factor affecting the growth of shrimp. Overall, this study demonstrates that the bacterial community associated with P. monodon was variation on different growth performance. These results can provide information on microbiome characteristics involved in the asynchronous growth of shrimp.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.30754
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
    detail.hit.zdb_id: 291787-7
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  • 6
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    Difference analysis of gut microbiota of golden pompano Trachinotus ovatus (Linnaeus, 1758) with the unsynchronized growth by feeding fermented soybean meal
    SAABRON PRESS ; 2021
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 73 ( 2021-4-16)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-4-16)
    Abstract: Abstract Golden pompano (Trachinotus ovatus) is a commercially important marine fish and is widely cultured in the coastal area of South China. We explored the distribution difference of intestinal microflora with the same growth condition and the difference of gut microflora with different growth rates fed with fermented soybean meal (FSM) and fish meal (FM) primary protein source. After 8 weeks of the breeding experiment, select significant growth fish (Zcd, 156.04 ± 1.04 g) average growth fish (Zc, 120.01 ± 1.05 g) stagnant growth fish (Zcx, 98.03 ± 1.08 g) in FSM and select average growth fish (Dc, 158.03 ± 1.02 g) in FM. Sequencing results show that a total of 86303 sequences had been obtained in the intestine of Trachinotus ovatus. The number of OTUs that can be annotated to the database was 2315 (98.97%). At the phylum level, Proteobacteria, Tenericutes, Spirochaetes and Firmicutes were the predominant phyla. Alpha diversity and beta diversity analyses show that gut microbes’ richness and diversity in the Dc group were the highest. The diversity of gut microbiota in the FSM: Zc 〉 Zcd 〉 Zcx. At the genus classification level, the differences between FM and FSM were mainly in Gueglia, Grimontia, Klebsiella, Alistipes, Staphylococcus, and Parasutterella (p 〈 0.05). Alistipes and Staphylococcus were the different genera between Dc and Zcd (p 〈 0.05). We found significant differences in the relative abundance of Ruegeria between the Zc and Zcd groups. Also, significant differences were found in the distribution of Brevinema, Ruegeria, Unidentified-Lachnospiraceae, Blautia, and Bacteroides between Zc and Zcx groups. The results showed that fermented soybean meal had an essential effect on gut microflora of T. ovatus with the unsynchronized growth.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.22213
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
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  • 7
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    Analysis of morphological differences among different populations of golden pompano ( Trachinotus ovatus )
    SAABRON PRESS ; 2023
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 75, No. 1 ( 2023-5-17)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 75, No. 1 ( 2023-5-17)
    Abstract: To explore the external morphological differences of golden pompano in different geographical populations, eight quantitative traits of 210 samples from seven golden pompano populations were measured. Multivariate statistical methods, such as principal component analysis, discriminant analysis, cluster analysis, and One-way ANOVA, were used to compare morphological differences among the populations. Principal component analysis extracted the top five principal components with a cumulative contribution rate of 85.79%, of which the first three principal components could explain seven morphological features. The principal component scatter plot showed that the NH, CH, and LL populations had similar morphology. Using the stepwise discriminant method to establish the classification and discrimination functions of the seven populations, the discrimination accuracy of the DL population was 93.3% for P1 and 87.5% for P2, which was the highest, and the comprehensive discrimination rate was 71.4%. The clustering relationship diagram showed that the populations were divided into three branches, and the CH and NH populations were closest. In contrast, the DL and HF populations were farthest from the other populations. One-way ANOVA showed significant differences ( P 〈 0.05) among all traits of the populations, and the morphological differences between the HX and DL populations were the largest. The results of this study showed specific differences in the external morphology of golden pompano among different populations.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.75267
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2023
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  • 8
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    Histological study on development of the digestive system of longfin batfish (Platax teira)
    SAABRON PRESS ; 2020
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 72 ( 2020-9-28)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 72 ( 2020-9-28)
    Abstract: The ontogeny of the digestive tract in Platax teira was studied by means of optical microscopy from hatching to 30 days post-hatching. Collect fertilized eggs from P. teira broodstock, fertilize the eggs to hatch in hatching net bags, and rear the larvae in higher-place ponds. Observe the morphology and structure of the digestive tract of larvae 1-30 days post-hatching using tissue slicing technique. The results showed that the oral cavity of the larvae was closed and had not yet begun to differentiate at 1day post-hatching. Its digestive tract was close to the abdominal wall, and the yolk sac was completely dyed dark red. At 4 days post-hatching, the oropharyngeal cavity, esophagus, and stomach of the larvae had been communicated, and their oral cavity opened and food intake begins, entering the mixed period of endogenous nutrition and exogenous nutrition. Thereafter, the yolk sac gradually decreased to disappear, the esophagus, stomach, and intestines continued to differentiate, and their functions gradually improved. During 25-30 days post-hatching, the structure of the digestive tract of the larvae didn’t change much. Due to the increase in the size of the larvae, most changes in the digestive system are related to the complexity of the tissues and the size of the organs. Specifically, the number of mucosal folds and cells in the esophagus and intestine increases, and the gastric glands and pits in the stomach increase. The number of mucous membrane folds, mucosal epithelial cells and gastric glands in the digestive tract increase with the growth of the larvae, indicating that the development of the digestive system of P. teira is consistent with the growth of larvae, morphological development, feeding, metabolism and other functions consistently, its developmental stage is also adapted to the stages of the early development stage of P. teira.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.21466
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2020
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  • 9
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    The Effect of Dietary Lipid Levels on Growth Performance, Lipid Deposition, and Antioxidant Status of Juvenile Turbot, Scophthalmus maximus, Fed Isonitrogenous and Isoenergetics Diets
    SAABRON PRESS ; 2015
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 67 ( 2015-1-1)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 67 ( 2015-1-1)
    Abstract: The purpose of this study was to investigate the effects of different lipid (fish oil/soybean oil=1:1, w/w) levels in diets with 45% crude protein on growth performance, body composition, and antioxidant status of turbot (Scophthalmus maximus L.). 2040 turbot (39±0.2g) were randomly divided into 4 groups with 3 replicates and fed isonitrogenous and isoenergetic diets with lipid levels of 6.0%(L6.0), 8.5%(L8.5), 11.0%(L11.0), and 13.5%(L13.5) for 56 days. Hepatic lipase (HL) and superoxide dismutase (SOD) activity were evaluated, as well as lipid peroxidation measured as malondialdehyde (MDA). Results showed that no significant difference was observed in feeding rate (FR), protein efficiency ratio (PER) and protein retention, while dietary lipid levels caused a significant increase (P 〈 0.05) in specific growth rate (SGR) The L13.5 group showed the highest lipid and energy retention and this was significantly higher than in other groups; lipid content in whole body and liver increased significantly with increased dietary lipid (P 〈 0.05); the HL and SOD activity, and MDA in the liver all increased significantly (P 〈 0.05)with dietary lipid supplementation in L13.5. In general, high dietary lipid levels enhanced growth rate of turbot, but the increment of growth was due mainly to excessive lipid deposition. Oxidation stress was observed in fish fed the diet containing 13.5% lipid. The optimal level of dietary lipid for good growth and antioxidant status in turbot was found to be 11%.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.20719
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2015
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  • 10
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    Evaluation of translocation impacts on genetic patterns in farmed and naturalized populations of Mytilus galloprovincialis along the China coast: clues from mitochondrial cytochrome c oxidase I sequences
    SAABRON PRESS ; 2021
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 73 ( 2021-9-17)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-9-17)
    Abstract: As an introduced species, Mytilus galloprovincialis has developed into self-sustaining naturalized populations and has been widely cultivated in northern China. The M. galloprovincialis aquaculture industry wholly depends on the movement of naturalized juveniles onto farms. It is, therefore, necessary to understand the genetic effect of continuous spats’ translocation. This study divided 12 localities of M. galloprovincialis along the China coast into three types of populations—farmed, naturalized adjacent farmed, and isolated—to investigate the genetic variation and differentiation. The genetic variability is reflected by haplotype diversity, nucleotide diversity, and the mean number of pairwise differences expressed as farmed populations 〉 naturalized adjacent farmed populations 〉 isolated populations. The Hierarchical analyses and Mantel-test indicated slight divergence between farmed and naturalized populations, northern and southern populations. The farmed and naturalized populations clustered into two separate categories in the neighbor-joining tree except two anthropogenically intervened localities. The present results suggest that the translocation practice positively affected genetic variability and played a vital role in shaping genetic composition. The information obtained in this study provides new insights into the impacts of the translocation culture model of marine mollusks.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.28258
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
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