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  • 1
    Online Resource
    Online Resource
    Effects of selenium adaptation on intestinal morphology, antioxidant-relate genes expression and intestinal microflora of grass carp (Ctenopharyngodon idella)
    SAABRON PRESS ; 2022
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 74 ( 2022-10-26)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 74 ( 2022-10-26)
    Abstract: In the study, the effects of selenium on intestinal tissue morphology, antioxidant-related genes, and intestinal flora of grass carp (Ctenopharyngodon idella) were studied. For this purpose, 180 healthy grass carps (20.0±2.0 g) were randomly divided into three groups with three replicates each: the corresponding amount of anhydrous sodium selenite was added to make experimental water solutions of different concentrations, including 0 μg/L Se4+ (control group), 200 μg/L Se4+ group and 300 μg/L Se4+ group. The experiment was carried out for 42 days. The obtained results showed that: at the end of the experiment, the 200 μg/L Se4+ adaptation can have beneficial effects on the intestinal villi height and goblet cells. The CuZnSOD and CAT genes mRNA levels of grass carp intestine were strongly upregulated in the 200ug/L Se4+ group. 200ug/L selenium could increase the expression level of the Hsp70 gene in the intestinal tract of grass carp after 42 days of adaptation. At the genus level, the most abundant sequence in the gut of Se-treated grass carp was Pseudomonas, while Aeromon, Flavobacterium, and Defluviimonas were more abundant in the control group. In conclusion, this study demonstrates that 200ug/L Se4+ selenium adaptation can positively affect gut morphology and antioxidant responses and can alter the gut microbiota structure of grass carp. The results will provide a theoretical basis for further research on the effect of selenium on aquatic animals.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.39683
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2022
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
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  • 2
    Online Resource
    Online Resource
    Study a natural co-infection case of Largemouth bass ranavirus, Aeromonas vickert , and Aeromonas hydrophila in Micropterus salmoides
    SAABRON PRESS ; 2023
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 75, No. 2 ( 2023-10-9)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 75, No. 2 ( 2023-10-9)
    Abstract: The largemouth bass (LMB) is a significant aquaculture species widely cultivated in China. High-density culture has led to an increased susceptibility of LMB to infection by the opportunistic bacterium Aeromonas. This study investigates a case of natural co-infection with the Largemouth bass ranavirus (LMBV), Aeromonas veronii , and Aeromonas hydrophila in Sichuan Province, China. To identify the causative agents of the infection, two bacterial strains were isolated from diseased LMB specimens and identified through 16S rRNA sequencing as Aeromonas vickert and Aeromonas hydrophila , both of which are gram-negative bacteria. The infection of LMBV was confirmed through polymerase chain reaction (PCR) analysis of tissue samples from the infected fish. After two blind passes of the virus fluid, the typical cytopathological effect (CPE) can be observed. Phylogenetic analysis of the virus sequences showed the highest similarity with LMBV isolates (ON418985 and MW630113) from Guangdong Province, China. This study offers important insights into diagnosing mixed infections in LMB and provides a reference for future research.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.88364
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2023
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
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  • 3
    Online Resource
    Online Resource
    Effects of Dietary Emodin Supplementation on Growth Performance, Non-Specific Immune Responses, and Disease Resistance to Aeromonas hydrophila in Juvenile Wuchang Bream (Megalobrama amblycephala)
    SAABRON PRESS ; 2014
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 66 ( 2014-1-1)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 66 ( 2014-1-1)
    Abstract: An 8-week feeding trial was conducted to investigate the effects of dietary emodin supplementation on growth, non-specific immunity, and protection against Aeromonas hydrophila infection in juvenile Megalobrama amblycephala. A basal diet was supplemented with 0 (control), 15, 30, 60 and 120 mg emodin/kg to formulate five experimental diets. Each diet was randomly allocated to triplicate tanks of fish in a circulating water system (initial average weight 3.49±0.045g, 25 fish per tank). At the end of the feeding trial, fish fed the diet supplemented with 0 and 120 mg emodin/kg had lower weight gain (WG) and specific growth rate (SGR) than those in the other treatment groups, but no significant differences were observed among diets supplemented with emodin from 15 to 60 mg/kg. A significant increase on feed conversion ratio (FCR) of fish fed diet supplemented with 120 mg emodin/kg was observed. The white blood cell count (WBC), respiratory burst activity, superoxide dismutase (SOD) activity, myeloperoxidase (MPO) activity, malondialdehyde (MDA) content, and tumor necrosis factor-α (TNF-α) activity first increased and then decreased with increase of the dietary emodin levels. Fish fed the 30 mg emodin/kg supplemented diet had higher WBC, respiratory burst activity, SOD and TNF-α activity, and lower MDA content, than fish fed diets supplemented with 0 and 120 mg emodin/kg. In the bacteria challenge experiment with A. hydrophila, fish fed a diet supplemented with 30 and 60 mg/kg had a lower cumulative mortality rate than the control group. These results indicated that appropriate dietary emodin supplementation (especially 30 mg emodin/kg diet) could enhance the growth and immune responses of fish and improve resistance to infection by A. hydrophila.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.20738
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2014
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
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  • 4
    Online Resource
    Online Resource
    Effects of Supplemented Dietary Curcumin on Growth and Non-Specific Immune Responses in Juvenile Wuchang Bream (Megalobrama amblycephala)
    SAABRON PRESS ; 2015
    In:  Israeli Journal of Aquaculture - Bamidgeh Vol. 67 ( 2015-1-1)
    Details
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 67 ( 2015-1-1)
    Abstract: The present study was aimed at evaluating the effects of the feed additive curcumin on the growth and non-specific immune responses in juvenile Wuchang bream (Megalobrama amblycephala). Six experimental diets were formulated to contain graded curcumin levels (0, 15, 30, 60, 120 and 240 mg/kg of diet, respectively). After 60 days feeding trial, fish fed the 60 mg/kg curcumin diet had significantly higher weight gain rate (WGR) and specific growth rate (SGR), and significantly lower feed conversion ratio (FCR) than the control group. The number of leucocytes (WBC), interleukin-1 beta (IL-1β), tumor necrosis factor-α (TNF-α), alternative pathway of complement (ACH50), and respiratory burst activity increased with increasing dietary curcumin levels up to 60 mg/kg, and thereafter declined. Significantly lower alanine transaminase (ALT) and aspartate transaminase (AST) activities were observed in fish fed the 60 mg/kg curcumin diet compared to those in the control group. Fish fed with the diets containing 60 and 120 mg/kg curcumin had significantly higher TNF-α and ACH50 activities than those in the control, and the fish fed diets supplemented with 60 and 240 mg/kg curcumin had significantly higher IL-1β activities than those in the control. The results suggest that appropriate dietary curcumin supplementation (60 mg/kg curcumin of diet) significantly improved growth and non-specific immune responses in juvenile M. amblycephala.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    URL: Article
    DOI: 10.46989/001c.20706
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2015
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
    Location Call Number Limitation Availability
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  • 5
    Online Resource
    Online Resource
    Rapid Identification of Terminal Sialic Acid Linkage Isomers by Pseudo‐MS 3 Mass Spectrometry
    Wiley ; 2015
    In:  Israel Journal of Chemistry Vol. 55, No. 3-4 ( 2015-04), p. 412-422
    Details
    In: Israel Journal of Chemistry, Wiley, Vol. 55, No. 3-4 ( 2015-04), p. 412-422
    Abstract: We used pseudo‐triple‐stage tandem MS to rapidly differentiate terminal α(2,3)‐ and α(2,6)‐linked sialic acids of glycopeptides and native glycans. The precursor ions generate important diagnostic fragments to distinguish different terminal linked isomeric glycans and glycopeptides based on collision‐induced dissociation (CID). The diagnostic ions include hexose linked with N ‐acetylhexosamine residues, sialic acid with the loss of water, and sialic acid residues. Their values of mass‐to‐charge ratio ( m / z ) are at 366.14, 274.09, and 292.10, respectively. The relative intensity ratios (RIR) were calculated by the intensity of signals at m / z 274.09 and 292.10 divided by the intensity of the signal at m / z 366.14. Under optimal conditions of an in‐source fragmentation voltage of 70 V and a normalized collision energy of 30 %, the difference in RIR of diagnostic ions between terminal α(2,3)‐linked sialic acid of glycopeptide (1.3) and terminal α(2,6)‐linked sialic acid of glycopeptide ( 〈 0.18) is the largest. Moreover, quantitative calibration curves with different percentages of α(2,3)‐ and α(2,6)‐linked sialoglycopeptides demonstrated that this method applied not only to biantennary N ‐linked glycan, but also to complex and hybrid N ‐linked glycans and O ‐linked glycans. From measurements of the RIR of diagnostic ions, erythropoietin, fetuin, and herceptin have 98, 70, and 100 %, respectively, α(2,3)‐linked N ‐sialic acid of total terminal sugar moieties. N ‐Glycosylation of the antibody‐binding fragment (Fab) moiety of cetuximab has 100 % α(2,6)‐linked N ‐glycolylneuraminic acid terminal glycan linkages. This method can be applied to identify compounds from chemical synthesis. It can also be used for quality evaluation of bio‐similar protein drugs.
    Type of Medium: Online Resource
    ISSN: 0021-2148 , 1869-5868
    URL: Issue
    URL: Article
    DOI: 10.1002/ijch.v55.3/4
    DOI: 10.1002/ijch.201400141
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2066481-3
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