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  • Jewish studies  (2)
  • 1
    In: Israel Journal of Chemistry, Wiley, Vol. 55, No. 3-4 ( 2015-04), p. 412-422
    Abstract: We used pseudo‐triple‐stage tandem MS to rapidly differentiate terminal α(2,3)‐ and α(2,6)‐linked sialic acids of glycopeptides and native glycans. The precursor ions generate important diagnostic fragments to distinguish different terminal linked isomeric glycans and glycopeptides based on collision‐induced dissociation (CID). The diagnostic ions include hexose linked with N ‐acetylhexosamine residues, sialic acid with the loss of water, and sialic acid residues. Their values of mass‐to‐charge ratio ( m / z ) are at 366.14, 274.09, and 292.10, respectively. The relative intensity ratios (RIR) were calculated by the intensity of signals at m / z 274.09 and 292.10 divided by the intensity of the signal at m / z 366.14. Under optimal conditions of an in‐source fragmentation voltage of 70 V and a normalized collision energy of 30 %, the difference in RIR of diagnostic ions between terminal α(2,3)‐linked sialic acid of glycopeptide (1.3) and terminal α(2,6)‐linked sialic acid of glycopeptide ( 〈 0.18) is the largest. Moreover, quantitative calibration curves with different percentages of α(2,3)‐ and α(2,6)‐linked sialoglycopeptides demonstrated that this method applied not only to biantennary N ‐linked glycan, but also to complex and hybrid N ‐linked glycans and O ‐linked glycans. From measurements of the RIR of diagnostic ions, erythropoietin, fetuin, and herceptin have 98, 70, and 100 %, respectively, α(2,3)‐linked N ‐sialic acid of total terminal sugar moieties. N ‐Glycosylation of the antibody‐binding fragment (Fab) moiety of cetuximab has 100 % α(2,6)‐linked N ‐glycolylneuraminic acid terminal glycan linkages. This method can be applied to identify compounds from chemical synthesis. It can also be used for quality evaluation of bio‐similar protein drugs.
    Type of Medium: Online Resource
    ISSN: 0021-2148 , 1869-5868
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2066481-3
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  • 2
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 74 ( 2022-3-13)
    Abstract: Aeromonas allosaccharophila belongs one of the causative agent of Aeromonas disease in teleosts. Here, we reported on multiple outbreaks of A. allosaccharophila with mortality up to 80% in farmed hybrid sturgeon in Sichuan, China, from 2019 to 2021. This was the first study to A. allosaccharophila from diseased hybrid sturgeons by phenotypic characterization and 16S rRNA analysis. The typical clinical signs of these fish were intestinal segments empty and often with yellow mucus or swelling. Histopathological observation of diseased fish infected with A. allosaccharophila showed the intestinal villus structure disappeared and the intestinal mucosa epithelium was necrotic and shed. Experimental infection indicated that A. allosaccharophila was the pathogen responsible for the mortalities. Antibiotic sensitivity test results showed that A. allosaccharophila was resistant to ampicillin, neomycin, tetracycline, and penicillin, while sensitive to sulfamethoxazole, streptomycin, florfenicol, enrofloxacin, and cefoxitin. Our findings provide references for the disease diagnosis and treatment.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2022
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
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