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  • Chen, Defang  (3)
  • Jewish studies  (3)
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  • Jewish studies  (3)
  • 1
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 75, No. 2 ( 2023-12-15)
    Abstract: Streptococcus agalactiae (GBS) is an important Gram-positive pathogen of fish aquaculture worldwide. In this study, we performed a comparative analysis of GBS S03 (serotype III) and S07 (serotype Ia) isolated from Schizothorax spp.. We explored the association between phenotypic antibiotic resistance, virulence, and genomic characteristics. Antimicrobial sensitivity tests on 12 common antibiotics using the disc diffusion method revealed that the GBS S03 resisted seven antibiotics, while GBS S07 showed sensitivity to eleven antibiotics. Pathogenicity analysis demonstrated greater virulence of GBS S07 than S03. Then, the occurrence of antibiotic resistance and virulence genes were identified using the whole-genome sequence (WGS) of GBS S03 and GBS S07. Besides just GBS S03 had gyrA and parC gene mutations, there were mefE , tetO , lnuB , lsaE , APH3’ , and sat-4 resistance genes present only in GBS S03 genome. In addition to 51 virulence genes in both GBS S03 and S07 genomes, GBS S07 carried virulence genes associated with invasion, such as SAN_1519 , rfbA and cylE genes. There was complete concordance between genotypic evidence and phenotypic characteristics. Virulence factors and phylogenetic analysis showed that GBS S03 and human sources shared an extremely close evolutionary relationship. Our findings also provide important proof for using WGS as an effective tool for phenotypic predictions of GBS.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2023
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
    Location Call Number Limitation Availability
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  • 2
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 68 ( 2016-1-1)
    Abstract: Aeromonas spp. are ubiquitous inhabitants of aquatic ecosystems, and are increasingly being reported as important pathogens for aquatic and terrestrial animals, as well as humans. A common bacterial disease caused by A.veronii has appeared in cultured channel catfish producing symptoms of erythema, hemorrhages, skin ulcers and high mortality (˃30%) in China, causing great economic loss. From 2007 to 2013, diseased channel catfish, Ictalurus punctatus, displaying symptoms of septicemia, ulceration, or abdominal dropsy were collected from Southwestern China. The goals of this study were to identify the bacterial strains isolated from diseased fish and to determine the susceptibility of the pathogenic strains to many currently available antimicrobial agents. 18 bacterial isolates were obtained from diseased fish. Based on phenotypic characteristics and 16S rRNA gene sequence analysis, all isolates were identified as A. veronii. These results indicate that the microbiological risk posed by A. veronii is considerable for channel catfish cultured in Southwestern China. Susceptibility of the isolates to antibiotics was tested using the agar dilution method. All 18 A. veronii isolates were sensitive to florfenicol, norfloxacin and chloramphenicol, and were resistant to cefradine, clindamycin, midecamycin, penbritin, and amoxicillin. There were sensitivity diversities of the 18 A. veronii isolates to other test antibiotics such as sulfamethoxazole, deoxycycline, gentamycin, tobramycin, and more. This in vitro study provides enough data to recommend the use of these antibiotics for treating infectious diseases caused by A. veronii.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2016
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
    Location Call Number Limitation Availability
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  • 3
    In: Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 73 ( 2021-3-2)
    Abstract: Aeromonas salmonicida is a fish pathogen that has resulted in significant losses to the aquaculture industry worldwide. In the winter of 2018/2019, a serious outbreak of A. salmonicida infection of grass carp (Ctenopharyngodon idella) occurred in China. Since C. idella do not feed at low water temperatures, antibiotics cannot be delivered orally, thus the use of disinfectants is the only viable option to control the disease in the winter months. Therefore, the aim of this study was to elucidate the inhibitory effects of different disinfectants against A. salmonicida at various temperatures and pH values. The microdilution method was used to determine the minimum inhibitory concentration (MIC) of five disinfectants against A. salmonicida at different temperatures. The results showed that A. salmonicida was highly adaptable to temperatures ranging from 8 to 35 °C and pH values from 5 to 9. Moreover, based on the results, benzalkonium bromide (BB) had the strongest inhibitory effect at every temperature. Since A. salmonicida has strong environment adaptability, BB should be chosen to control the disease at low temperatures.
    Type of Medium: Online Resource
    ISSN: 0792-156X , 0792-156X
    Language: English
    Publisher: SAABRON PRESS
    Publication Date: 2021
    detail.hit.zdb_id: 291787-7
    detail.hit.zdb_id: 2542938-3
    SSG: 21,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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