In:
Journal of Forensic Sciences, Wiley, Vol. 61, No. 2 ( 2016-03), p. 527-533
Abstract:
The ability to determine vitality and estimate the survival period after a wound is critical in routine forensic practice. The mRNA levels of MMP ‐2 and TIMP ‐2 were examined using quantitative real‐time RT ‐ PCR to determine the age of a wound. Furthermore, the colocalization of them with Macrophage Marker, respectively, was detected by double immunofluorescence, and a standardized rat model of skeletal muscle contusion was established. In the antemortem contused groups, a large number of macrophages showed positive staining for MMP ‐2 and TIMP ‐2, and the expression of MMP ‐2 and TIMP ‐2 mRNA increased sharply at 3 days postinjury, with relative quantities of 5.75 and 2.98. No samples in the other groups showed relative quantities of 〉 5.75 and 2.98; therefore, relative quantities exceeding 5.75 and 2.98 were strongly indicated 3 days after contusion. In addition, there was a significant decrease in the relative quantity in the postmortem contused groups, indicating that they were useful for diagnosing vitality.
Type of Medium:
Online Resource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2016.61.issue-2
DOI:
10.1111/1556-4029.13001
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2026357-0
SSG:
2,1
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