In:
Journal of Forensic Sciences, Wiley, Vol. 62, No. 6 ( 2017-11), p. 1603-1612
Abstract:
Microfluidic chips offer significant speed, cost, and sensitivity advantages, but numerous parameters must be optimized to provide microchip electrophoresis detection. Experiments were conducted to study the factors, including sieving matrices (the concentration and type), surface modification, analysis temperature, and electric field strengths, which all impact the effectiveness of microchip electrophoresis detection of DNA samples. Our results showed that the best resolution for ss DNA was observed using 4.5% w/v (7 M urea) lab‐fabricated LPA gel, dynamic wall coating of the microchannel, electrophoresis temperatures between 55 and 60°C, and electrical fields between 350 and 450 V/cm on the microchip‐based capillary electrophoresis (μ CE ) system. One base‐pair resolution could be achieved in the 19‐cm‐length microchannel. Furthermore, both 9947A standard genomic DNA and DNA extracted from blood spots were demonstrated to be successfully separated with well‐resolved DNA peaks in 8 min. Therefore, the microchip electrophoresis system demonstrated good potential for rapid forensic DNA analysis.
Type of Medium:
Online Resource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2017.62.issue-6
DOI:
10.1111/1556-4029.13408
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2026357-0
SSG:
2,1
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