In:
Journal of Forensic Sciences, Wiley, Vol. 65, No. 1 ( 2020-01), p. 209-213
Abstract:
When using non‐ FTA cards in commercial multiplex STR kits for direct PCR , pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non‐ FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep‐n‐Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex ® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex ® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set‐up to analyze reference samples collected using a non‐ FTA card manufactured in Korea.
Type of Medium:
Online Resource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/1556-4029.14147
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2026357-0
SSG:
2,1
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