In:
Journal of Forensic Sciences, Wiley, Vol. 61, No. 6 ( 2016-11), p. 1531-1537
Kurzfassung:
Pulmonary edema is a common finding in fatal methamphetamine intoxication. However, the underlying mechanism remains poorly understood. This study investigated the molecular pathology of alveolar damage involving pulmonary edema in forensic autopsy cases. Seven candidate reference genes ( RPL 13A, YWHAZ , GUSB , SDHA , GAPDH , B2M, and ACTB ) were evaluated in the lung by the geNorm module in qB ase plus software. RPL 13A, YWHAZ , and GUSB were identified as the most stable reference genes. Using these validated reference genes, intrapulmonary mRNA expressions of matrix metalloproteinases ( MMP s), intercellular adhesion molecule‐1 ( ICAM ‐1), claudin‐5 ( CLDN ‐5), and aquaporins ( AQP s) were examined. Relative mRNA quantification using TaqMan real‐time PCR assay demonstrated higher expressions of all markers except for AQP ‐5 in fatal METH intoxication cases. These findings suggested alveolar damage and compensatory response in fatal METH intoxication cases. Systematic analysis of gene expressions using real‐time qPCR is a useful tool in forensic death investigation.
Materialart:
Online-Ressource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2016.61.issue-6
DOI:
10.1111/1556-4029.13199
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2016
ZDB Id:
2026357-0
SSG:
2,1
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