In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 54, No. 1 ( 2004-01-01), p. 183-189
Abstract:
Three novel halotolerant, hydrogenotrophic methanogens, designated strains K1F9705b T , K1F9705c and O1F9704a, were isolated from an estuary in Eriln Shi, Taiwan, and from a nearby marine water aquaculture fishpond. These isolates were irregular cocci that stained Gram-negative. Strains K1F9705b T and K1F9705c were non-motile, but strain O1F9704a was weakly motile with flagella. They were able to use formate and H 2 /CO 2 to form methane, but they could not catabolize acetate, methanol, trimethylamine or secondary alcohols. Acetate was required for cell growth. Tungsten greatly stimulated the growth of strains K1F9705b T and K1F9705c, but did not affect the growth of strain O1F9704a. Optimal pH and temperature for growth of these three isolates were respectively 7·2 and 37 °C. Optimal NaCl concentration for growth was 0·5 % for strain O1F9704a and 1·0 % for strains K1F9705c and K1F9705b T . Moreover, all strains grew well at up to 8–12 % NaCl. Analysis of the 16S rRNA gene revealed that these isolates are members of the genus Methanocalculus , but are distinct from Methanocalculus taiwanensis , Methanocalculus pumilus and Methanocalculus halotolerans , with sequence similarities of 98·4, 98·3 and 98·2 %, respectively. In addition, strain K1F9705b T possessed 85, 80, 37, 29 and 10 % DNA–DNA relatedness to strain K1F9705c, strain O1F9704a, M. pumilus , M. halotolerans and M. taiwanensis , respectively. Analysis of protein profiles and the M r of surface (S)-layer glycoprotein subunits showed that these three new isolates are closely related to, but distinct from, known Methanocalculus species. A novel species, Methanocalculu s chunghsingensis sp. nov., is proposed for strains K1F9705b T , K1F9705c and O1F9704a. The type strain is K1F9705b T (=OCM 772 T =DSM 14646 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.02761-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2004
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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