In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 73, No. 5 ( 2023-05-01)
Abstract:
A Gram-stain-negative, facultative anaerobic, motile, rod-shaped and orange bacterium, designated A06 T , was obtained off the coast of Weihai, PR China. Cells were 0.4–0.5×0.6–1.0 µm in size. Strain A06 T grew at 20–40 °C (optimum, 33 °C), at pH 6.0–8.0 (optimum, pH 6.5–7.0) and in the presence of 0–8 % NaCl (w/v) (optimum, 2 %). Cells were oxidase and catalase positive. Menaquinone-7 was detected as the major respiratory quinone. The dominant cellular fatty acids were identified as C 15:0 2-OH, iso-C 15:0 , anteiso-C 15:0 and iso-C 15:1 ω 6 c . The DNA G+C content of strain A06 T was 46.1 mol%. The polar lipids were phosphatidylethanolamine, one aminolipid, one glycolipid and three unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A06 T is a member of the family Prolixibacteraceae and exhibited the highest sequence similarity to Mangrovibacterium diazotrophicum DSM 27148 T (94.3 %). Based on its phylogenetic and phenotypic characteristics, strain A06 T is considered to represent a novel genus in the family Prolixibacteraceae , for which the name Gaoshiqia gen. nov. is proposed. The type species is Gaoshiqia sediminis sp. nov., with type strain A06 T (=KCTC 92029 T =MCCC 1H00491 T ). The identification and acquisition of microbial species and genes in sediments will help broaden the understanding of microbial resources and lay a foundation for its application in biotechnology. Strain A06 T uses an enrichment method, so the isolation of strain A06 T is of great significance to the enrichment of marine microbial resources.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005855
Language:
English
Publisher:
Microbiology Society
Publication Date:
2023
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Permalink