In:
Journal of Animal Science, Oxford University Press (OUP), Vol. 97, No. Supplement_3 ( 2019-12-05), p. 202-203
Abstract:
The major concern for the pig industry is disease control and prevention, and porcine reproductive and respiratory syndrome (PRRS) is one of the main threats to it. Tonsil is a part of the immune system and its role is to recognize and reject foreign antigens to prevent them from invading the lungs. Interestingly, porcine tonsil can harbor PRRS viruses over 150 days post infection (dpi) with no clinical signs, causing PRRS re-break. This study was conducted to find out variation in levels of gene expression in tonsils and investigate functional roles of differentially expressed genes in tonsils depending on days post infection. We used porcine tonsils from weaned pigs following experimental infection with PRRSV-2 (JA142 strain) at 3, 10, 21, 28, 35 dpi. Based on RNA-seq analysis pipeline, differentially expressed genes (DEGs) were considered significant at False Discovery Rate (FDR) ≤ 0.05 level and above the two-fold change. Comparative analyses of 3 dpi vs. 10 dpi, 3 dpi vs. 21 dpi, 3 dpi vs. 28 dpi, and 3 dpi vs. 35 dpi produced 368, 315, 754, and 336 DEGs, respectively. Then we annotated the functions of each DEG set using DAVID tool. Significant GO terms of the MF, BP, and CC ontologies and KEGG pathways were selected within the limit of FDR & lt; 0.05. Common overrepresented GO terms of all DEG sets were mainly negative regulation of viral genome replication, defense response to virus, negative regulation of type Ⅰ interferon production, and type Ⅰ interferon signaling pathway. Specific GO terms were found such as cellular response to interleukin-1, interferon-gamma-mediated signaling pathway, growth factor activity, and acute-phase response from each DEG set, respectively. It suggested tonsil responded to protect itself from PRRSV infection but further study is required to understand PRRSV persistence in tonsils.
Type of Medium:
Online Resource
ISSN:
0021-8812
,
1525-3163
DOI:
10.1093/jas/skz258.417
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2019
detail.hit.zdb_id:
390959-1
detail.hit.zdb_id:
1490550-4
SSG:
12
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