In:
Physiological Research, Institute of Physiology of the Czech Academy of Sciences
Kurzfassung:
It is well known that antagonists of N-type voltage-gated calcium channels inhibit the evoked quantal release of acetylcholine in amphibian neuromuscular synapses. This, however, does not exclude the functional expression of other types of voltage-gated calcium channels in these nerve terminals. Using immunocytochemistry, we detected the expression of the 1A subunit of P/Q-type calcium channels (that is otherwise typical of mammalian motor nerve endings) in the frog neuromuscular junction. In addition, we demonstrated that the P/Q-type channel blocker ω-agatoxin IVA (20 nM) reduced the action potential-induced calcium transient and significantly decreased both spontaneous and evoked mediator release. Our data indicates the functional expression of P/Q-type calcium channels in the frog motor nerve ending which participate in acetylcholine release.
Materialart:
Online-Ressource
ISSN:
1802-9973
,
0862-8408
DOI:
10.33549/physiolres.932219
Sprache:
Englisch
Verlag:
Institute of Physiology of the Czech Academy of Sciences
Publikationsdatum:
2011
ZDB Id:
2100162-5
SSG:
12
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