In:
ELECTROPHORESIS, Wiley, Vol. 25, No. 21-22 ( 2004-11), p. 3669-3676
Abstract:
Desorption/ionization on silicon mass spectrometry (DIOS‐MS) is a matrix‐free technique that allows for the direct desorption/ionization of low‐molecular‐weight compounds with little or no fragmentation of analytes. This technique has a relatively high tolerance for contaminants commonly found in biological samples. DIOS‐MS has been applied to determine the activity of immobilized enzymes on the porous silicon surface. Enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. It is demonstrated that this method can be applied to the screening of enzyme inhibitors. Furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix‐assisted laser desorption/ionization‐time of flight‐MS has been developed.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.v25:21/22
DOI:
10.1002/elps.200406063
Language:
English
Publisher:
Wiley
Publication Date:
2004
detail.hit.zdb_id:
1475486-1
SSG:
12
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