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  • 1
    Online Resource
    Online Resource
    Polyphosphate in Trichodesmium from the low‐phosphorus Sargasso Sea
    Wiley ; 2010
    In:  Limnology and Oceanography Vol. 55, No. 5 ( 2010-09), p. 2161-2169
    Details
    In: Limnology and Oceanography, Wiley, Vol. 55, No. 5 ( 2010-09), p. 2161-2169
    Abstract: Polyphosphate (polyP) is often considered to be the product of luxury uptake in areas of excess phosphorus (P), but can also accumulate in P‐depleted cells in response to P resupply. To test the hypothesis that polyP is present in phytoplankton from oligotrophic systems, the marine diazotroph Trichodesmium was collected from the low‐P surface waters of the Sargasso Sea and assayed with solid‐state 31 P nuclear magnetic resonance spectroscopy. Up to 25% of Trichodesmium cellular P was characterized as polyP, despite physiological data that indicated the colonies were P deplete. This was consistent with culture studies where there were high percentages of polyP under P‐deplete conditions. All Trichodesmium species examined had the genetic machinery to produce and degrade polyP. Trends in the amount of Trichodesmium polyP along the cruise transect showed that allocation of P to polyP was consistently high, and that the ratio of polyP : carbon varied with changes in temperature and mixed‐layer depth. It may be that Trichodesmium was taking advantage of pulses in P supply, and that polyP is a physiological fingerprint of this variability. Additionally, if polyP formation is a common trait in phytoplankton, polyP released from cells could be an additional bioavailable component of the dissolved organic P pool. Taken together, this study highlights the importance of polyP to P cycling and cellular P allocation even in oligotrophic regions.
    Type of Medium: Online Resource
    ISSN: 0024-3590 , 1939-5590
    URL: Article
    DOI: 10.4319/lo.2010.55.5.2161
    Language: English
    Publisher: Wiley
    Publication Date: 2010
    detail.hit.zdb_id: 2033191-5
    detail.hit.zdb_id: 412737-7
    SSG: 12
    SSG: 14
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  • 2
    Online Resource
    Online Resource
    Molecular analysis of the phosphorus starvation response in Trichodesmium spp.
    Wiley ; 2009
    In:  Environmental Microbiology Vol. 11, No. 9 ( 2009-09), p. 2400-2411
    Details
    In: Environmental Microbiology, Wiley, Vol. 11, No. 9 ( 2009-09), p. 2400-2411
    Abstract: The marine diazotroph Trichodesmium is a major contributor to primary production and nitrogen fixation in the tropical and subtropical oceans. These regions are often characterized by low phosphorus (P) concentrations, and P starvation of Trichodesmium could limit growth, and potentially constrain nitrogen fixation. To better understand how this genus responds to P starvation we examined four genes involved in P acquisition: two copies of a high‐affinity phosphate binding protein ( pstS and sphX ) and two putative alkaline phosphatases ( phoA and phoX ). Sequence analysis of these genes among cultured species of Trichodesmium ( T. tenue, T. erythraeum, T. thiebautii and T. spiralis ) showed that they all are present and conserved within the genus. In T. erythraeum IMS101, the expression of sphX , phoA and phoX were sensitive to P supply whereas pstS was not. The induction of alkaline phosphatase activity corresponded with phoA and phoX expression, but enzyme activity persisted after the expression of these genes returned to basal levels. Additionally, nifH (nitrogenase reductase; involved in nitrogen fixation) expression was downregulated under P starvation conditions. These data highlight molecular level responses to low P and lay a foundation for better understanding the dynamics of Trichodesmium P physiology in low‐P environments.
    Type of Medium: Online Resource
    ISSN: 1462-2912 , 1462-2920
    URL: Issue
    URL: Article
    DOI: 10.1111/emi.2009.11.issue-9
    DOI: 10.1111/j.1462-2920.2009.01968.x
    Language: English
    Publisher: Wiley
    Publication Date: 2009
    detail.hit.zdb_id: 2020213-1
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Dissolved inorganic and organic phosphorus uptake in Trichodesmium and the microbial community: The importance of phosphorus ester in the Sargasso Sea
    Wiley ; 2010
    In:  Limnology and Oceanography Vol. 55, No. 3 ( 2010-05), p. 1390-1399
    Details
    In: Limnology and Oceanography, Wiley, Vol. 55, No. 3 ( 2010-05), p. 1390-1399
    Abstract: The dynamics and relative importance of inorganic phosphate (P i ) and dissolved organic phosphorus (DOP) uptake were examined in Trichodesmium and the microbial community. Trichodesmium DOP and P i uptake rates were also compared to literature values from several other taxonomically important groups in the Sargasso Sea. Uptake rates and uptake kinetics of 33 P i and DOP (using alpha‐labeled adenosine‐5′‐triphosphate 33 P‐ATP as a model P ester compound) were assayed during two cruises. The in situ uptake rates suggest that the contribution of P ester to total P uptake can be greater than 25% for Trichodesmium , the microbial community, and key phytoplankton groups ( Prochlorococcus , Synechococcus , picoeukaryotes, and nanoeukaryotes), depending on the location. Based on the kinetics of P i uptake and P ester uptake, Trichodesmium is a poor competitor for P i but a much better competitor for P esters as compared to the microbial community. Trichodesmium growth rates calculated from the P i and P ester uptake parameters suggest that only a small fraction of the P ester pool needs to be bioavailable to allow reasonable growth rates. These data underscore the importance of P esters in supporting production by Trichodesmium in the Sargasso Sea, and provide the first in situ measurements of P ester uptake and uptake kinetics in this important N 2 ‐fixing genus.
    Type of Medium: Online Resource
    ISSN: 0024-3590 , 1939-5590
    URL: Article
    DOI: 10.4319/lo.2010.55.3.1390
    Language: English
    Publisher: Wiley
    Publication Date: 2010
    detail.hit.zdb_id: 2033191-5
    detail.hit.zdb_id: 412737-7
    SSG: 12
    SSG: 14
    Location Call Number Limitation Availability
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    Online Resource
  • 4
    Online Resource
    Online Resource
    Nutrient-regulated transcriptional responses in the brown tide-forming alga Aureococcus anophagefferens : Aureococcus transcriptional response to N and P
    Wiley ; 2011
    In:  Environmental Microbiology Vol. 13, No. 2 ( 2011-02), p. 468-481
    Details
    In: Environmental Microbiology, Wiley, Vol. 13, No. 2 ( 2011-02), p. 468-481
    Type of Medium: Online Resource
    ISSN: 1462-2912
    URL: Article
    DOI: 10.1111/j.1462-2920.2010.02351.x
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2020213-1
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    The Gene Ontology resource: enriching a GOld mine
    Oxford University Press (OUP) ; 2021
    In:  Nucleic Acids Research Vol. 49, No. D1 ( 2021-01-08), p. D325-D334
    Details
    In: Nucleic Acids Research, Oxford University Press (OUP), Vol. 49, No. D1 ( 2021-01-08), p. D325-D334
    Abstract: The Gene Ontology Consortium (GOC) provides the most comprehensive resource currently available for computable knowledge regarding the functions of genes and gene products. Here, we report the advances of the consortium over the past two years. The new GO-CAM annotation framework was notably improved, and we formalized the model with a computational schema to check and validate the rapidly increasing repository of 2838 GO-CAMs. In addition, we describe the impacts of several collaborations to refine GO and report a 10% increase in the number of GO annotations, a 25% increase in annotated gene products, and over 9,400 new scientific articles annotated. As the project matures, we continue our efforts to review older annotations in light of newer findings, and, to maintain consistency with other ontologies. As a result, 20 000 annotations derived from experimental data were reviewed, corresponding to 2.5% of experimental GO annotations. The website (http://geneontology.org) was redesigned for quick access to documentation, downloads and tools. To maintain an accurate resource and support traceability and reproducibility, we have made available a historical archive covering the past 15 years of GO data with a consistent format and file structure for both the ontology and annotations.
    Type of Medium: Online Resource
    ISSN: 0305-1048 , 1362-4962
    URL: Article
    DOI: 10.1093/nar/gkaa1113
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1472175-2
    SSG: 12
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