In:
Photochemistry and Photobiology, Wiley, Vol. 27, No. 3 ( 1978-03), p. 335-341
Abstract:
Abstract. Squid rhodopsin extracted with 2% digitonin (pH 10.5 or 7.0) was excited with a 347 nm light pulse from a mode‐locked ruby laser at room temperature. Within 19 ps after the excitation, absorbance at 430 nm due to hypsorhodopsin increased and subsequently decreased with a decay time of 45 ± 10 ps. Absorbance at 550 nm due to bathorhodopsin increased with a rise time of 50 ± 10 ps. These results are the first observations of hypsorhodopsin at room temperature and clearly show that hypsorhodopsin is a precursor of bathorhodopsin which has been considered to be the earliest photoproduct in the photobleaching process of rhodopsin. Hypsorhodopsin appeared with a rise time of 70 ± 10 ps at 421 nm at liquid nitrogen temperature without any bathorhodopsin being observed during the formation of hypsorhodopsin. An experiment using an N 2 laser showed that squid bathorhodopsin converted to lumirhodopsin with a decay time of about 300 ns at room temperature.
Type of Medium:
Online Resource
ISSN:
0031-8655
,
1751-1097
DOI:
10.1111/php.1978.27.issue-3
DOI:
10.1111/j.1751-1097.1978.tb07609.x
Language:
English
Publisher:
Wiley
Publication Date:
1978
detail.hit.zdb_id:
2048860-9
SSG:
12
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