In:
Bioscience, Biotechnology, and Biochemistry, Informa UK Limited, Vol. 83, No. 5 ( 2019-05-04), p. 876-881
Abstract:
A facile and efficient method has been developed for the optical resolution of racemic jasmonic acid (JA) on a relatively large scale and was successfully utilized for the preparation of optically pure (+)-JA and (−)-JA. We indicated that (+)-JA has lower growth inhibitory activity than (−)-JA in the rice seedling growth test and confirmed in line with an earlier observation that their respective biologically-active forms, (+)-JA-Ile and (−)-JA-Ile, show comparable inhibitory activities. We compared the metabolism of (+)-JA and (−)-JA into (+)-JA-Ile and (−)-JA-Ile, respectively, in the JA-deficient rice cpm2, and found that the exogenously applied (+)-JA was metabolized to the corresponding Ile conjugate less efficiently as compared with (−)-JA. Such metabolic rate difference may cause a discrepancy between biological potencies of (+)-JA and (−)-JA in rice. Abbreviations: FW: fresh weight; Ile: isoleucine; JA: jasmonic acid; JA-Ile: jasmonoyl-l-isoleucine; LC-ESI-MS/MS: liquid chromatography and electrospray ionization tandem mass spectrometry; MeJA: methyl jasmonate; OPDA: 12-oxophytodienoic acid
Type of Medium:
Online Resource
ISSN:
0916-8451
,
1347-6947
DOI:
10.1080/09168451.2019.1569500
Language:
English
Publisher:
Informa UK Limited
Publication Date:
2019
detail.hit.zdb_id:
2110940-0
SSG:
12
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