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  • 1
    Online Resource
    Online Resource
    PSXII-4 Estrus Synchronization and Artificial Insemination in Korean Black Goats Using Frozen-Thawed Semen
    Oxford University Press (OUP) ; 2022
    In:  Journal of Animal Science Vol. 100, No. Supplement_3 ( 2022-09-22), p. 208-209
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 100, No. Supplement_3 ( 2022-09-22), p. 208-209
    Abstract: Korean black goat (Capra hircus coreanae) populations are dwindling and facing genetic erosion due to artificial insemination. Here, we introduced an estrus synchronization method for Korean black goats to enable crossbreeding with non-native breeds on small farms and investigated the application of estrus synchronization and artificial insemination in Korean black goats. Semen was obtained using electro-ejaculation and preserved using a conventional and simple freezing method. Pre- and post-freezing semen was analyzed using computer-assisted sperm analysis, as per the Food Standards Agency 2011 guidelines. The percentage of motile sperm in the electro-ejaculated samples declined significantly after freezing and thawing. However, this did not influence the artificial insemination results. In addition, the incubation period was positively correlated with sperm motility decline in the frozen-thawed sperm samples. Blood progesterone (P4) and estrogen (E2) concentrations were also analyzed to determine the optimal artificial insemination time in estrus-synchronized Korean black goats. The blood samples were collected from a jugular vein at approximately 0900 daily for 5 days. Subsequently, the serum was obtained using centrifugation and stored at -70°C until analysis. Although the P4 and E2 concentrations were generally unaffected by the estrous synchronization method, the controlled internal drug release + prostaglandin F2α + pregnant mare serum gonadotropin method yielded a 42.9% parturition rate after artificial insemination. In the present study, an offspring from an estrus-synchronized Korean black goat was successfully produced by artificial insemination using frozen-thawed semen. The successful cryopreservation of semen for artificial insemination was substantiated with sperm motility and pregnancy rates. In the future, additional studies of estrus synchronization for the artificial insemination of Korean black goats are needed. Our results are expected to significantly contribute to the Korean black goat breeding industry.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skac247.379
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 1490550-4
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  • 2
    Online Resource
    Online Resource
    Adhaeribacter swui sp. nov., isolated from wet mud
    Microbiology Society ; 2018
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 68, No. 4 ( 2018-04-01), p. 1096-1100
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 68, No. 4 ( 2018-04-01), p. 1096-1100
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijsem.0.002631
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2018
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    PSV-26 Effect of feeding regime on meat quality of elk deer (venison) loin during aging
    Oxford University Press (OUP) ; 2020
    In:  Journal of Animal Science Vol. 98, No. Supplement_4 ( 2020-11-30), p. 361-362
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 98, No. Supplement_4 ( 2020-11-30), p. 361-362
    Abstract: The objective of this study was to investigate the effects of feeding regimes on chemical composition, meat quality, and fatty acids composition of elk deer loin (EDL) during aging at 4°C. Sixteen 3-year-old elk hinds were randomly assigned to one of two dietary treatments, grazing pasture (GR) and barn feeding (BF). Eight elks were grazed on pasture with supplementary feed of 1.0% of body weight and the other eight elks were fed 1.0% of concentrate with ad libitum hay for 5 months. After slaughtering, EDL was dissected and aged for 56 days at 4°C in vacuum packaging. Proximate composition, physicochemical characteristics, and fatty acid composition of EDL were determined. Proximate composition of EDL showed no significant difference between feeding regimen and aging period. The pH values of EDL increased with increase of storage days (P & lt; 0.05). Drip loss increased by day 14 compared to initial day and shear force was decreased during aging (P & lt; 0.05). Water holding capacity (WHC) and cooking loss were not significantly affected by feeding regimen or aging period. Lightness (L*) and redness (a*) were decreased on day 56 compared to initial day. Yellowness (b*) of EDL decreased with increase of aging time (P & lt; 0.05). The α-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were higher in GR treatment and increased with aging duration (P & lt; 0.05). The feeding regimens and aging affected on meat quality and fatty acids profiles.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skaa278.634
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 1490550-4
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  • 4
    Online Resource
    Online Resource
    PSIII-14 Genetic Diversity and Haplogroup Distribution of Asian Chicken Populations using Mitochondrial DNA sequence
    Oxford University Press (OUP) ; 2020
    In:  Journal of Animal Science Vol. 98, No. Supplement_4 ( 2020-11-30), p. 236-236
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 98, No. Supplement_4 ( 2020-11-30), p. 236-236
    Abstract: Estimating the genetic diversity and origins is critical for the identification and conservation of valuable animal genetic resources. Mitochondrial DNA (mtDNA) polymorphisms have played a significant role in tracing the origin of specific breeds and the genetic diversity of domestic chicken and other livestock species due to their maternal inheritance, greater rate of substitution relative to unclear genes, and much lower rate of recombination. In this study, we analyzed the mtDNA D-loop region of 457 genomic DNA samples from 28 Asian chicken populations (Korea: 6, Laos: 4, Bangladesh: 3, Cambodia: 3, Indonesia: 3, Vietnam: 2, Kyrgyzstan: 1, Mongolia: 1, Nepal: 1, Sri Lanka: 1 and White Leghorn, Cornish, Rhode Island Red) from ten Asian countries to clarify their genetic diversity and phylogenetic relationships. In addition, 49 reference data (43: Oka et al data, 6: red jungle fowl) were used. We found 82 haplotypes defined by 81 variable sites. Overall haplotype and nucleotide diversity were 0.978±0.01 and 0.0018, respectively. Phylogenetic analyses revealed seven haplogroups (Type A-G). The most of Asian chickens were included in haplogroup A(44.9%), B(22.8%), C(19.3%). Haplogroup F did not belong to any Asian chicken populations (include only 2 reference data). Mongolia chickens were only clustered in haplogroup A and Indonesian chickens did only haplogroup C. About half of Kyrgyzstani chicken populations (43.4%) were clustered in haplogroup E. Vietnamese chicken populations belong to all haplogroup except F. In case of red jungle fowl, G.g.gallus and G.g.bankiva were clustered in haplogroup C. G.g.spadiceus was clusterd in haplogroup E or A. As a result, it was possible to identify the mtDNA diversity and origin of Asian chicken populations. In future studies, it is necessary to understand the origin of each haplogroup by adding more reference data and red jungle fowl sequence data.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skaa278.432
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 1490550-4
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  • 5
    Online Resource
    Online Resource
    CCL20 induced by visfatin in macrophages via the NF-κB and MKK3/6-p38 signaling pathways contributes to hepatic stellate cell activation
    Springer Science and Business Media LLC ; 2020
    In:  Molecular Biology Reports Vol. 47, No. 6 ( 2020-06), p. 4285-4293
    Details
    In: Molecular Biology Reports, Springer Science and Business Media LLC, Vol. 47, No. 6 ( 2020-06), p. 4285-4293
    Abstract: Chemokines interact with hepatic resident cells during inflammation and fibrosis. CC chemokine ligand (CCL) 20 has been reported to be important in inflammation and fibrosis in the liver. We hypothesized that visfatin, an adipocytokine, could play a role in hepatic fibrosis via CCL20. We investigated the effect of visfatin on CCL20 in THP-1 human promonocytic cells and examined the molecular mechanisms involved. Following treatment of THP-1 cells with visfatin, CCL20 expression and secretion were assessed. We assessed the intracellular signaling molecules IKK/NF-κB, JAK2/STAT3, MAPKs, and MKK3/6 by western blotting. We treated THP-1 cells with visfatin and signaling inhibitors, and examined CCL20 mRNA and protein levels. To investigate the effect of visfatin-induced CCL20 expression in hepatic stellate cells (HSCs), LX-2 cells were co-cultured with the culture supernatant of THP-1 cells with or without anti-CCL20 neutralizing antibodies, and fibrosis markers were examined by RT-PCR and immunoblotting. In THP-1 cells, visfatin increased the CCL20 mRNA and protein levels. visfatin increased the activities of the NF-κB, p38, and MLK3/6 signaling pathways but not those of the JAK2/STAT3 and ERK pathways. Visfatin treatment together with an NF-κB, p38, or MLK3 inhibitor reduced the mRNA and protein levels of CCL20. The visfatin-induced CCL20 increased the expression of fibrosis markers and CCR6 in HSCs. Following neutralization of CCL20, the levels of fibrosis markers and CCR6 were decreased. Visfatin increases the expression of CCL20 via the NF-κB and MKK3/6-p38 signaling pathways in macrophages, and visfatin-induced CCL20 expression promotes the fibrosis markers in HSCs.
    Type of Medium: Online Resource
    ISSN: 0301-4851 , 1573-4978
    URL: Article
    DOI: 10.1007/s11033-020-05510-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 1478217-0
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  • 6
    Online Resource
    Online Resource
    Hepatoprotective effects of gemigliptin and empagliflozin in a murine model of diet-induced non-alcoholic fatty liver disease
    Elsevier BV ; 2022
    In:  Biochemical and Biophysical Research Communications Vol. 588 ( 2022-01), p. 154-160
    Details
    In: Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 588 ( 2022-01), p. 154-160
    Type of Medium: Online Resource
    ISSN: 0006-291X
    URL: Article
    DOI: 10.1016/j.bbrc.2021.12.065
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 1461396-7
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  • 7
    Online Resource
    Online Resource
    Monthly Variations in the Intracellular Nutrient Pools of Phytoplankton in Jaran Bay, Korea
    Coastal Education and Research Foundation ; 2018
    In:  Journal of Coastal Research Vol. 85 ( 2018-05), p. 331-335
    Details
    In: Journal of Coastal Research, Coastal Education and Research Foundation, Vol. 85 ( 2018-05), p. 331-335
    Type of Medium: Online Resource
    ISSN: 0749-0208 , 1551-5036
    URL: Article
    DOI: 10.2112/SI85-067.1
    Language: English
    Publisher: Coastal Education and Research Foundation
    Publication Date: 2018
    detail.hit.zdb_id: 53639-8
    detail.hit.zdb_id: 2156089-4
    SSG: 14
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  • 8
    Online Resource
    Online Resource
    PSXI-30 Effects of feeding levels on digestibility, rumen fermentation and blood metabolites of fattening Korean native goats (Capra hircus coreanae)
    Oxford University Press (OUP) ; 2021
    In:  Journal of Animal Science Vol. 99, No. Supplement_3 ( 2021-10-08), p. 485-486
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 99, No. Supplement_3 ( 2021-10-08), p. 485-486
    Abstract: The objective of this study was to investigate the effects of feeding levels on nutrient digestibility, rumen fermentation and blood metabolites of fattening Korean native goats. Five Korean native goat (Capra hircus coreanae) bucks (38.0±1.7 kg) with an average age of 2.4-year-old were randomly allocated to one of five feeding levels (maintenance [M], 1.1 x M, 1.2 x M, 1.4 x M, and 1.6 x M). The experimental design was 5 × 5 Latin square design and all animals were housed individual pens (1.2 m × 0.9 m) for an adaption period of 2 weeks and collection period for 1 weeks. The average daily gain (P & lt; 0.05) and nutrient digestibility (P & lt; 0.01) were increased linearly in goat as feeding level increased. Rumen pH showed no significantly differences among feeding levels. Total volatile fatty acid, acetate and propionate concentrations were increased linearly (P & lt; 0.01) and quadratically (P & lt; 0.01) with increased feeding levels. Butyrate and ammonia nitrogen concentrations were linearly increased with increased feeding levels (P & lt; 0.01), but valerate concentration did not differ among feeding levels. Blood metabolites did not differ among feeding levels except for albumin concentration which was linearly increased with increased feeding levels (P & lt; 0.05). The fecal energy loss was not influenced by feeding levels, but digestible energy was increased linearly (P & lt; 0.01) and quadratically (P & lt; 0.05) with increased feeding levels. The metabolizable energy requirement was estimated using quadratic line model and ranged from 153 to 161 kcal per metabolic body weight based on average daily gain and ME intake. These results found that the increased feeding level might be improve an average daily gain and ruminal fermentation of Korean native goats in fattening phase. These results may also serve as a basis for the establishment of feeding standard of goats in Republic of Korea.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skab235.856
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1490550-4
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  • 9
    Online Resource
    Online Resource
    PSXII-24 Effects of feeding levels on digestibility, rumen fermentation and blood metabolites of growing Korean native goats (Capra hircus coreanae)
    Oxford University Press (OUP) ; 2021
    In:  Journal of Animal Science Vol. 99, No. Supplement_3 ( 2021-10-08), p. 491-491
    Details
    In: Journal of Animal Science, Oxford University Press (OUP), Vol. 99, No. Supplement_3 ( 2021-10-08), p. 491-491
    Abstract: The objective of this study was to investigate the effects of feeding levels on nutrient digestibility, rumen fermentation and blood metabolites of growing Korean native goats. Five growing bucks (19.5±1.4 kg) with an average age of 5 months were randomly allocated to one of five feeding levels [maintenance (M), 1.1 x M, 1.2 x M, 1.4 x M, and 1.6 x M]. The experimental design was 5 × 5 Latin square design and all animals were housed individual pens (1.2 m × 0.9 m) for an adaption period of 2 weeks and collection period for 1 weeks. Rumen samples were collected before morning feeding using oral stomach tube. Nutrient digestibility increased linearly in goat as feeding level increased (P & lt; 0.01), but body weight did not differ among feeding levels. Rumen pH and ammonia nitrogen concentration showed no significantly differences among feeding levels. The total and individual volatile fatty acids concentration increased linearly (P & lt; 0.01) and quadratically (P & lt; 0.05) with increased feeding levels. Blood metabolites did not differ among feeding levels. The fecal energy loss was not influenced by feeding levels, but digestible energy was increased linearly (P & lt; 0.01) and quadratically (P & lt; 0.05) with increased feeding levels. The metabolizable energy requirement was estimated using quadratic line model and ranged from 154 to 163 kcal per metabolic body weight based on average daily gain and ME intake. These results suggest that higher feeding levels of growing goat increases digestibility and nutrient utilization efficiency in the rumen. These results serve as a basis for the establishment of goat feeding programs at the farm scale in the Republic of Korea.
    Type of Medium: Online Resource
    ISSN: 0021-8812 , 1525-3163
    URL: Article
    DOI: 10.1093/jas/skab235.864
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1490550-4
    SSG: 12
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  • 10
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    Protective Effect of Campbell early Leaf extract on Cardiovascular Dysfunction in Spontaneously Hypertensive Rats
    Wiley ; 2021
    In:  The FASEB Journal Vol. 35, No. S1 ( 2021-05)
    Details
    In: The FASEB Journal, Wiley, Vol. 35, No. S1 ( 2021-05)
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v35.S1
    DOI: 10.1096/fasebj.2021.35.S1.02250
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1468876-1
    SSG: 12
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