In:
Journal of Biomedical Materials Research Part A, Wiley, Vol. 94A, No. 4 ( 2010-09-15), p. 1187-1194
Abstract:
This study examined the bone regeneration potential of a novel hybrid membrane consisting of collagen and nano‐bioactive glass (nBG) incorporating basic fibroblast growth factor (FGF2) for use in guided bone regeneration. nBG was added to a reconstitution of collagen at a concentration of 30%, and the hybrid was formulated into a thin membrane. FGF2 (50 μg/ml) was adsorbed to the hybrid membrane. This level of FGF2 was found to be the optimal concentration to stimulate osteoblastic differentiation in vitro . Three membrane groups, including pure collagen, collagen‐nBG hybrid and its combination with FGF2 were implanted within a rat calvarium defect (ϕ = 5 mm) for a period of 3 weeks. Histomorphometric analysis was carried out to evaluate the bone regeneration within the defect. The results showed that the defect in the collagen‐nBG‐FGF2 membrane was recovered almost completely, while partial recovery was observed in the other membrane groups (collagen and collagen‐BG). However, there was little defect recovery in the blank control. The new bone formation was as high as ∼60, ∼45, and ∼30% of the defect treated with the collagen‐nBG‐FGF2, collagen‐BG, and collagen, respectively, whilst only 4% of new bone was observed in the blank control. Overall, the nBG was shown to stimulate bone formation of the collagen membrane, and FGF2 synergistically accelerated the bone regeneration within a rat calvarium defect. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Type of Medium:
Online Resource
ISSN:
1549-3296
,
1552-4965
DOI:
10.1002/jbm.a.v94a:4
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1477192-5
SSG:
12
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