In:
Reproduction, Fertility and Development, CSIRO Publishing, Vol. 12, No. 4 ( 2000), p. 133-
Abstract:
The development of porcine oocytes from large (3.1–8.0 mm in diameter)
or small ( 〈 3.1 mm) follicles was examined after maturation culture in
medium containing porcine follicular fluid (pFF). Large follicles yielded larger (256 m v. 221 m; P 〈 0.05)
cumulus–oocyte complexes and more (22 v. 14%) morphologically normal oocytes than small follicles (Experiment
1). In Experiments 2–4, maturation media supplemented with mixed pFF (10%) from small and large follicles was used. More oocytes from large
follicles matured (58% v. 91%), formed pronuclei (81% v. 90%) and developed to
the blastocyst stage (2% v. 10%) than oocytes from small follicles. In Experiments 5–7, the effects of pFF
collected from either small or large follicles on oocyte development were examined. Regardless of the source of oocytes, large-follicle-derived pFF more
significantly enhanced preimplantation development than did small-follicle-derived pFF. The highest rate of blastocyst formation
(16%) was found when oocytes from large follicles were cultured in maturation medium containing large-follicle-derived pFF. These results suggest
that oocytes from large follicles have greater developmental potential than oocytes from small follicles, and that the origin of pFF, which is added to
the maturation media, might be an important factor for improving in vitro development of porcine oocytes.
Type of Medium:
Online Resource
ISSN:
1031-3613
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
2000
SSG:
12
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