In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_4 ( 2014-04-01), p. 1127-1133
Abstract:
A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11 T , was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11 T grew at 15–40 °C (optimum at 28 °C), at pH 5.0–8.0 (optimum at pH 7.5) and with 0–5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were C 18 : 1 ω7 c , C 16 : 0 , C 18 : 1 2-OH and C 18 : 0 . The genomic DNA G+C content of strain YW11 T was 69.6±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11 T was a member of the genus Roseomonas , and showed the highest sequence similarity to Roseomonas cervicalis KACC 11686 T (97.9 %) and Roseomonas aestuarii KACC 19645 T (97.8 %) and then to Roseomonas ludipueritiae KACC 13843 T (96.9 %). Strain YW11 T showed low DNA–DNA relatedness with R. cervicalis KACC 11686 T (32.3±2.9 %), R. aestuarii KACC 16549 T (28.2±2.6 %) and R. ludipueritiae KACC 13843 T (30.2±2.6 %). Based on the results of phylogenetic analysis and DNA–DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11 T was clearly distinguished from all recognized species of the genus Roseomonas and should be assigned to a novel species of the genus Roseomonas , for which the name Roseomonas rhizosphaerae sp. nov. is proposed. The type strain is YW11 T ( = KACC 17225 T = CCTCC AB2013041 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.057000-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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