In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 4 ( 2020-04-01), p. 2493-2498
Abstract:
A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49 T , was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai–Tibet Plateau, PR China. Analysis of the 16S rRNA gene sequence showed that strain CF-49 T was closely related to Vagococcus elongatus CCUG 51432 T (96.5 % similarity), Vagococcus fluvialis NCFB 2497 T (96.0 %) and Vagococcus lutrae CCUG 39187 T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %. Strains CF-49 T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl. Acid was produced from N -acetylglucosamine, cellobiose, d -fructose, d -glucose, d -mannose, d -mannitol, maltose, d -ribose and salicin. The cell-wall peptidoglycan type was A4α ( l -Lys –d -Asp). The major cellular fatty acids ( 〉 10 %) were C 16 : 0 (35.6 %), C 14 : 0 (17.3 %), C 18 : 1 ω9 c (16.2 %) and C 16 : 1 ω9 c (10.6 %). The predominant respiratory quinone was menaquinone MK-7 (68.8 %). The G+C content of the genomic DNA was 35.9 mol%. Digital DNA–DNA hybridization of strain CF-49 T with V. fluvialis DSM 5731 T , V. elongatus CCUG 51432 T and V. lutrae CCUG 39187 T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively. Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus , with the name Vagococcus xieshaowenii . The type strain is CF-49 T (=CGMCC 1.6436 T =GDMCC 1.1588 T =JCM 33477 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004061
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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