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  • 1
    In: Ecological Applications, Wiley, Vol. 28, No. 3 ( 2018-04), p. 749-760
    Abstract: The biodiversity and high productivity of coastal terrestrial and aquatic habitats are the foundation for important benefits to human societies around the world. These globally distributed habitats need frequent and broad systematic assessments, but field surveys only cover a small fraction of these areas. Satellite‐based sensors can repeatedly record the visible and near‐infrared reflectance spectra that contain the absorption, scattering, and fluorescence signatures of functional phytoplankton groups, colored dissolved matter, and particulate matter near the surface ocean, and of biologically structured habitats (floating and emergent vegetation, benthic habitats like coral, seagrass, and algae). These measures can be incorporated into Essential Biodiversity Variables ( EBV s), including the distribution, abundance, and traits of groups of species populations, and used to evaluate habitat fragmentation. However, current and planned satellites are not designed to observe the EBV s that change rapidly with extreme tides, salinity, temperatures, storms, pollution, or physical habitat destruction over scales relevant to human activity. Making these observations requires a new generation of satellite sensors able to sample with these combined characteristics: (1) spatial resolution on the order of 30 to 100‐m pixels or smaller; (2) spectral resolution on the order of 5 nm in the visible and 10 nm in the short‐wave infrared spectrum (or at least two or more bands at 1,030, 1,240, 1,630, 2,125, and/or 2,260 nm) for atmospheric correction and aquatic and vegetation assessments; (3) radiometric quality with signal to noise ratios ( SNR ) above 800 (relative to signal levels typical of the open ocean), 14‐bit digitization, absolute radiometric calibration 〈 2%, relative calibration of 0.2%, polarization sensitivity 〈 1%, high radiometric stability and linearity, and operations designed to minimize sunglint; and (4) temporal resolution of hours to days. We refer to these combined specifications as H4 imaging. Enabling H4 imaging is vital for the conservation and management of global biodiversity and ecosystem services, including food provisioning and water security. An agile satellite in a 3‐d repeat low‐Earth orbit could sample 30‐km swath images of several hundred coastal habitats daily. Nine H4 satellites would provide weekly coverage of global coastal zones. Such satellite constellations are now feasible and are used in various applications.
    Type of Medium: Online Resource
    ISSN: 1051-0761 , 1939-5582
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
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  • 2
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2010
    In:  Nature Vol. 466, No. 7306 ( 2010-7), p. 569-571
    In: Nature, Springer Science and Business Media LLC, Vol. 466, No. 7306 ( 2010-7), p. 569-571
    Type of Medium: Online Resource
    ISSN: 0028-0836 , 1476-4687
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    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2010
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    detail.hit.zdb_id: 1413423-8
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  • 3
    In: Biochemical Journal, Portland Press Ltd., Vol. 368, No. 2 ( 2002-12-01), p. 397-404
    Abstract: Activation of protein kinase A (cAMP-dependent protein kinase; PKA) triggers insulin secretion in the β-cell. Adenylate cyclase toxin (ACT), a bacterial exotoxin with adenylate cyclase activity, and forskolin, an activator of adenylate cyclase, both dose-dependently increased insulin secretion in the presence, but not the absence, of glucose in insulin-secreting βTC3 cells. The stimulation of cAMP release by either agent was dose-dependent but glucose-independent. Omission of extracellular Ca2+ totally abolished the effects of ACT on insulin secretion and cytosolic cAMP accumulation. ACT and forskolin caused rapid and dramatic increases in cytosolic Ca2+, which were blocked by nifedipine and the omission of extracellular Ca2+. Omission of glucose completely blocked the effects of forskolin and partially blocked the effects of ACT on cytosolic Ca2+. PKA α, β and γ catalytic subunits (Cα, Cβ and Cγ respectively) were identified in βTC6 cells by confocal microscopy. Glucose and glucagon-like polypeptide-1 (GLP-1) caused translocation of Cα to the nucleus and of Cβ to the plasma membrane and the nucleus, but did not affect the distribution of Cγ. In conclusion, glucose and GLP-1 amplify insulin secretion via cAMP production and PKAβ activation.
    Type of Medium: Online Resource
    ISSN: 0264-6021 , 1470-8728
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    Language: English
    Publisher: Portland Press Ltd.
    Publication Date: 2002
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  • 4
    In: Marine and Coastal Fisheries, Wiley, Vol. 8, No. 1 ( 2016-01), p. 502-521
    Abstract: Nearshore marine habitats are productive and vulnerable owing to their connections to pelagic and terrestrial landscapes. To understand how ocean basin‐ and local‐scale conditions may influence nearshore species, we developed an annual index of nearshore production (spanning the period 1972–2010) from growth increments recorded in otoliths of representative pelagic‐feeding (Black Rockfish Sebastes melanops ) and benthic‐feeding (Kelp Greenling Hexagrammos decagrammus ) nearshore‐resident fishes at nine sites in the California Current and Alaska Coastal Current systems. We explored the influence of basin‐ and local‐scale conditions across all seasons at lags of up to 2 years to represent changes in prey quantity (1‐ or 2‐year time lags) and quality (within‐year relationships). Relationships linking fish growth to basin‐scale (Pacific Decadal Oscillation, North Pacific Gyre Oscillation, and multivariate El Niño–Southern Oscillation index) and local‐scale (sea surface temperature, sea surface height anomalies, upwelling index, photosynthetically active radiation, and freshwater discharge) environmental conditions varied by species and current system. Growth of Black Rockfish increased with cool basin‐scale conditions in the California Current and warm local‐scale conditions in the Alaska Coastal Current, consistent with existing hypotheses linking climate to pelagic production on continental shelves in the respective regions. Relationships for Kelp Greenlings in the California Current were complex, with faster growth related to within‐year warm conditions and lagged‐year cool conditions. These opposing, lag‐dependent relationships may reflect differences in conditions that promote quantity versus quality of benthic invertebrate prey in the California Current. Thus, we hypothesize that benthic production is maximized by alternating cool and warm years, as benthic invertebrate recruitment is food limited during warm years while growth is temperature limited by cool years in the California Current. On the other hand, Kelp Greenlings grew faster during and subsequent to warm conditions at basin and local scales in the Alaska Coastal Current. Received November 3, 2015; accepted May 5, 2016
    Type of Medium: Online Resource
    ISSN: 1942-5120 , 1942-5120
    Language: English
    Publisher: Wiley
    Publication Date: 2016
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  • 5
    In: Marine Biology, Springer Science and Business Media LLC, Vol. 162, No. 5 ( 2015-5), p. 1019-1031
    Type of Medium: Online Resource
    ISSN: 0025-3162 , 1432-1793
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 1117-4
    detail.hit.zdb_id: 1459413-4
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  • 6
    Online Resource
    Online Resource
    Wiley ; 1998
    In:  European Journal of Neuroscience Vol. 10, No. 7 ( 1998-07), p. 2416-2425
    In: European Journal of Neuroscience, Wiley, Vol. 10, No. 7 ( 1998-07), p. 2416-2425
    Abstract: We have studied Ca 2+ homeostasis in a unique model of human neurons, the NT2N cell, which differentiates from a human teratocarcinoma cell line, NTera2/C1.D1 by retinoic acid treatment. When perifused with Krebs–HEPES buffer containing 2.5 m m CaCl 2 , fura‐2 loaded NT2N cells produced spontaneous cytosolic Ca 2+ oscillations, or Ca 2+ transients. These cytosolic Ca 2+ transients were not blocked by antagonists of glutamate (6‐cyano‐7‐nitroquinoxaline‐2,3‐dione and d (–)‐2‐amino‐5‐phosphonopentanoic acid) or muscarinic (atropine) receptors. Omission of extracellular Ca 2+ completely abolished Ca 2+ oscillations and decreased the average Ca 2+ level from 106 ± 14 n m to 59 ± 8 n m . Addition of the L‐type Ca 2+ channel blocker nifedipine (1 or 10 μ m ) or of the N‐type inhibitor ω‐conotoxin GVIA (5 μ m ) significantly, although incompletely, suppressed Ca 2+ oscillations, while ω‐conotoxin MVIIC (5 μ m ), a selective antagonist of P‐ and Q‐channels, had no effect. Ni 2+ , at 100 μ m , a concentration selective for T‐type channels, did not inhibit Ca 2+ transients. Non‐specific blockage of Ca 2+ channels by higher concentrations of Ni 2+ (2–5 m m ) or Co 2+ (1 m m ) abolished Ca 2+ oscillations completely. The endoplasmic reticulum Ca 2+ ‐ATPase inhibitor, thapsigargin (1 μ m ), slightly decreased Ca 2+ oscillation frequency, and induced a small transitory increase in the average cytosolic Ca 2+ concentration. The mRNAs of L‐ (α 1D subunit) and N‐type (α 1B subunit) Ca 2+ channel were present in NT2N cells, while that of a T‐type Ca 2+ channel (α 1 ‐subunit) was not present in the NT2N cells as shown by reverse transcription–polymerase chain reaction. In conclusion, NT2N neuronal cells generate cytosolic Ca 2+ oscillations mainly by influx of extracellular Ca 2+ through multiple channels, which include L‐ and N‐type channels, and do not require activation of glutamate or muscarinic receptors.
    Type of Medium: Online Resource
    ISSN: 0953-816X , 1460-9568
    Language: English
    Publisher: Wiley
    Publication Date: 1998
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  • 7
    Online Resource
    Online Resource
    Elsevier BV ; 1998
    In:  Biochemical and Biophysical Research Communications Vol. 247, No. 2 ( 1998-06), p. 407-413
    In: Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 247, No. 2 ( 1998-06), p. 407-413
    Type of Medium: Online Resource
    ISSN: 0006-291X
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    Language: English
    Publisher: Elsevier BV
    Publication Date: 1998
    detail.hit.zdb_id: 1461396-7
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  • 8
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 113, No. 39 ( 2016-09-27)
    Abstract: Complex physiological and behavioral traits, including neurological and psychiatric disorders, often associate with distributed anatomical variation. This paper introduces a global metric, called morphometricity, as a measure of the anatomical signature of different traits. Morphometricity is defined as the proportion of phenotypic variation that can be explained by macroscopic brain morphology. We estimate morphometricity via a linear mixed-effects model that uses an anatomical similarity matrix computed based on measurements derived from structural brain MRI scans. We examined over 3,800 unique MRI scans from nine large-scale studies to estimate the morphometricity of a range of phenotypes, including clinical diagnoses such as Alzheimer’s disease, and nonclinical traits such as measures of cognition. Our results demonstrate that morphometricity can provide novel insights about the neuroanatomical correlates of a diverse set of traits, revealing associations that might not be detectable through traditional statistical techniques.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2016
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  • 9
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 115, No. 7 ( 2018-02-13), p. 1481-1486
    Abstract: When sample sizes are small, the ability to identify weak (but scientifically interesting) associations between a set of predictors and a response may be enhanced by pooling existing datasets. However, variations in acquisition methods and the distribution of participants or observations between datasets, especially due to the distributional shifts in some predictors, may obfuscate real effects when datasets are combined. We present a rigorous statistical treatment of this problem and identify conditions where we can correct the distributional shift. We also provide an algorithm for the situation where the correction is identifiable. We analyze various properties of the framework for testing model fit, constructing confidence intervals, and evaluating consistency characteristics. Our technical development is motivated by Alzheimer’s disease (AD) studies, and we present empirical results showing that our framework enables harmonizing of protein biomarkers, even when the assays across sites differ. Our contribution may, in part, mitigate a bottleneck that researchers face in clinical research when pooling smaller sized datasets and may offer benefits when the subjects of interest are difficult to recruit or when resources prohibit large single-site studies.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2018
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  • 10
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 115, No. 8 ( 2018-02-20), p. 1697-1706
    Abstract: Alzheimer’s disease (AD) is a leading cause of mortality among the elderly. We performed a whole-genome sequencing study of AD in the Chinese population. In addition to the variants identified in or around the APOE locus (sentinel variant rs73052335, P = 1.44 × 10 −14 ), two common variants, GCH1 (rs72713460, P = 4.36 × 10 −5 ) and KCNJ15 (rs928771, P = 3.60 × 10 −6 ), were identified and further verified for their possible risk effects for AD in three small non-Asian AD cohorts. Genotype–phenotype analysis showed that KCNJ15 variant rs928771 affects the onset age of AD, with earlier disease onset in minor allele carriers. In addition, altered expression level of the KCNJ15 transcript can be observed in the blood of AD subjects. Moreover, the risk variants of GCH1 and KCNJ15 are associated with changes in their transcript levels in specific tissues, as well as changes of plasma biomarkers levels in AD subjects. Importantly, network analysis of hippocampus and blood transcriptome datasets suggests that the risk variants in the APOE , GCH1 , and KCNJ15 loci might exert their functions through their regulatory effects on immune-related pathways. Taking these data together, we identified common variants of GCH1 and KCNJ15 in the Chinese population that contribute to AD risk. These variants may exert their functional effects through the immune system.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
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    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2018
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    detail.hit.zdb_id: 1461794-8
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