In:
Cytometry Part B: Clinical Cytometry, Wiley, Vol. 70B, No. 3 ( 2006-05), p. 189-196
Abstract:
JC‐1 probe has been successfully used for the analysis of either apoptosis or P‐glycoprotein (P‐gp) activity. Therefore, we wanted to see if JC‐1 could also simultaneously assess both, P‐gp activity and apoptosis, in acute myeloid leukemia (AML) cells. Methods P‐gp activity was measured using JC‐1 and compared to the results of the Rhodamine 123 (Rh 123) assay in P‐gp negative and P‐gp positive cell lines, and 12 AML samples. For apoptosis, spontaneous apoptosis, as well as, apoptosis induced by Cytosine Arabinosine and Homoharringtonine were analyzed. Both mitochondrial red fluorescence and cytoplasmic green fluorescence of JC‐1 with and without a P‐gp inhibitor (Cyclosporine A : CsA) were used for the identification of apoptotic cells, and this was compared to Annexin V/PI staining. Results (1) We found a good correlation between JC‐1 and Rh 123 in viable cells. Even in a small population of viable cells, P‐gp positive cells emitting low red fluorescence, gained on red fluorescence after P‐gp inhibition with CsA permitting an evaluation of P‐gp activity. (2) We found a good correlation between the Annexin V/PI staining and JC‐1 ( P 〈 0.0001) in the assessment of apoptotic cells. Most importantly, the apoptotic cells could be distinguished by the loss of red fluorescence and the increase of green fluorescence without any change after P‐gp inhibition with CsA. Conclusions JC‐1 can simultaneously evaluate two important parameters involved in drug resistance in AML cells, P‐gp activity and apoptosis. © 2006 International Society for Analytical Cytology
Type of Medium:
Online Resource
ISSN:
1552-4949
,
1552-4957
DOI:
10.1002/cyto.b.v70b:3
DOI:
10.1002/cyto.b.20100
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2180651-2
SSG:
12
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