In:
Acta Crystallographica Section D Biological Crystallography, International Union of Crystallography (IUCr), Vol. 55, No. 4 ( 1999-04-01), p. 877-879
Abstract:
Periplasmic nitrate reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 contains two molybdopterin guanine dinucleotide cofactors and one [4Fe–4S] cluster as prosthetic groups and catalyzes the conversion of nitrate to nitrite. Crystals of the oxidized form of this enzyme were obtained using PEG as precipitant and belong to space group P 3 1 21 or P 3 2 21, with unit-cell dimensions a = b = 106.3, c = 135.1 Å. There is one monomer of 80 kDa in the asymmetric unit, which corresponds to a Matthews ratio of 2.75 Å 3 Da −1 . Using cryo-cooling procedures and X-rays from a rotating-anode generator, diffraction was observed to beyond 3.0 Å resolution.
Type of Medium:
Online Resource
ISSN:
0907-4449
DOI:
10.1107/S0907444998014735
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
1999
detail.hit.zdb_id:
2968623-4
SSG:
12
SSG:
13
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