In:
ChemBioChem, Wiley, Vol. 25, No. 3 ( 2024-02)
Abstract:
Using myoglobin (Mb) as a model protein, we herein developed a facial approach to modifying the heme active site. A cavity was first generated in the heme distal site by F46 C mutation, and the thiol group of Cys46 was then used for covalently linked to exogenous ligands, 1H‐1,2,4‐triazole‐3‐thiol and 1‐(4‐hydroxyphenyl)‐1H‐pyrrole‐2,5‐dione. The engineered proteins, termed F46C‐triazole Mb and F46C‐phenol Mb, respectively, were characterized by X‐ray crystallography, spectroscopic and stopped‐flow kinetic studies. The results showed that both the heme coordination state and the protein function such as H 2 O 2 activation and peroxidase activity could be efficiently regulated, which suggests that this approach might be generally applied to the design of functional heme proteins.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.202300678
Language:
English
Publisher:
Wiley
Publication Date:
2024
detail.hit.zdb_id:
2020469-3
SSG:
12
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