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  • 1
    Online Resource
    Online Resource
    Initial sequencing and analysis of the human genome
    Springer Science and Business Media LLC ; 2001
    In:  Nature Vol. 409, No. 6822 ( 2001-02-15), p. 860-921
    Details
    In: Nature, Springer Science and Business Media LLC, Vol. 409, No. 6822 ( 2001-02-15), p. 860-921
    Type of Medium: Online Resource
    ISSN: 0028-0836 , 1476-4687
    URL: Article
    DOI: 10.1038/35057062
    RVK:
    TA 1000
    RVK:
    UA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2001
    detail.hit.zdb_id: 120714-3
    detail.hit.zdb_id: 1413423-8
    SSG: 11
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  • 2
    Online Resource
    Online Resource
    Occurrence of a Sequence in Marine Cyanophages Similar to That of T4 g20 and Its Application to PCR-Based Detection and Quantification Techniques
    American Society for Microbiology ; 1998
    In:  Applied and Environmental Microbiology Vol. 64, No. 6 ( 1998-06), p. 2051-2060
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 64, No. 6 ( 1998-06), p. 2051-2060
    Abstract: Viruses are ubiquitous components of marine ecosystems and are known to infect unicellular phycoerythrin-containing cyanobacteria belonging to the genus Synechococcus . A conserved region from the cyanophage genome was identified in three genetically distinct cyanomyoviruses, and a sequence analysis revealed that this region exhibited significant similarity to a gene encoding a capsid assembly protein (gp20) from the enteric coliphage T4. The results of a comparison of gene 20 sequences from three cyanomyoviruses and T4 allowed us to design two degenerate PCR primers, CPS1 and CPS2, which specifically amplified a 165-bp region from the majority of cyanomyoviruses tested. A competitive PCR (cPCR) analysis revealed that cyanomyovirus strains could be accurately enumerated, and it was demonstrated that quantification was log-linear over ca. 3 orders of magnitude. Different calibration curves were obtained for each of the three cyanomyovirus strains tested; consequently, cPCR performed with primers CPS1 and CPS2 could lead to substantial inaccuracies in estimates of phage abundance in natural assemblages. Further sequence analysis of cyanomyovirus gene 20 homologs would be necessary in order to design primers which do not exhibit phage-to-phage variability in priming efficiency. It was demonstrated that PCR products of the correct size could be amplified from seawater samples following 100× concentration and even directly without any prior concentration. Hence, the use of degenerate primers in PCR analyses of cyanophage populations should provide valuable data on the diversity of cyanophages in natural assemblages. Further optimization of procedures may ultimately lead to a sensitive assay which can be used to analyze natural cyanophage populations both quantitatively (by cPCR) and qualitatively following phylogenetic analysis of amplified products.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.64.6.2051-2060.1998
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1998
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Cell-to-Cell Communication Across the Prokaryote-Eukaryote Boundary
    American Association for the Advancement of Science (AAAS) ; 2002
    In:  Science Vol. 298, No. 5596 ( 2002-11-08), p. 1207-1207
    Details
    In: Science, American Association for the Advancement of Science (AAAS), Vol. 298, No. 5596 ( 2002-11-08), p. 1207-1207
    Type of Medium: Online Resource
    ISSN: 0036-8075 , 1095-9203
    URL: Article
    DOI: 10.1126/science.1077075
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2002
    detail.hit.zdb_id: 128410-1
    detail.hit.zdb_id: 2066996-3
    detail.hit.zdb_id: 2060783-0
    SSG: 11
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  • 4
    Online Resource
    Online Resource
    How microbes survive in the open ocean
    American Association for the Advancement of Science (AAAS) ; 2017
    In:  Science Vol. 357, No. 6352 ( 2017-08-18), p. 646-647
    Details
    In: Science, American Association for the Advancement of Science (AAAS), Vol. 357, No. 6352 ( 2017-08-18), p. 646-647
    Abstract: A drop of seawater contains almost a million small cells, including genetically diverse bacteria, archaea, and protists, as well as viruses ( 1 ). Typical microscopic views of this diversity give the impression of a dense environment (see the first figure), but they are preceded by filtration and concentration steps that mask the remarkable distances between individual cells in the ocean, where hundreds of micrometers separate cells that themselves are less than a micrometer in size. Yet despite their microscopic size and relative isolation, marine microbes catalyze chemical transformations at rates that are critical for maintaining Earth's habitability. Viewing the open-ocean microbial world through the interwoven threads of space, time, and diffusion is critical for understanding how microbial interactions shape the biogeochemical cycles of one of the largest habitats on Earth.
    Type of Medium: Online Resource
    ISSN: 0036-8075 , 1095-9203
    URL: Article
    DOI: 10.1126/science.aan5764
    RVK:
    TA 1000
    RVK:
    WA 15000
    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2017
    detail.hit.zdb_id: 128410-1
    detail.hit.zdb_id: 2066996-3
    detail.hit.zdb_id: 2060783-0
    SSG: 11
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  • 5
    Online Resource
    Online Resource
    The marine picoplankter Synechococcus sp. WH 7803 exhibits an adaptive response to restricted iron availability
    Oxford University Press (OUP) ; 1996
    In:  FEMS Microbiology Ecology Vol. 21, No. 2 ( 1996-10), p. 69-76
    Details
    In: FEMS Microbiology Ecology, Oxford University Press (OUP), Vol. 21, No. 2 ( 1996-10), p. 69-76
    Type of Medium: Online Resource
    ISSN: 0168-6496 , 1574-6941
    URL: Issue
    URL: Article
    DOI: 10.1111/fem.1996.21.issue-2
    DOI: 10.1111/j.1574-6941.1996.tb00334.x
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1996
    detail.hit.zdb_id: 1501712-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Biogeography of the ubiquitous marine bacterium Alteromonas macleodii determined by multilocus sequence analysis
    Wiley ; 2008
    In:  Molecular Ecology Vol. 17, No. 18 ( 2008-09), p. 4092-4106
    Details
    In: Molecular Ecology, Wiley, Vol. 17, No. 18 ( 2008-09), p. 4092-4106
    Abstract: Twenty‐three isolates of the widely distributed marine bacteria Alteromonas macleodii have been analysed by multilocus sequence analysis combined with phylogenetic and multivariate statistical analyses. The strains originated from the Pacific Ocean, Mediterranean Sea, English Channel, Black Sea and Thailand. Using the nucleotide sequences of nine loci for each of the 23 isolates, a robust identification was achieved of different clades within the single species. Strains generally clustered with the depth in the water column from which the isolate originated. Strains also showed more recombination with isolates from the same vicinity, suggesting that genetic exchange plays a role in diversification of planktonic marine prokaryotes. This study thus shows for the first time for a large set of isolates of a species of planktonic marine prokaryotes that multilocus sequence analysis overcomes the problems associated with the analysis of individual marker genes or presence of extensive recombination events. It can thus achieve intraspecific identification to the level of genotypes and, by comparison with relevant environmental data, ecotypes.
    Type of Medium: Online Resource
    ISSN: 0962-1083 , 1365-294X
    URL: Issue
    URL: Article
    DOI: 10.1111/mec.2008.17.issue-18
    DOI: 10.1111/j.1365-294X.2008.03883.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 2020749-9
    detail.hit.zdb_id: 1126687-9
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Isolation and Molecular Characterization of Five Marine Cyanophages Propagated on Synechococcus sp. Strain WH7803
    American Society for Microbiology ; 1993
    In:  Applied and Environmental Microbiology Vol. 59, No. 11 ( 1993-11), p. 3736-3743
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 59, No. 11 ( 1993-11), p. 3736-3743
    Abstract: Five marine cyanophages propagated on Synechococcus sp. strain WH7803 were isolated from three different oceanographic provinces during the months of August and September 1992: coastal water from the Sargasso Sea, Bermuda; Woods Hole harbor, Woods Hole, Mass.; and coastal water from the English Channel, off Plymouth Sound, United Kingdom. The five cyanophage isolates were found to belong to two families, Myoviridae and Styloviridae, on the basis of their morphology observed in the transmission electron microscope. DNA purified from each of the cyanophage isolates was restricted with a selection of restriction endonucleases, and three distinguishably different patterns were observed. DNA isolated from Myoviridae isolates from Bermuda and the English Channel had highly related restriction patterns, as did DNA isolated from Styloviridae isolates from Bermuda and the English Channel. DNA isolated from the Myoviridae isolate from Woods Hole had a unique restriction pattern. The genome size for each of the Myoviridae isolates was ca. 80 to 85 kb, and it was ca. 90 to 100 kb for each of the Styloviridae isolates. Southern blotting analysis revealed that there was a limited degree of homology among all cyanophage DNAs probed, but clear differences were observed between cyanophage DNA from the Myoviridae and that from the Styloviridae isolates. Polypeptide analysis revealed a clear difference between Myoviridae and Styloviridae polypeptide profiles, although the major, presumably structural, protein in each case was ca. 53 to 54 kDa.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/aem.59.11.3736-3743.1993
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1993
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Methodology for Estimating Numbers of Free-Living and Attached Bacteria in Estuarine Water
    American Society for Microbiology ; 1986
    In:  Applied and Environmental Microbiology Vol. 51, No. 5 ( 1986-05), p. 1110-1120
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 51, No. 5 ( 1986-05), p. 1110-1120
    Abstract: A fundamental problem in estuarine microbiology studies is the accurate determination of the density in the water column of both free-living bacteria and those attached to suspended particulate matter. When a water sample is filtered and the filter is viewed by epifluorescence microscopy, counts can be made of the numbers of bacteria which are seen on the filter background (free-living) and those which appear to lie on sediment particles (both free-living and attached). With only the additional knowledge of the proportion of the filter area covered by particles (a quantity that is straightforwardly determined by stereological point counting), results from geometric probability were used to determine the expected number of bacteria which are hidden by particles and hence to provide an estimation scheme for the true densities of free-living and attached bacteria. Variance equations based on a Taylor series are given, and a partial check of the method is attempted with controlled mixtures of bacteria and sediment. An alternative procedure is also proposed, in which the natural attached/free-living ratio is altered by an intervention experiment, allowing an estimation which is less model dependent but more labor intensive. Both methods are applied to a series of samples from the Tamar estuary, United Kingdom, taken in April 1985. A notable conclusion is that there are always more free-living than attached bacteria in the water column throughout the estuary.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/aem.51.5.1110-1120.1986
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1986
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Cross-kingdom signalling: exploitation of bacterial quorum sensing molecules by the green seaweed Ulva
    The Royal Society ; 2007
    In:  Philosophical Transactions of the Royal Society B: Biological Sciences Vol. 362, No. 1483 ( 2007-07-29), p. 1223-1233
    Details
    In: Philosophical Transactions of the Royal Society B: Biological Sciences, The Royal Society, Vol. 362, No. 1483 ( 2007-07-29), p. 1223-1233
    Abstract: The green seaweed Ulva has been shown to detect signal molecules produced by bacteria. Biofilms that release N -acylhomoserine lactones (AHLs) attract zoospores—the motile reproductive stages of Ulva . The evidence for AHL involvement is based on several independent lines of evidence, including the observation that zoospores are attracted to wild-type bacteria that produce AHLs but are not attracted to mutants that do not produce signal molecules. Synthetic AHL also attracts zoospores and the attraction is lost in the presence of autoinducer inactivation (AiiA) protein. The mechanism of attraction is not chemotactic but involves chemokinesis. When zoospores detect AHLs, the swimming rate is reduced and this results in accumulation of cells at the source of the AHL. It has been demonstrated that the detection of AHLs results in calcium influx into the zoospore. This is the first example of a calcium signalling event in a eukaryote in response to bacterial quorum sensing molecules. The role of AHLs in the ecology of Ulva is discussed. It is probable that AHLs act as cues for the settlement of zoospores, rather than being directly involved as a signalling mechanism.
    Type of Medium: Online Resource
    ISSN: 0962-8436 , 1471-2970
    URL: Article
    DOI: 10.1098/rstb.2007.2047
    RVK:
    WA 15000
    Language: English
    Publisher: The Royal Society
    Publication Date: 2007
    detail.hit.zdb_id: 1462620-2
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Bacterial conversations: talking, listening and eavesdropping. An introduction
    The Royal Society ; 2007
    In:  Philosophical Transactions of the Royal Society B: Biological Sciences Vol. 362, No. 1483 ( 2007-07-29), p. 1115-1117
    Details
    In: Philosophical Transactions of the Royal Society B: Biological Sciences, The Royal Society, Vol. 362, No. 1483 ( 2007-07-29), p. 1115-1117
    Type of Medium: Online Resource
    ISSN: 0962-8436 , 1471-2970
    URL: Article
    DOI: 10.1098/rstb.2007.2038
    RVK:
    WA 15000
    Language: English
    Publisher: The Royal Society
    Publication Date: 2007
    detail.hit.zdb_id: 1462620-2
    SSG: 12
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