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  • 1
    Online Resource
    Online Resource
    Abundance, diversity and functional gene expression of denitrifier communities in adjacent riparian and agricultural zones : Denitrifiers in riparian and agricultural soils
    Oxford University Press (OUP) ; 2011
    In:  FEMS Microbiology Ecology Vol. 77, No. 1 ( 2011-07), p. 69-82
    Details
    In: FEMS Microbiology Ecology, Oxford University Press (OUP), Vol. 77, No. 1 ( 2011-07), p. 69-82
    Type of Medium: Online Resource
    ISSN: 0168-6496
    URL: Issue
    URL: Article
    DOI: 10.1111/fem.2011.77.issue-1
    DOI: 10.1111/j.1574-6941.2011.01084.x
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2011
    detail.hit.zdb_id: 1501712-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Effect of Nitrate and Acetylene on nirS , cnorB , and nosZ Expression and Denitrification Activity in Pseudomonas mandelii
    American Society for Microbiology ; 2009
    In:  Applied and Environmental Microbiology Vol. 75, No. 15 ( 2009-08), p. 5082-5087
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 75, No. 15 ( 2009-08), p. 5082-5087
    Abstract: Nitrate acts as an electron acceptor in the denitrification process. The effect of nitrate in the range of 0 to 1,000 mg/liter on Pseudomonas mandelii nirS , cnorB , and nosZ gene expression was studied, using quantitative reverse transcription-quantitative PCR. Denitrification activity was measured by using the acetylene blockage method and gas chromatography. The effect of acetylene on gene expression was assessed by comparing denitrification gene expression in P. mandelii culture grown in the presence or absence of acetylene. The higher the amount of NO 3 − present, the greater the induction and the longer the denitrification genes remained expressed. nirS gene expression reached a maximum at 2, 4, 4, and 6 h in cultures grown in the presence of 0, 10, 100, and 1,000 mg of KNO 3 /liter, respectively, while induction of nirS gene ranged from 12- to 225-fold compared to time zero. cnorB gene expression also followed a similar trend. nosZ gene expression did not respond to NO 3 − treatment under the conditions tested. Acetylene decreased nosZ gene expression but did not affect nirS or cnorB gene expression. These results showed that nirS and cnorB responded to nitrate concentrations; however, significant denitrification activity was only observed in culture with 1,000 mg of KNO 3 /liter, indicating that there was no relationship between gene expression and denitrification activity under the conditions tested.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00777-09
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2009
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Seasonal changes in the abundance and activity of bacterial and fungal denitrifying communities associated with different compost amendments
    Canadian Science Publishing ; 2022
    In:  Canadian Journal of Microbiology Vol. 68, No. 2 ( 2022-02), p. 91-102
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 68, No. 2 ( 2022-02), p. 91-102
    Abstract: Composts can be efficient organic amendments in potato culture as they can supply carbon and nutrients to the soil. However, more information is required on the effects of composts on denitrification and nitrous oxide emissions (N 2 O) and emission-producing denitrifying communities. The effects of three compost amendments (municipal source separated organic waste compost (SSOC), forestry waste mixed with poultry manure compost (FPMC), and forestry residues compost (FRC)) on fungal and bacterial denitrifying communities and activity was examined in an agricultural field cropped to potatoes during the fall, spring, and summer seasons. The denitrification enzyme activity (DEA), N 2 O emissions, and respiration were measured in parallel. N 2 O emission rates were greater in FRC-amended soils in the fall and summer, whereas soil respiration was highest in the SSOC-amended soil in the fall. A large number of nirK denitrifying fungal transcripts were detected in the fall, coinciding with compost application, while the greatest nirK bacterial transcripts were measured in the summer when plants were actively growing. Denitrifying community and transcript levels were poor predictors of DEA, N 2 O emissions, or respiration rates in compost-amended soil. Overall, the sampling date was driving the population and activity levels of the three denitrifying communities under study.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/cjm-2021-0256
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Production of thaxtomin A by 〈i〉Streptomyces scabies 〈/i〉strains in plant extract containing media
    Canadian Science Publishing ; 1999
    In:  Canadian Journal of Microbiology Vol. 45, No. 9 ( 1999), p. 764-768
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 45, No. 9 ( 1999), p. 764-768
    Type of Medium: Online Resource
    ISSN: 1480-3275 , 0008-4166
    URL: Article
    DOI: 10.1139/cjm-45-9-764
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 1999
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
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  • 5
    Online Resource
    Online Resource
    Changes in Denitrifier Abundance, Denitrification Gene mRNA Levels, Nitrous Oxide Emissions, and Denitrification in Anoxic Soil Microcosms Amended with Glucose and Plant Residues
    American Society for Microbiology ; 2010
    In:  Applied and Environmental Microbiology Vol. 76, No. 7 ( 2010-04), p. 2155-2164
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 76, No. 7 ( 2010-04), p. 2155-2164
    Abstract: In agricultural cropping systems, crop residues are sources of organic carbon (C), an important factor influencing denitrification. The effects of red clover, soybean, and barley plant residues and of glucose on denitrifier abundance, denitrification gene mRNA levels, nitrous oxide (N 2 O) emissions, and denitrification rates were quantified in anoxic soil microcosms for 72 h. nosZ gene abundances and mRNA levels significantly increased in response to all organic carbon treatments over time. In contrast, the abundance and mRNA levels of Pseudomonas mandelii and closely related species ( nirS P ) increased only in glucose-amended soil: the nirS P guild abundance increased 5-fold over the 72-h incubation period ( P 〈 0.001), while the mRNA level significantly increased more than 15-fold at 12 h ( P 〈 0.001) and then subsequently decreased. The nosZ gene abundance was greater in plant residue-amended soil than in glucose-amended soil. Although plant residue carbon-to-nitrogen (C:N) ratios varied from 15:1 to 30:1, nosZ gene and mRNA levels were not significantly different among plant residue treatments, with an average of 3.5 × 10 7 gene copies and 6.9 × 10 7 transcripts g −1 dry soil. Cumulative N 2 O emissions and denitrification rates increased over 72 h in both glucose- and plant-tissue-C-treated soil. The nirS P and nosZ communities responded differently to glucose and plant residue amendments. However, the targeted denitrifier communities responded similarly to the different plant residues under the conditions tested despite changes in the quality of organic C and different C:N ratios.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.02993-09
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2010
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    A phloem-sap feeder mixes phloem and xylem sap to regulate osmotic potential
    Elsevier BV ; 2011
    In:  Journal of Insect Physiology Vol. 57, No. 9 ( 2011-09), p. 1317-1322
    Details
    In: Journal of Insect Physiology, Elsevier BV, Vol. 57, No. 9 ( 2011-09), p. 1317-1322
    Type of Medium: Online Resource
    ISSN: 0022-1910
    URL: Article
    DOI: 10.1016/j.jinsphys.2011.06.007
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2011
    detail.hit.zdb_id: 1482429-2
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Novel P450nor Gene Detection Assay Used To Characterize the Prevalence and Diversity of Soil Fungal Denitrifiers
    American Society for Microbiology ; 2016
    In:  Applied and Environmental Microbiology Vol. 82, No. 15 ( 2016-08), p. 4560-4569
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 82, No. 15 ( 2016-08), p. 4560-4569
    Abstract: Denitrifying fungi produce nitrous oxide (N 2 O), a potent greenhouse gas, as they generally lack the ability to convert N 2 O to dinitrogen. Contrary to the case for bacterial denitrifiers, the prevalence and diversity of denitrifying fungi found in the environment are not well characterized. In this study, denitrifying fungi were isolated from various soil ecosystems, and novel PCR primers targeting the P450nor gene, encoding the enzyme responsible for the conversion of nitric oxide to N 2 O, were developed, validated, and used to study the diversity of cultivable fungal denitrifiers. This PCR assay was also used to detect P450nor genes directly from environmental soil samples. Fungal denitrification capabilities were further validated using an N 2 O gas detection assay and a PCR assay targeting the nirK gene. A collection of 492 facultative anaerobic fungi was isolated from 15 soil ecosystems and taxonomically identified by sequencing the internal transcribed spacer sequence. Twenty-seven fungal denitrifiers belonging to 10 genera had the P450nor and the nirK genes and produced N 2 O from nitrite. N 2 O production is reported in strains not commonly known as denitrifiers, such as Byssochlamys nivea , Volutella ciliata , Chloridium spp., and Trichocladium spp. The prevalence of fungal denitrifiers did not follow a soil ecosystem distribution; however, a higher diversity was observed in compost and agricultural soils. The phylogenetic trees constructed using partial P450nor and nirK gene sequences revealed that both genes clustered taxonomically closely related strains together. IMPORTANCE A PCR assay targeting the P450nor gene involved in fungal denitrification was developed and validated. The newly developed P450nor primers were used on fungal DNA extracted from a collection of fungi isolated from various soil environments and on DNA directly extracted from soil. The results indicated that approximatively 25% of all isolated fungi possessed this gene and were able to convert nitrite to N 2 O. All soil samples from which denitrifying fungi were isolated also tested positive for the presence of P450nor . The P450nor gene detection assay was reliable in detecting a large diversity of fungal denitrifiers. Due to the lack of homology existing between P450nor and bacterial denitrification genes, it is expected that this assay will become a tool of choice for studying fungal denitrifiers.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00231-16
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2016
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Changes in Bacterial Denitrifier Community Abundance over Time in an Agricultural Field and Their Relationship with Denitrification Activity
    American Society for Microbiology ; 2008
    In:  Applied and Environmental Microbiology Vol. 74, No. 19 ( 2008-10), p. 5997-6005
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 74, No. 19 ( 2008-10), p. 5997-6005
    Abstract: This study measured total bacterial and denitrifier community abundances over time in an agricultural soil cropped to potatoes ( Solanum tuberosum L.) by using quantitative PCR. Samples were collected on 10 dates from spring to autumn and from three spatial locations: in the potato “hill” between plants (H), close to the plant (H p ), and in the “furrow” (F). The denitrification rates, N 2 O emissions, and environmental parameters were also measured. Changes in denitrifier abundance over time and spatial location were small (1.7- to 2.7-fold for the nirK , nosZ , and cnorB B guilds), whereas the cnorB P community ( Pseudomonas mandelii and closely related spp.) showed an ∼4.6-fold change. The seasonal patterns of denitrifier gene numbers varied with the specific community: lower nosZ gene numbers in April and May than in June and July, higher cnorB P gene numbers in May and June than in March and April and September and November, higher nirK gene numbers in early spring than in late autumn, and no change in cnorB B gene numbers. Gene numbers were higher for the H p than the H location for the nosZ and nirK communities and for the cnorB P community on individual dates, presumably indicating an effect of the plant on denitrifier abundance. Higher cnorB P gene numbers for the H location than the F location and for nosZ and cnorB B on individual dates reflect the effect of spatial location on abundance. Denitrifier abundance changes were not related to any environmental parameter, although a weak relationship exists between cnorB P gene numbers, extractable organic carbon values, and temperature. Denitrification and N 2 O emissions were mostly regulated by inorganic nitrogen availability and water-filled pore space but were uncoupled from denitrifier community abundances measured in this system.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.00441-08
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2008
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
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  • 9
    Online Resource
    Online Resource
    Streptomycete spores entrapped in chitosan beads as a novel biocontrol tool against common scab of potato
    Springer Science and Business Media LLC ; 2005
    In:  Applied Microbiology and Biotechnology Vol. 68, No. 1 ( 2005-7), p. 104-110
    Details
    In: Applied Microbiology and Biotechnology, Springer Science and Business Media LLC, Vol. 68, No. 1 ( 2005-7), p. 104-110
    Type of Medium: Online Resource
    ISSN: 0175-7598 , 1432-0614
    URL: Article
    DOI: 10.1007/s00253-004-1843-8
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2005
    detail.hit.zdb_id: 1464336-4
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Identification of low-temperature-regulated genes in the fire blight pathogen Erwinia amylovora
    Canadian Science Publishing ; 2006
    In:  Canadian Journal of Microbiology Vol. 52, No. 5 ( 2006-05-01), p. 468-475
    Details
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 52, No. 5 ( 2006-05-01), p. 468-475
    Abstract: Genes involved in pathogenicity of several plant pathogens were shown to be induced at relatively cold temperatures. Loci from the fire blight pathogen Erwinia amylovora (Burrill) induced at 18 °C were identified using the miniTn5 transposon that contains the promoterless reporter gene gusA coding for β-glucuronidase (GUS). Certain mutants (2.7%) expressed GUS predominantly at 18 °C on minimal medium plates, indicating that the transposon had been inserted downstream of a putatively thermoregulated promoter. Those mutants were further screened with a quantitative GUS fluorometric assay. A total of 21 mutants were selected: 19 mutants had a transposon insertion in temperature-dependent genetic loci, with a 2.2- to 6.3-fold induction of gusA gene expression at 18 °C, and two mutants with impaired growth at 18 °C. Some of these genetic loci encoded (i) proteins implicated in flagella biosynthesis, biotin biosynthesis, multi-drug efflux, and type II secretion protein, and (ii) proteins of unknown function.Key words: fire blight, Erwinia amylovora, transposon mutagenesis, gene regulation, low temperature.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    URL: Article
    DOI: 10.1139/w05-153
    RVK:
    WA 15000
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2006
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
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