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  • Wiley  (13)
  • Biodiversity Research  (13)
  • 1
    Online Resource
    Online Resource
    Assessment of Contact Toxicity and Repellent Effects of Essential Oils from Piper Plants Piper yunnanense and Piper boehmeriifolium against Three Stored‐Product Insects
    Wiley ; 2023
    In:  Chemistry & Biodiversity Vol. 20, No. 12 ( 2023-12)
    Details
    In: Chemistry & Biodiversity, Wiley, Vol. 20, No. 12 ( 2023-12)
    Abstract: Storage is a crucial part during grain production for the massive spoilage caused by stored product insects. Essential oils (EOs) of plant origin have been highly recommended to combating insects which are biodegradable and safe mode of action. Hence, to make the fullest use of natural resources, essential oils of different parts from Piper yunnanense (the whole part, PYW; fruits, PYF; leaves, PYL) and Piper boehmeriifolium (leaves, PBL) were extracted by steam distillation method in the present study. Gas chromatography‐mass spectrometry (GC‐MS) characterization revealed bicyclogermacrene (PYW), γ‐muurolene (PYF), δ‐cadinene (PYL) and methyl 4,7,10,13,16,19‐docosahexaenoate (PBL) as the principal compound of each essential oil. Sesquiterpene hydrocarbons were also recognized as the richest class accounting for 56.3 %–94.9 % of the total oil. Three storage pests, Tribolium castaneum , Lasioderma serricorne and Liposceis bostrychophila , were exposed to different concentrations of EOs to determine their insecticidal effects. All tested samples performed modest contact toxicity in contrast to a bioactive ingredient pyrethrin, among which the most substantial effects were observed in PYF EOs against T. castaneum (35.84 μg/adult), PBL EOs against L. serricorne (15.76 μg/adult) and PYW EOs against L. bostrychophila (57.70 μg/cm 2 ). In terms of repellency tests, essential oils of PYF at 78.63 nL/cm 2 demonstrated to have a remarkable repellence against T. castaneum at 2h and 4h post‐exposure. The investigations indicate diverse variations in the chemical profiles and insecticidal efficacies of P. yunnanense and P. boehmeriifolium EOs, providing more experimental evidence for the use of the Piper plants.
    Type of Medium: Online Resource
    ISSN: 1612-1872 , 1612-1880
    URL: Issue
    URL: Article
    DOI: 10.1002/cbdv.v20.12
    DOI: 10.1002/cbdv.202301206
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2139001-0
    SSG: 12
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  • 2
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    The KNRL nuclear receptor controls hydrolase‐mediated vitellin breakdown during embryogenesis in the brown planthopper, Nilaparvata lugens
    Wiley ; 2021
    In:  Insect Science Vol. 28, No. 6 ( 2021-12), p. 1633-1650
    Details
    In: Insect Science, Wiley, Vol. 28, No. 6 ( 2021-12), p. 1633-1650
    Abstract: Vitellin (Vn) homeostasis is central to the fecundity of oviparous insects. Most studies have focused on the synthesis and transportation of Vn as a building block for developing eggs during vitellogenesis; however, less is known about how the utilization of this nutrient reserve affects embryonic development. Here, we show that the single ortholog of the knirps and knirps‐like nuclear receptors, KNRL, negatively regulates Vn breakdown by suppressing the expression of hydrolase genes in the brown planthopper, Nilaparvata lugens . KNRL was highly expressed in the ovary of adult females, and knockdown of KNRL by RNA interference resulted in the acceleration of Vn breakdown and the inhibition of embryonic development. Transcriptome sequencing analysis revealed that numerous hydrolase genes, including cathepsins and trypsins were up‐regulated after KNRL knockdown. At least eight of the nine significantly enriched Gene Ontology terms for the up‐regulated genes were in proteolysis‐related categories. The expression levels of five selected trypsin genes and the enzymatic activities of trypsin in the embryos were significantly increased after KNRL knockdown. Moreover, trypsin injection prolonged egg duration, delayed embryonic development, accelerated Vn breakdown and severely reduced egg hatchability, a pattern similar to that observed in KNRL ‐silenced N. lugens . These observations suggest that KNRL controls Vn breakdown in embryos via the transcriptional inhibition of hydrolases. Generally, this study provides a foundation for understanding how embryo nutrient reserves are mobilized during embryogenesis and identifies several genes and pathways that may prove valuable targets for pest control.
    Type of Medium: Online Resource
    ISSN: 1672-9609 , 1744-7917
    URL: Issue
    URL: Article
    DOI: 10.1111/ins.v28.6
    DOI: 10.1111/1744-7917.12885
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2179775-4
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    The origin and dispersal of Firmiana danxiaensis among isolated specific landscapes
    Wiley ; 2023
    In:  Journal of Systematics and Evolution
    Details
    In: Journal of Systematics and Evolution, Wiley
    Abstract: China has the most numerous Danxia and Karst landscapes, which serve as special terrestrial islands harboring ample endemic species, though how did these endemic species spread among those isolated sites is still an unresolved issue. To address this question, we explored the phylogeographical structure and demographic history of Firmiana danxiaensis , a tree species endemic to Danxia and Karst landscapes. We collected 295 samples (28 populations) of F. danxiaensis . Plastid genomes were assembled for 25 representative samples. Sanger sequencing of four plastid regions and restriction‐site‐associated DNA sequencing were performed on the 28 populations. The phylogenetic tree constructed from plastid genomes and restriction site‐associated DNA sequencing (RAD‐seq) data supported that F. danxiaensis originated from Mount Danxia and Nanxiong Basin, spread to Karst landscapes near Yingde City, and then back to Danxia Mountain and the Nanxiong Basin. In the Nanxiong Basin, the latter arrivals captured the plastid of the former. Population analyses revealed strong population structure among and within Danxia and Karst landscapes, possibly due to low seed and pollen dispersal abilities of the species. The demographic and ecological niche modeling approaches suggested that F. danxiaensis have widely occurred in the southeast of China during the last glacial period, and later retreated to the cliffs of Danxia and Karst landscapes due to temperature rising and competition failure. The declining of the effective population size of the species throughout the postglacial period suggested that global warming, agriculture, and industrial civilizations could have affected the survival of this species, and more measures should be taken to conserve these species.
    Type of Medium: Online Resource
    ISSN: 1674-4918 , 1759-6831
    URL: Article
    DOI: 10.1111/jse.12954
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2516638-4
    SSG: 6,25
    SSG: 12
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  • 4
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    Online Resource
    Proteogenomic analysis reveals alternative splicing and translation as part of the abscisic acid response in Arabidopsis seedlings
    Wiley ; 2017
    In:  The Plant Journal Vol. 91, No. 3 ( 2017-08), p. 518-533
    Details
    In: The Plant Journal, Wiley, Vol. 91, No. 3 ( 2017-08), p. 518-533
    Abstract: In this study, a customized analytical pipeline was developed to study transcriptional and translational changes during the abscisic acid response in plants. Using single molecule long‐read sequencing and short‐read RNA sequencing, we identified numerous alternative spliced (AS) transcripts in Arabidopsis and characterized two new AS types. Proteomic identification indicates differentially expressed AS events were more likely to undergo protein translation. The entire workflow is applicable for other plant species.
    Type of Medium: Online Resource
    ISSN: 0960-7412 , 1365-313X
    URL: Issue
    URL: Article
    DOI: 10.1111/tpj.2017.91.issue-3
    DOI: 10.1111/tpj.13571
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2020961-7
    SSG: 12
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  • 5
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    Online Resource
    Development and characterization of polymorphic microsatellite markers in Castanopsis sclerophylla (Fagaceae)
    Wiley ; 2011
    In:  American Journal of Botany Vol. 98, No. 2 ( 2011-02)
    Details
    In: American Journal of Botany, Wiley, Vol. 98, No. 2 ( 2011-02)
    Abstract: • Premise of the study: We developed polymorphic microsatellite primers in Castanopsis sclerophylla (Lindley & Paxton) Schottky (Fagaceae), a dominant canopy tree, to provide markers for further studies on the genetic structure and mating system of this species. • Methods and Results: Ten polymorphic microsatellite loci were isolated and successfully amplified in three C. sclerophylla populations (Huangshanjian, Shilin, and Guanmiao) from Chun'an, Zhejiang Province, China. The number of alleles per locus in these populations ranged from 3 to 17. The observed and expected heterozygosities were 0.100–0.977 and 0.294–0.916, respectively. • Conclusions: These microsatellite loci displayed moderate or high levels of polymorphism within the examined populations, showing the utility of primers in studying the genetic variation, parentage, and mating system of C. sclerophylla .
    Type of Medium: Online Resource
    ISSN: 0002-9122 , 1537-2197
    URL: Article
    DOI: 10.3732/ajb.1000400
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2053581-8
    SSG: 12
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  • 6
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    Quantum pBac : An effective, high‐capacity piggyBac ‐ based gene integration vector system for unlocking gene therapy potential
    Wiley ; 2023
    In:  The FASEB Journal Vol. 37, No. 9 ( 2023-09)
    Details
    In: The FASEB Journal, Wiley, Vol. 37, No. 9 ( 2023-09)
    Abstract: Recent advances in gene therapy have brought novel treatment options for cancer. However, the full potential of this approach has yet to be unlocked due to the limited payload capacity of commonly utilized viral vectors. Virus‐free DNA transposons, including piggyBac , have the potential to obviate these shortcomings. In this study, we improved a previously modified piggyBac system with superior transposition efficiency. We demonstrated that the internal domain sequences (IDS) within the 3′ terminal repeat domain of hyperactive piggyBac ( hyPB ) donor vector contain dominant enhancer elements. Plasmid‐free donor vector devoid of IDS was used in conjunction with a helper plasmid expressing Quantum PBase ™ v2 to generate an optimal piggyBac system, Quantum pBac ™ ( qPB ), for use in T cells. qPB outperformed hyPB in CD20/CD19 CAR‐T production in terms of performance as well as yield of the CAR‐T cells produced. Furthermore, qPB also produced CAR‐T cells with lower donor‐associated variabilities compared to lentiviral vector. Importantly, qPB yielded mainly CD8 + CAR‐T SCM cells, and the qPB‐ produced CAR‐T cells effectively eliminated CD20/CD19‐expressing tumor cells both in vitro and in vivo. Our findings confirm qPB as a promising virus‐free vector system with an enhanced payload capacity to incorporate multiple genes. This highly efficient and potentially safe system will be expected to further advance gene therapy applications.
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v37.9
    DOI: 10.1096/fj.202201654R
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1468876-1
    SSG: 12
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  • 7
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    Deriving a Statistically Reliable Abundance Index from Landings Data: An Application to the Taiwanese Coastal Dolphinfish Fishery with a Multispecies Feature
    Wiley ; 2019
    In:  Transactions of the American Fisheries Society Vol. 148, No. 1 ( 2019-01), p. 106-122
    Details
    In: Transactions of the American Fisheries Society, Wiley, Vol. 148, No. 1 ( 2019-01), p. 106-122
    Abstract: Data from coastal fisheries are often incomplete, as these fisheries are usually small in scale, rendering them exempt from logbook submission requirements. The catch of Dolphinfish Coryphaena hippurus by Taiwanese fisheries once ranked second in the world but has dramatically declined to very low levels in recent years. To address this decline, assessment of a Dolphinfish abundance index was necessary. However, due to the small scale of the fisheries, logbook data were not available to calculate CPUE . This study aimed to estimate a statistically reliable index by (1) assigning effort matrices to landings data using coastal surveillance radar data; (2) standardizing the 2001–2015 CPUE s while using four approaches (classifying fishing tactics by multivariate techniques and principal components analysis) to differentiate the fisheries' effort toward catching Dolphinfish from the effort toward other target species; and (3) evaluating performance of the standardization models by using an R 2 estimated by cross‐validation and bootstrap procedures. The approach that used a delta‐generalized additive model with a direct principal components procedure demonstrated the best fit. This study presents an example of deriving a statistically reliable abundance index from the data‐incomplete situations common for coastal fisheries, which allows for follow‐up population dynamics studies. The resulting index for Dolphinfish in the Taiwanese region showed two 7‐year cycles, with a prominent decline in 2015. Reasons for the fluctuation are unknown but may be due to environmental factors, the fast‐growing nature of the fish, and heavy exploitation of the stock by Taiwanese fisheries.
    Type of Medium: Online Resource
    ISSN: 0002-8487 , 1548-8659
    URL: Issue
    URL: Article
    DOI: 10.1002/tafs.2019.148.issue-1
    DOI: 10.1002/tafs.10125
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2192460-0
    SSG: 12
    SSG: 21,3
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  • 8
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    Comparison of chimeric antigen receptor ‐T cell‐mediated cytotoxicity assays with suspension tumor cells using plate‐based image cytometry method
    Wiley ; 2023
    In:  Cytometry Part A Vol. 103, No. 1 ( 2023-01), p. 27-38
    Details
    In: Cytometry Part A, Wiley, Vol. 103, No. 1 ( 2023-01), p. 27-38
    Abstract: In the recent decade, chimeric antigen receptor (CAR)‐T cell therapy has revolutionized strategies for cancer treatments due to its highly effective clinical efficacy and response for B cell malignancies. The success of CAR‐T cell therapy has stimulated the increase in the research and development of various CAR constructs to target different tumor types. Therefore, a robust and efficient in vitro potency assay is needed to quickly identify potential CAR gene design from a library of construct candidates. Image cytometry methodologies have been utilized for various CAR‐T cell‐mediated cytotoxicity assay using different fluorescent labeling methods, mainly due to their ease‐of‐use, ability to capture cell images for verification, and higher throughput performance. In this work, we employed the Celigo Image Cytometer to evaluate and compare two CAR‐T cell‐mediated cytotoxicity assays using GFP‐expressing or fluorescent dye‐labeled myeloma and plasmacytoma cells. The GFP‐based method demonstrated higher sensitivity in detecting CAR‐T cell‐mediated cytotoxicity when compared to the CMFDA/DAPI viability method. We have established the criteria and considerations for the selection of cytotoxicity assays that are fit‐for‐purpose to ensure the results produced are meaningful for the specific testing conditions.
    Type of Medium: Online Resource
    ISSN: 1552-4922 , 1552-4930
    URL: Issue
    URL: Article
    DOI: 10.1002/cyto.a.v103.1
    DOI: 10.1002/cyto.a.24673
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2180639-1
    SSG: 12
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  • 9
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    Clusterin suppresses spermatogenic cell apoptosis to alleviate diabetes‐induced testicular damage by inhibiting autophagy via the PI3K/AKT/mTOR axis
    Wiley ; 2021
    In:  Biology of the Cell Vol. 113, No. 1 ( 2021-01), p. 14-27
    Details
    In: Biology of the Cell, Wiley, Vol. 113, No. 1 ( 2021-01), p. 14-27
    Abstract: Diabetes‐induced testicular dysfunction is characterised by abnormal apoptosis of spermatogenic cells, but the underlying mechanism is poorly understood. This study aimed to investigate the roles of clusterin (CLU) in testicular damage associated with diabetes pathogenesis, as well as the molecular mechanism. A rat diabetes model was established using streptozocin, and the mouse spermatogenic cell line GC‐1 spg was treated with high glucose as a cellular model. CLU was overexpressed in GC‐1 spg cells, followed by detection of serum testosterone, cell proliferation, cell apoptosis and autophagy. Results CLU expression was significantly reduced and LC3 expression was elevated in testis tissues in the rat diabetes model and high glucose‐treated GC‐1 spg cells. High glucose led to suppressed viability, enhanced apoptosis, reduced Bcl‐2 expression, elevated Bax expression and cleavage of Caspase‐3/‐9 in GC‐1 spg cells, and these effects were abrogated by CLU overexpression. Additionally, CLU overexpression repressed LC3 and Beclin‐1 expression, reduced the LC3II/LC3I ratio and promoted p62 expression in GC‐1 spg cells in the presence of high glucose, and these effects were all mitigated by rapamycin treatment. Inhibition of PI3K/AKT/mTOR signalling with LY294002 activated autophagy in CLU‐overexpressing GC‐1 spg cells under high glucose conditions. CLU overexpression repressed autophagy and alleviated testicular damage in diabetic rats, which was also abrogated by LY294002 treatment. Conclusions CLU expression is suppressed during diabetes‐induced testicular damage, whereas CLU overexpression alleviates diabetes‐induced testicular damage by activating PI3K/AKT/mTOR signalling to inhibit autophagy and further repress spermatogenic cell apoptosis.
    Type of Medium: Online Resource
    ISSN: 0248-4900 , 1768-322X
    URL: Issue
    URL: Article
    DOI: 10.1111/boc.v113.1
    DOI: 10.1111/boc.202000030
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011750-4
    SSG: 12
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  • 10
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    Arabidopsis NPF2 .13 functions as a critical transporter of bacterial natural compound tunicamycin in plant–microbe interaction
    Wiley ; 2023
    In:  New Phytologist Vol. 238, No. 2 ( 2023-04), p. 765-780
    Details
    In: New Phytologist, Wiley, Vol. 238, No. 2 ( 2023-04), p. 765-780
    Abstract: Metabolites including antibiotics, enzymes, and volatiles produced by plant‐associated bacteria are key factors in plant–microbiota interaction that regulates various plant biological processes. There should be crucial mediators responsible for their entry into host plants. However, less is known about the identities of these plant transporters. We report that the Arabidopsis Nitrate Transporter1 (NRT1)/NPF protein NPF2.13 functions in plant uptake of tunicamycin (TM), a natural antibiotic produced by several Streptomyces spp., which inhibits protein N‐glycosylation. Loss of NPF2.13 function resulted in enhanced TM tolerance, whereas NPF2.13 overexpression led to TM hypersensitivity. Transport assays confirmed that NPF2.13 is a H + /TM symporter and the transport is not affected by other substrates like nitrate. NPF2.13 exclusively showed TM transport activity among tested NPFs. Tunicamycin uptake from TM‐producing Streptomyces upregulated the expression of nitrate‐related genes including NPF2.13 . Moreover, nitrate allocation to younger leaves was promoted by TM in host plants. Tunicamycin could also benefit plant defense against the pathogen. Notably, the TM effects were significantly repressed in npf2.13 mutant. Overall, this study identifies NPF2.13 protein as an important TM transporter in plant–microbe interaction and provides insights into multiple facets of NPF proteins in modulating plant nutrition and defense by transporting exterior bacterial metabolites.
    Type of Medium: Online Resource
    ISSN: 0028-646X , 1469-8137
    URL: Issue
    URL: Article
    DOI: 10.1111/nph.v238.2
    DOI: 10.1111/nph.18752
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 208885-X
    detail.hit.zdb_id: 1472194-6
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