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  • Wiley  (18)
  • Biodiversity Research  (18)
  • 1
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    Proteomic analyses of the interaction between the plant‐growth promoting rhizobacterium Paenibacillus polymyxa E681 and Arabidopsis thaliana
    Wiley ; 2016
    In:  PROTEOMICS Vol. 16, No. 1 ( 2016-01), p. 122-135
    Details
    In: PROTEOMICS, Wiley, Vol. 16, No. 1 ( 2016-01), p. 122-135
    Abstract: Plant growth‐promoting rhizobacteria (PGPR) facilitate the plant growth and enhance their induced systemic resistance (ISR) against a variety of environmental stresses. In this study, we carried out integrative analyses on the proteome, transcriptome, and metabolome to investigate Arabidopsis root and shoot responses to the well‐known PGPR strain Paenibacillus polymyxa ( P. polymyxa ) E681. Shoot fresh and root dry weights were increased, whereas root length was decreased by treatment with P. polymyxa E681. 2DE approach in conjunction with MALDI‐TOF/TOF analysis revealed a total of 41 (17 spots in root, 24 spots in shoot) that were differentially expressed in response to P. polymyxa E681. Biological process‐ and molecular function‐based bioinformatics analysis resulted in their classification into seven different protein groups. Of these, 36 proteins including amino acid metabolism, antioxidant, defense and stress response, photosynthesis, and plant hormone‐related proteins were up‐regulated, whereas five proteins including three carbohydrate metabolism‐ and one amino acid metabolism‐related, and one unknown protein were down‐regulated, respectively. A good correlation was observed between protein and transcript abundances for the 12 differentially expressed proteins during interactions as determined by qPCR analysis. Metabolite analysis using LC‐MS/MS revealed highly increased levels of tryptophan, indole‐3‐acetonitrile (IAN), indole‐3‐acetic acid (IAA), and camalexin in the treated plants. Arabidopsis plant inoculated P. polymyxa E681 also showed resistance to Botrytis cinerea infection. Taken together these results suggest that P. polymyxa E681 may promote plant growth by induced metabolism and activation of defense‐related proteins against fungal pathogen.
    Type of Medium: Online Resource
    ISSN: 1615-9853 , 1615-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/pmic.v16.1
    DOI: 10.1002/pmic.201500196
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2037674-1
    detail.hit.zdb_id: 2032093-0
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  • 2
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    Effects of moderate intensity static magnetic fields on human bone marrow‐derived mesenchymal stem cells
    Wiley ; 2015
    In:  Bioelectromagnetics Vol. 36, No. 4 ( 2015-04), p. 267-276
    Details
    In: Bioelectromagnetics, Wiley, Vol. 36, No. 4 ( 2015-04), p. 267-276
    Abstract: This study aimed to explore effects of static magnetic fields (SMFs) of moderate intensity (3–50 mT) as biophysical stimulators of proliferation and osteoblastic differentiation of human bone marrow‐derived mesenchymal stem cells (MSCs). MSCs were exposed to SMFs of three intensities: 3, 15, and 50 mT. Proliferation was assessed by cell counting and bromodeoxyuridine incorporation, and differentiation by measuring alkaline phosphatase (ALP) activity, calcium content, mineralized nodule formation, and transcripts of osteogenic markers. Moderate intensity SMFs increased cell proliferation, ALP activity, calcium release, and mineralized nodule formation in a dose‐ and time‐dependent manner, which peaked at 15 mT. In the same manner, they upregulated expression of osteogenic marker genes such as ALP, bone sialoprotein 2 (BSP2), collagen1a1 (COL1a1), osteocalcin (OCN), osteonectin (ON), osteopontin (OPN), osterix (OSX), and runt‐related transcription factor 2 (RUNX2) with peak at 15 mT after 14 or 21 days of exposure. Results demonstrate that moderate intensity SMFs promote proliferation and osteoblastic differentiation of MSCs. This effect could help to improve MSC responses during osseointegration between a dental implant and surrounding bone. Bioelectromagnetics. 36:267–276, 2015. © 2015 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0197-8462 , 1521-186X
    URL: Issue
    URL: Article
    DOI: 10.1002/bem.v36.4
    DOI: 10.1002/bem.21903
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2001228-7
    detail.hit.zdb_id: 760683-7
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  • 3
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    Proteomic analysis of microvesicles derived from human colorectal cancer ascites
    Wiley ; 2011
    In:  PROTEOMICS Vol. 11, No. 13 ( 2011-07), p. 2745-2751
    Details
    In: PROTEOMICS, Wiley, Vol. 11, No. 13 ( 2011-07), p. 2745-2751
    Abstract: The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites‐derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1‐D SDS‐PAGE and nano‐LC‐MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon‐specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.
    Type of Medium: Online Resource
    ISSN: 1615-9853 , 1615-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/pmic.v11.13
    DOI: 10.1002/pmic.201100022
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2037674-1
    detail.hit.zdb_id: 2032093-0
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  • 4
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    Characteristics of Saccharomyces cerevisiae gal1 Δ and gal1 Δ hxk2 Δ mutants expressing recombinant proteins from the GAL promoter
    Wiley ; 2005
    In:  Biotechnology and Bioengineering Vol. 89, No. 6 ( 2005-03-20), p. 619-629
    Details
    In: Biotechnology and Bioengineering, Wiley, Vol. 89, No. 6 ( 2005-03-20), p. 619-629
    Abstract: Galactose can be used not only as an inducer of the GAL promoters, but also as a carbon source by Saccharomyces cerevisiae , which makes recombinant fermentation processes that use GAL promoters complicated and expensive. To overcome this problem during the cultivation of the recombinant strain expressing human serum albumin (HSA) from the GAL10 promoter, a gal1 Δ mutant strain was constructed and its induction kinetics investigated. As expected, the gal1 Δ strain did not use galactose, and showed high levels of HSA expression, even at extremely low galactose concentrations (0.05–0.1 g/L). However, the gal1 Δ strain produced much more ethanol, in a complex medium containing glucose, than the GAL1 strain. To improve the physiological properties of the gal1 Δ mutant strain as a host for heterologous protein production, a null mutation of either MIG1 or HXK2 was introduced into the gal1 Δ mutant strain, generating gal1 Δ mig1 Δ and gal1 Δ hxk2 Δ double strains. The gal1 Δ hxk2 Δ strain showed a decreased rate of ethanol synthesis, with an accelerated rate of ethanol consumption, compared to the gal1 Δ strain, whereas the gal1 Δ mig1 Δ strain showed similar patterns to the gal1 Δ strain. Furthermore, the gal1 Δ hxk2 Δ strain secreted much more recombinant proteins (HSA and HSA fusion proteins) than the other strains. The results suggest that the gal1 Δ hxk2 Δ strain would be useful for the large‐scale production of heterologous proteins from the GAL10 promoter in S. cerevisiae. © 2005 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0006-3592 , 1097-0290
    URL: Issue
    URL: Article
    DOI: 10.1002/bit.v89:6
    DOI: 10.1002/bit.20240
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 1480809-2
    detail.hit.zdb_id: 280318-5
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  • 5
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    The homeodomain‐leucine zipper ATHB23 , a phytochrome B‐interacting protein, is important for phytochrome B‐mediated red light signaling
    Wiley ; 2014
    In:  Physiologia Plantarum Vol. 150, No. 2 ( 2014-02), p. 308-320
    Details
    In: Physiologia Plantarum, Wiley, Vol. 150, No. 2 ( 2014-02), p. 308-320
    Abstract: Phytochromes are red (R)/far‐red ( FR ) photoreceptors that are central to the regulation of plant growth and development. Although it is well known that photoactivated phytochromes are translocated into the nucleus where they interact with a variety of nuclear proteins and ultimately regulate genome‐wide transcription, the mechanisms by which these photoreceptors function are not completely understood. In an effort to enhance our understanding of phytochrome‐mediated light signaling networks, we attempted to identify novel proteins interacting with phytochrome B ( phyB ). Using affinity purification in Arabidopsis phyB overexpressor, coupled with mass spectrometry analysis, 16 proteins that interact with phyB in vivo were identified. Interactions between phyB and six putative phyB ‐interacting proteins were confirmed by bimolecular fluorescence complementation ( BiFC ) analysis. Involvement of these proteins in phyB ‐mediated signaling pathways was also revealed by physiological analysis of the mutants defective in each phyB ‐interacting protein. We further characterized the athb23 mutant impaired in the homeobox protein 23 ( ATHB23 ) gene. The athb23 mutant displayed altered hypocotyl growth under R light, as well as defects in phyB ‐dependent seed germination and phyB ‐mediated cotyledon expansion. Taken together, these results suggest that the ATHB23 transcription factor is a novel component of the phyB ‐mediated R light signaling pathway.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.2014.150.issue-2
    DOI: 10.1111/ppl.12087
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
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  • 6
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    Modification of dendritic cells with interferon‐γ‐inducible protein‐10 gene to enhance vaccine potency
    Wiley ; 2009
    In:  The Journal of Gene Medicine Vol. 11, No. 10 ( 2009-10), p. 889-898
    Details
    In: The Journal of Gene Medicine, Wiley, Vol. 11, No. 10 ( 2009-10), p. 889-898
    Abstract: Dendritic cell (DC)‐based vaccines have become a promising modality in cancer immunotherapy. However, their ability to initiate tumor antigen‐specific T cell immunity is limited in various negative‐feedback mechanisms. The rapid down‐regulation of chemokines, such as the interferon inducible protein of 10 kDa (IP‐10), which chemoattracts activated antigen‐specific CD8 + T cells, would represent negative‐feedback regulation. Therefore, we attempted to improve DC vaccine potency by introducing the IP‐10 gene retrovirally aiming to replenish the chemoattractive activity of DCs. Methods We introduced IP‐10 gene into DC2.4 cells, referred to as DC‐IP10, using a retroviral system. Nonsecretable mIP‐10‐expressing DCs (DC‐mIP10) were also prepared to evaluate the effects of secretion in IP‐10‐mediated modulation of DC biology. Additionally, in vitro and in vivo activation of antigen‐specific T lymphocytes and in vivo anti‐tumor effects induced by DC‐IP10 or DC‐mIP10 were determined. Results The modification of DC2.4 cells with the IP‐10 gene resulted in the secretion of functionally chemoattractive IP‐10 and, unexpectedly, a significant up‐regulation of surface expression in co‐stimulatory molecules, such as CD40 and CD80, compared to that of DCs with vector control (DC‐no insert). DC‐mIP10 also displayed the partially matured phenotypes but failed to recruit antigen‐specific T cells in an in vitro cell culture system. Consistently, DC‐IP10 generated more tumor antigen‐specific CD8 + T cells and stronger anti‐tumor effects in vaccinated mice than did control DCs and DC‐mIP10. Conclusions The results obtained provide the groundwork for a future clinical translation of the chemokine‐based genetic modification of DCs to increase their vaccine potency. Copyright © 2009 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1099-498X , 1521-2254
    URL: Issue
    URL: Article
    DOI: 10.1002/jgm.v11:10
    DOI: 10.1002/jgm.1371
    Language: English
    Publisher: Wiley
    Publication Date: 2009
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  • 7
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    Prevention of inflammation‐mediated neurotoxicity by butylidenephthalide and its role in microglial activation
    Wiley ; 2013
    In:  Cell Biochemistry and Function Vol. 31, No. 8 ( 2013-12), p. 707-712
    Details
    In: Cell Biochemistry and Function, Wiley, Vol. 31, No. 8 ( 2013-12), p. 707-712
    Abstract: Microglial cells are the prime effectors in immune and inflammatory responses of the central nervous system (CNS). During pathological conditions, the activation of these cells helps restore CNS homeostasis. However, chronic microglial activation endangers neuronal survival through the release of various proinflammatory molecules and neurotoxins. Thus, negative regulators of microglial activation have been considered as potential therapeutic candidates to target neurodegeneration, such as that in Alzheimer's and Parkinson's diseases. The rhizome of Ligusticum chuanxiong Hort. ( Ligusticum wallichii Franch) has been widely used for the treatment of vascular diseases in traditional oriental medicine. Butylidenephthalide (BP), a major bioactive component from L. chuanxiong , has been reported to have a variety of pharmacological activities, including vasorelaxant, anti‐anginal, anti‐platelet and anti‐cancer effects. The aim of this study was to examine whether BP represses microglial activation. In rat brain microglia, BP significantly inhibited the lipopolysaccharide (LPS)‐induced production of nitric oxide (NO), tumour necrosis factor‐ α and interleukin‐1 β . In organotypic hippocampal slice cultures, BP clearly blocked the effect of LPS on hippocampal cell death and inhibited LPS‐induced NO production in culture medium. These results newly suggest that BP provide neuroprotection by reducing the release of various proinflammatory molecules from activated microglia. Copyright © 2013 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0263-6484 , 1099-0844
    URL: Issue
    URL: Article
    DOI: 10.1002/cbf.v31.8
    DOI: 10.1002/cbf.2959
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 283643-9
    detail.hit.zdb_id: 1496553-7
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  • 8
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    Neuroprotective effects of hexane‐ and methylene chloride‐soluble fractions of Salicornia herbacea L. in mouse hippocampal HT22 cells
    Wiley ; 2015
    In:  The FASEB Journal Vol. 29, No. S1 ( 2015-04)
    Details
    In: The FASEB Journal, Wiley, Vol. 29, No. S1 ( 2015-04)
    Abstract: Salicornia herbacea L. is one of the halophytic herbs that grow in salt marshes and on muddy seashores. It has been reported to exhibit their bioactivities including anti‐diabetic, antioxidant and anticancer activities. However, studies on the neuroprotective effect of this plant have not been conducted. Salicornia herbacea L. was extracted with 80 % ethanol, followed by further fractionation into five subfractions according to polarity; n ‐hexane, methylene chloride, ethyl acetate, n ‐butanol and water soluble fractions. The neuroprotective effect of plant extract and its solvent fractions was evaluated by their ability to counteract cell death and ROS generation induced by glutamate in murine hippocampal HT22 cell line. Our results indicated that n ‐hexane and methylene chloride‐soluble fractions had the highest neuroprotective activities among 5 subfractions. These fractions also induced NAD(P)H:quinone oxidoreductase, an antioxidant marker enzyme, in a concentration‐dependent manner. These results warrant further mechanistic study and identification of active compounds in n ‐hexane and methylene chloride‐soluble fractions of Salicornia herbacea L. (Supported by a grant (R0002736) from Regional Industry R & D Assistance Program (2013) of Ministry of Trade, Industry and Energy, Republic of Korea)
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v29.S1
    DOI: 10.1096/fasebj.29.1_supplement.607.12
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1468876-1
    detail.hit.zdb_id: 639186-2
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  • 9
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    Confocal Raman microspectroscopic study of folate receptor‐targeted delivery of 6‐mercaptopurine‐embedded gold nanoparticles in a single cell
    Wiley ; 2012
    In:  Journal of Biomedical Materials Research Part A Vol. 100A, No. 5 ( 2012-05), p. 1221-1228
    Details
    In: Journal of Biomedical Materials Research Part A, Wiley, Vol. 100A, No. 5 ( 2012-05), p. 1221-1228
    Abstract: We investigate the cellular uptake behaviors and efficacy of folate‐coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate‐conjugated AuNPs embedded with a purine analogue cancer drug of 6‐mercaptopurine (6MP) were assembled via a 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4‐aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy. The internalization of the conjugated AuNPs inside the folate receptor‐positive HeLa and KB cells was checked by TEM and dark‐field microscopy (DFM) combined with label‐free confocal spectroscopy over the depth variable z at a micrometer resolution. DFM live cell imaging of folate‐conjugated AuNPs in HeLa cells indicated that the targeted AuNPs appeared to attach on the cell surfaces and enter into the cell with an hour. The cell viability was also compared to estimate the efficacy of folate‐conjugated AuNP delivery systems. Folate receptor‐targeted AuNP systems appeared to decrease cancer cell viability both in vitro and in vivo more than did the use of the 6MP‐coated AuNPs drug without any targeting systems. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
    Type of Medium: Online Resource
    ISSN: 1549-3296 , 1552-4965
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.a.v100a.5
    DOI: 10.1002/jbm.a.33294
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 1477192-5
    detail.hit.zdb_id: 2099989-6
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  • 10
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    Integrated Management of Haricot Bean Foliage Beetle in Northeastern Ethiopia
    Wiley ; 2019
    In:  Psyche: A Journal of Entomology Vol. 2019 ( 2019-06-17), p. 1-5
    Details
    In: Psyche: A Journal of Entomology, Wiley, Vol. 2019 ( 2019-06-17), p. 1-5
    Abstract: A field experiment was conducted to determine the integrated effect of planting dates, insecticides, and their interaction on the reduction of yield and yield related components of haricot bean caused by haricot bean foliage beetle damage at Sirinka Agriculture Research Center, Ethiopia. Planting dates were normal planting (NP) and late planting (10 days after normal planting) (LP), while insecticides comprised Apron star seed dressing (A) and without insecticide (WI). The combined analysis revealed that late planting combined with Apron star seed dressing (LPA) resulted in the highest yield (1223.7 Kg/ha). On the other hand, normal planting date without insecticide application (NPWI) gave the lowest yield (209.6 kg/ha) and the maximum yield loss (209.6%). The cost-benefit analysis showed that use of LPA gave by far better high net profit over control. Thus, LPA are recommended for haricot bean foliage beetle management in northeastern Ethiopia.
    Type of Medium: Online Resource
    ISSN: 0033-2615 , 1687-7438
    URL: Article
    DOI: 10.1155/2019/3728651
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 281541-2
    detail.hit.zdb_id: 2409524-2
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