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  • Wiley  (27)
  • Biodiversity Research  (27)
  • 1
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    Online Resource
    Multi‐particle interaction in AC electric field driven by dielectrophoresis force
    Wiley ; 2021
    In:  ELECTROPHORESIS Vol. 42, No. 21-22 ( 2021-11), p. 2189-2196
    Details
    In: ELECTROPHORESIS, Wiley, Vol. 42, No. 21-22 ( 2021-11), p. 2189-2196
    Abstract: When the dielectrophoresis technology is used to manipulate micron‐sized particles, the interaction between particles should not be ignored because of the particle‐particle interaction. Especially, when multiple particles (number of particles is above 2) are simultaneously manipulated, the interaction between neighboring particles will affect the results of the manipulation. This research investigates the interaction of particles caused dielectrophoresis effect by the Arbitrary Lagrangian‐Eulerian (ALE) method based on the hypothesis of the thin layer of the electric double layer at the microscale. The mathematics model can be solved simultaneously by the finite element method for the AC electric field, the flow field around the suspended particles and the particle mechanics at the micrometer scale. In this study, the particle conductivity and the direction of the electric field are investigated, we find that particle conductivity and electric field direction pose an impact on particle movement, and the research reveal the law of microparticle dielectrophoresis movement, which could offer theoretical and technology support to profoundly understand the precise manipulation of particles in microfluidic chips by the dielectrophoresis effect.
    Type of Medium: Online Resource
    ISSN: 0173-0835 , 1522-2683
    URL: Issue
    URL: Article
    DOI: 10.1002/elps.v42.21-22
    DOI: 10.1002/elps.202100094
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1475486-1
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  • 2
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    Responses to nutrient addition, shading and clipping in a range expanding invasive plant, Mikania micrantha
    Wiley ; 2022
    In:  Weed Research Vol. 62, No. 5 ( 2022-10), p. 372-379
    Details
    In: Weed Research, Wiley, Vol. 62, No. 5 ( 2022-10), p. 372-379
    Abstract: Invasive plants often spread in their new range. During range expansion, the population density at the invasion front may be low compared to that at the invasion centre, and this may select for an acquisitive growth strategy, such as rapid growth, stronger responses to nutrient addition, less tolerance to shading and more tolerance to biomass removal. In this study, we tested this idea using 27 populations of an invasive plant, Mikania micrantha , which is spreading on Hainan island of China in the past 20 years. Nutrient addition increased biomass of M. micrantha , and shading and clipping reduced it. Population density, frequency of occurrence and their interaction with nutrient addition, shading or clipping did not influence plant biomass and root to shoot ratio (RSR). For the western populations, both population density and frequency of occurrence were negatively correlated with RSR in the shading treatment. Overall, populations of M. micrantha differed little in growth. The reasons for these results may be that the spread distance and residence time of M. micrantha on Hainan island are too short to drive any evolutionary change, strong interspecific invaders may prevent M. micrantha from acquiring abundant resources when population density and frequency are low, and long distance dispersal of seeds and pollen may potentially mix genes, preventing adaptive evolution. These results indicate that M. micrantha may not evolve to be more invasive during range expansion. Future studies can examine if populations of M. micrantha differ in other aspects, such as seed dispersal ability and competitive ability.
    Type of Medium: Online Resource
    ISSN: 0043-1737 , 1365-3180
    URL: Issue
    URL: Article
    DOI: 10.1111/wre.v62.5
    DOI: 10.1111/wre.12552
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2016371-X
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  • 3
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    Influence of season and conditions of surrogate sows on efficiency of somatic cell cloning production
    Wiley ; 2023
    In:  Reproduction in Domestic Animals Vol. 58, No. 9 ( 2023-09), p. 1261-1269
    Details
    In: Reproduction in Domestic Animals, Wiley, Vol. 58, No. 9 ( 2023-09), p. 1261-1269
    Abstract: This study was focused on the effects of ovary acquisition season, embryo transfer season, and conditions of surrogate sows on cloning efficiency, with the objective of improving the production of cloned pigs. The statistical analysis documented that cloning efficiency was highest when ovary extraction and embryo transfer occurred in the spring, and lowest when such operations occurred in the autumn. This was evidenced by the higher number of recovered oocytes (3.2 ± 0.47 vs. 2.5 ± 0.51), rate of mature oocytes (57.4 ± 0.07% vs. 48.9 ± 0.06%), rate of developed cloned blastocysts (35.7 ± 0.12% vs. 34.4 ± 0.07%), pregnancy rate of surrogate sows (73.5% vs. 33.3%), delivery rate (67.6% vs. 16.7%), litter size (6.9 ± 2.3 vs. 2.3 ± 2.5), and the number of alive newborns (5.7 ± 2.2 vs. 1.3 ± 1.2). Cloning efficiency was little affected by the ovulatory status of the surrogate sow prior to embryo transfer. The length of pregnancy, the parity, and the length of labor of the surrogate sow significantly affected the efficiency of generating pigs cloned from somatic cells. Specifically, when length of pregnancy ranged from 111 to 117 days, surrogate sows with shorter gestation period had larger litter size (8.9 ± 2.8) and a higher number of stillbirths per litter (2.1 ± 2.0). Moreover, statistical analysis indicated that selecting sows with 2–4 parities as surrogates led to increased litter size (7.7 ± 3.0) and the number of alive newborns (6.4 ± 3.1). In comparison with naturally breeding sows, the surrogate sows spent more time giving birth and suffered higher rates of stillbirth. The data obtained in this study provide valuable insights for improving the production efficiency of somatic cell cloned pigs.
    Type of Medium: Online Resource
    ISSN: 0936-6768 , 1439-0531
    URL: Issue
    URL: Article
    DOI: 10.1111/rda.v58.9
    DOI: 10.1111/rda.14429
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020494-2
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  • 4
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    A serpin ( CvT ‐serpin15) of teratocytes contributes to microbial‐resistance in Plutella xylostella during Cotesia vestalis parasitism
    Wiley ; 2021
    In:  Pest Management Science Vol. 77, No. 10 ( 2021-10), p. 4730-4740
    Details
    In: Pest Management Science, Wiley, Vol. 77, No. 10 ( 2021-10), p. 4730-4740
    Abstract: Parasitic wasps are an important group of entomophagous insects for pest control. As parasitic wasps often lay eggs on or into their associated hosts, parasitoids evolve to utilize several factors including venom, polydnavirus (PDV) to alter host physiology for successful parasitism. Some taxa of endoparasitoids produce teratocytes, which are a type of cell that is released into host insects when wasp eggs hatch. Teratocytes display multifunction in parasitism such as host nutritional exploration, immune and developmental regulation, by secreting plenty of proteins into host hemocoel. RESULTS A serpin (CvT‐serpin15) secreted by teratocytes was characterized. QPCR results showed the expressional level of CvT‐serpin15 was upregulated following bacterial challenges. Enzyme activity experiment indicated the recombinant CvT‐serpin15 protein could interfere with the growth of Gram‐positive bacteria Staphylococcus aureus . The survival rate assay demonstrated CvT‐serpin15 increased survival rate of Plutella xylostella infected by S. aureus . CONCLUSION CvT ‐ serpin15 secreted by teratocytes would boost the host immune system when pathogens invade host hemocoel during parasitism, and ultimately protect the development of wasp larva from bacterial infection. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 1526-498X , 1526-4998
    URL: Issue
    URL: Article
    DOI: 10.1002/ps.v77.10
    DOI: 10.1002/ps.6515
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2003455-6
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  • 5
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    Prediction of homoprotein and heteroprotein complexes by protein docking and template‐based modeling: A CASP‐CAPRI experiment
    Wiley ; 2016
    In:  Proteins: Structure, Function, and Bioinformatics Vol. 84, No. S1 ( 2016-09), p. 323-348
    Details
    In: Proteins: Structure, Function, and Bioinformatics, Wiley, Vol. 84, No. S1 ( 2016-09), p. 323-348
    Abstract: We present the results for CAPRI Round 30, the first joint CASP‐CAPRI experiment, which brought together experts from the protein structure prediction and protein–protein docking communities. The Round comprised 25 targets from amongst those submitted for the CASP11 prediction experiment of 2014. The targets included mostly homodimers, a few homotetramers, and two heterodimers, and comprised protein chains that could readily be modeled using templates from the Protein Data Bank. On average 24 CAPRI groups and 7 CASP groups submitted docking predictions for each target, and 12 CAPRI groups per target participated in the CAPRI scoring experiment. In total more than 9500 models were assessed against the 3D structures of the corresponding target complexes. Results show that the prediction of homodimer assemblies by homology modeling techniques and docking calculations is quite successful for targets featuring large enough subunit interfaces to represent stable associations. Targets with ambiguous or inaccurate oligomeric state assignments, often featuring crystal contact‐sized interfaces, represented a confounding factor. For those, a much poorer prediction performance was achieved, while nonetheless often providing helpful clues on the correct oligomeric state of the protein. The prediction performance was very poor for genuine tetrameric targets, where the inaccuracy of the homology‐built subunit models and the smaller pair‐wise interfaces severely limited the ability to derive the correct assembly mode. Our analysis also shows that docking procedures tend to perform better than standard homology modeling techniques and that highly accurate models of the protein components are not always required to identify their association modes with acceptable accuracy. Proteins 2016; 84(Suppl 1):323–348. © 2016 The Authors Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0887-3585 , 1097-0134
    URL: Issue
    URL: Article
    DOI: 10.1002/prot.v84.S1
    DOI: 10.1002/prot.25007
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1475032-6
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  • 6
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    Raman Spectroscopy in Combination with Background Near‐infrared Autofluorescence Enhances the In Vivo Assessment of Malignant Tissues
    Wiley ; 2005
    In:  Photochemistry and Photobiology Vol. 81, No. 5 ( 2005-09), p. 1219-1226
    Details
    In: Photochemistry and Photobiology, Wiley, Vol. 81, No. 5 ( 2005-09), p. 1219-1226
    Abstract: The diagnostic ability of optical spectroscopy techniques, including near‐infrared (NIR) Raman spectroscopy, NIR autofluorescence spectroscopy and the composite Raman and NIR autofluorescence spectroscopy, for in vivo detection of malignant tumors was evaluated in this study. A murine tumor model, in which BALB/c mice were implanted with Meth‐A fibrosarcoma cells into the subcutaneous region of the lower back, was used for this purpose. A rapid‐acquisition dispersive‐type NIR Raman system was employed for tissue Raman and NIR autofluorescence spectroscopic measurements at 785‐nm laser excitation. High‐quality in vivo NIR Raman spectra associated with an autofluorescence background from mouse skin and tumor tissue were acquired in 5 s. Multivariate statistical techniques, including principal component analysis (PCA) and linear discriminant analysis (LDA), were used to develop diagnostic algorithms for differentiating tumors from normal tissue based on their spectral features. Spectral classification of tumor tissue was tested using a leave‐one‐out, cross‐validation method, and the receiver operating characteristic (ROC) curves were used to further evaluate the performance of diagnostic algorithms derived. Thirty‐two in vivo Raman, NIR fluorescence and composite Raman and NIR fluorescence spectra were analyzed (16 normal, 16 tumors). Classification results obtained from cross‐validation of the LDA model based on the three spectral data sets showed diagnostic sensitivities of 81.3%, 93.8% and 93.8%; specificities of 100%, 87.5% and 100%; and overall diagnostic accuracies of 90.6%, 90.6% and 96.9% respectively, for tumor identification. ROC curves showed that the most effective diagnostic algorithms were from the composite Raman and NIR autofluorescence techniques.
    Type of Medium: Online Resource
    ISSN: 0031-8655 , 1751-1097
    URL: Article
    DOI: 10.1562/2005-02-24-RA-449
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 2048860-9
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  • 7
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    Electrochemical deposition of lithium coating on titanium implant with enhanced early stage osseointegration
    Wiley ; 2022
    In:  Journal of Biomedical Materials Research Part B: Applied Biomaterials Vol. 110, No. 11 ( 2022-11), p. 2399-2410
    Details
    In: Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, Vol. 110, No. 11 ( 2022-11), p. 2399-2410
    Abstract: Recently, a large number of studies have reported that lithium (Li) displayed a positive effect on osteogenesis. However, only a few studies have investigated the Li‐incorporated surfaces through electrochemical deposition. In this study, electrochemical deposition was conducted on a CHI600E electrochemical workstation. The characterization of electrochemical deposition (ECD) and ECD‐Li surfaces were detected by field‐emission scanning electron microscopy with energy‐dispersive spectrometer. rBMSCs were cultured on two surfaces for subsequent adhesion, proliferation and live/dead assay. To evaluate the effects of Li‐incorporated implants by electrochemical deposition on osseointegration in vivo, teeth extraction of two premolars and one first molar in bilateral mandible were performed on six male beagle dogs. After 3 months, ZDI and ZDI‐Li implants were inserted into the bilateral mandible of each beagle dog. Micro Computed Tomography (Micro‐CT) and hard tissue sectioning analysis were carried out to evaluate the osseointegration at 4‐ and 8‐weeks post‐implantation. Results showed that ECD‐Li surface promoted adhesion and proliferation of BMSCs in the early stage. More importantly, through micro‐CT analysis, the values of bone volume/total volume (BV/TV) (0.374 ± 0.015), bone‐implant contact (BIC) (0.831 ± 0.025), and Tb.Th (0.412 ± 0.007) in ZDI‐Li group was significantly higher than those of ZDI group (0.302 ± 0.009, 0.700 ± 0.023, 0.353 ± 0.001, p   〈  .01) at 4 weeks. Similarly, ZDI‐Li group manifested more bone contact with the implant surfaces at 4 weeks based on hard tissue sectioning analysis, whereas no significant difference was detected between two groups at 8 weeks. Therefore, incorporating Li into implant surface through ECD could enhance early osseointegration in vivo.
    Type of Medium: Online Resource
    ISSN: 1552-4973 , 1552-4981
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.b.v110.11
    DOI: 10.1002/jbm.b.35085
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2130917-6
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  • 8
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    Agrobacterium tumefaciens ferritins play an important role in full virulence through regulating iron homeostasis and oxidative stress survival
    Wiley ; 2020
    In:  Molecular Plant Pathology Vol. 21, No. 9 ( 2020-09), p. 1167-1178
    Details
    In: Molecular Plant Pathology, Wiley, Vol. 21, No. 9 ( 2020-09), p. 1167-1178
    Abstract: Ferritins are a large family of iron storage proteins, which are used by bacteria and other organisms to avoid iron toxicity and as a safe iron source in the cytosol. Agrobacterium tumefaciens , a phytopathogen, has two ferritin‐encoding genes: atu2771 and atu2477 . Atu2771 is annotated as a Bfr‐encoding gene (Bacterioferritin, Bfr) and atu2477 as a Dps‐encoding gene ( D NA binding p rotein from s tarved cells, Dps). Three deletion mutants (Δ bfr , Δ dps , and bfr ‐ dps double‐deletion mutant ΔbdF) of these two ferritin‐encoding genes were constructed to investigate the effects of ferritin deficiency on the iron homeostasis, oxidative stress resistance, and pathogenicity of A. tumefaciens . Deficiency of two ferritins affects the growth of A. tumefaciens under iron starvation and excess. When supplied with moderate iron, the growth of A. tumefaciens is not affected by the deficiency of ferritin. Deficiency of ferritin significantly reduces iron accumulation in the cells of A. tumefaciens , but the effect of Bfr deficiency on iron accumulation is severer than Dps deficiency and the double mutant ΔbdF has the least intracellular iron content. All three ferritin‐deficient mutants showed a decreased tolerance to 3 mM H 2 O 2 in comparison with the wild type. The tumour induced by each of three ferritin‐deficient mutants is less than that of the wild type. Complementation reversed the effects of ferritin deficiency on the growth, iron homeostasis, oxidative stress resistance, and tumorigenicity of A. tumefaciens . Therefore, ferritin plays an important role in the pathogenesis of A. tumefaciens through regulating iron homeostasis and oxidative stress survival.
    Type of Medium: Online Resource
    ISSN: 1464-6722 , 1364-3703
    URL: Article
    DOI: 10.1111/mpp.12969
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2020755-4
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  • 9
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    Blocking the ERK1/2 signal pathway can inhibit S100A12 induced human aortic smooth muscle cells damage
    Wiley ; 2017
    In:  Cell Biology International Vol. 41, No. 12 ( 2017-12), p. 1307-1315
    Details
    In: Cell Biology International, Wiley, Vol. 41, No. 12 ( 2017-12), p. 1307-1315
    Abstract: Increased levels of S100A12 and activated matrix metalloproteinase 2/9 (MMP‐2/9) produced by human aortic smooth muscle cells (HASMCs) have recently implicated in the development of thoracic aortic disease. In the present study, we investigated the effect of S100A12 on HASMCs and identified the intracellular signal pathways involved by Western blot. The results were shown that up‐expression of S100A12 in HASMCs induced cell apoptosis and inhibited cell proliferation. Additionally, S100A12 significantly increased the expression of MMP‐2, MMP‐9, and VCAM‐1 in HASMCs at translational levels. Furthermore, our results also showed that S100A12 induced HASMCs damage by increased related proteins expression was mediated by the activation of ERK1/2 signal pathway, whereas p38 MAPK had no effect on those processes. Blocked the activation of ERK1/2 could decrease S100A12 induced the apoptosis and inhibited cell proliferation of HASMCs. In conclusion, these results indicated that S100A12 could increase the expression of MMP‐2, MMP‐9, and vascular cell adhesion molecule 1 (VCAM‐1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. Therefore, antagonists of ERK1/2 may be useful for treating thoracic aortic dissection.
    Type of Medium: Online Resource
    ISSN: 1065-6995 , 1095-8355
    URL: Issue
    URL: Article
    DOI: 10.1002/cbin.v41.12
    DOI: 10.1002/cbin.10840
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1462519-2
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  • 10
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    Gö6983 attenuates breast cancer‐induced osteolysis by the apoptotic pathway
    Wiley ; 2020
    In:  Cell Biology International Vol. 44, No. 3 ( 2020-03), p. 838-847
    Details
    In: Cell Biology International, Wiley, Vol. 44, No. 3 ( 2020-03), p. 838-847
    Abstract: Bone metastasis caused by breast cancer leads to significant complications in treatment, and the resulting osteolysis considerably affects patients’ overall survival and quality of life. Gö6983 is a broad spectrum protein kinase C inhibitor. In this study, based on our finding that the Gö6983 inhibits osteolysis, we applied Gö6983 to the MDA‐MB‐231 breast cancer‐induced mouse bone metastasis model. And we found that Gö6983 has a strong inhibitory effect on the tumorigenic model of breast cancer by promoting the mitochondrial apoptosis pathway. Our study, therefore, demonstrates that Gö6983 has a potential inhibitory effect on breast cancer‐induced osteoclast activation and provides mechanistic insight that may prove useful for designing future treatments.
    Type of Medium: Online Resource
    ISSN: 1065-6995 , 1095-8355
    URL: Issue
    URL: Article
    DOI: 10.1002/cbin.v44.3
    DOI: 10.1002/cbin.11281
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1462519-2
    SSG: 12
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