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  • 1
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    Online Resource
    IL‐6 promotes metastasis of non‐small‐cell lung cancer by up‐regulating TIM‐4 via NF‐κB
    Wiley ; 2020
    In:  Cell Proliferation Vol. 53, No. 3 ( 2020-03)
    Details
    In: Cell Proliferation, Wiley, Vol. 53, No. 3 ( 2020-03)
    Abstract: Interleukin‐6 (IL‐6) is critical for the development of non‐small‐cell lung cancer (NSCLC). Recently, we identified T‐cell immunoglobulin domain and mucin domain 4 (TIM‐4) as a new pro‐growth player in NSCLC progression. However, the role of TIM‐4 in IL‐6‐promoted NSCLC migration, invasion and epithelial‐to‐mesenchymal transition (EMT) remains unclear. Materials and Methods Expressions of TIM‐4 and IL‐6 were both evaluated by immunohistochemical staining in NSCLC tissues. Real‐time quantitative PCR (qPCR), Western blot, flow cytometry and RT‐PCR were performed to detect TIM‐4 expression in NSCLC cells with IL‐6 stimulation. The roles of TIM‐4 in IL‐6 promoting migration and invasion of NSCLC were detected by transwell assay. EMT‐related markers were analysed by qPCR and Western blot in vitro, and metastasis was evaluated in BALB/c nude mice using lung cancer metastasis mouse model in vivo. Results High IL‐6 expression was identified as an independent predictive factor for TIM‐4 expression in NSCLC tissues. NSCLC patients with TIM‐4 and IL‐6 double high expression showed the worst prognosis. IL‐6 promoted TIM‐4 expression in NSCLC cells depending on NF‐κB signal pathway. Both TIM‐4 and IL‐6 promoted migration, invasion and EMT of NSCLC cells. Interestingly, TIM‐4 knockdown reversed the role of IL‐6 in NSCLC and IL‐6 promoted metastasis of NSCLC by up‐regulating TIM‐4 via NF‐κB. Conclusions TIM‐4 involves in IL‐6 promoted migration, invasion and EMT of NSCLC.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Issue
    URL: Article
    DOI: 10.1111/cpr.v53.3
    DOI: 10.1111/cpr.12776
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2019986-7
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  • 2
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    Mitogen‐activated protein kinase p38 and retinoblastoma protein signalling is required for DNA damage‐mediated formation of senescence‐associated heterochromatic foci in tumour cells
    Wiley ; 2013
    In:  The FEBS Journal Vol. 280, No. 18 ( 2013-09), p. 4625-4639
    Details
    In: The FEBS Journal, Wiley, Vol. 280, No. 18 ( 2013-09), p. 4625-4639
    Abstract: DNA ‐damaging agents are able to induce irreversible cell growth arrest and senescence in some types of tumour cells, thus contributing to the static feature of cancer. However, senescent tumour cells may re‐enter the cell cycle, leading to tumour relapse. Understanding the mechanisms that control the viability of senescent cells may be critical for tumour suppression. Primary human fibroblasts undergoing oncogene‐induced or replicative senescence are known to form senescence‐associated heterochromatin foci ( SAHF ), which contribute to the stability of the senescent state. However, it is unclear whether SAHF formation is universal in tumour cells. We report that the DNA ‐damaging agents doxorubicin and 7‐ethyl‐10‐hydroxycamptothecin were able to induce the formation of SAHF in some tumour cell types, and this induction was accompanied by activation of the retinoblastoma protein pathway. By contrast, tumour cells in which the retinoblastoma protein pathway could not be activated by doxorubicin or 7‐ethyl‐10‐hydroxycamptothecin failed to form SAHF . In parallel, tumour cells with deficient retinoblastoma protein were also unable to form SAHF . In addition, we show that the mitogen‐activated protein kinase p38 pathway was involved in tumour cell SAHF formation in response to doxorubicin and 7‐ethyl‐10‐hydroxycamptothecin. Furthermore, HMG box transcription factor 1 (HBP1), a downstream target of the mitogen‐activated protein kinase p38‐mediated senescence pathway, was required for SAHF formation. Taken together, the results of the present study highlight the roles of the mitogen‐activated protein kinase p38/retinoblastoma protein pathway in tumour cell SAHF formation in response to DNA ‐damaging agents, and provide new insights into the mechanisms of DNA damage‐mediated tumour suppression. Structured digital abstract HBP1 physically interacts with RB by anti bait coimmunoprecipitation (View Interaction: 1 , 2 )
    Type of Medium: Online Resource
    ISSN: 1742-464X , 1742-4658
    URL: Issue
    URL: Article
    DOI: 10.1111/febs.2013.280.issue-18
    DOI: 10.1111/febs.12435
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2172518-4
    SSG: 12
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  • 3
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    Effects of Ginsenoside Rg1 on Learning and Memory in a Reward‐directed Instrumental Conditioning Task in Chronic Restraint Stressed Rats
    Wiley ; 2017
    In:  Phytotherapy Research Vol. 31, No. 1 ( 2017-01), p. 81-89
    Details
    In: Phytotherapy Research, Wiley, Vol. 31, No. 1 ( 2017-01), p. 81-89
    Abstract: Ginsenoside Rg1 is one of the major active ingredients of Panax ginseng and has showed notable improving learning and memory effects in several behavioral tasks, such as water maze, shuttle‐box, and step‐through, based on avoidance. However, there was no report about the role of Rg1 on the performance of reward‐directed instrumental conditioning, which could reflect the adaptive capacity to ever‐changing environments. Thus, in this study, the reward devaluation test and conditional visual discrimination task were conducted to study the ameliorating effects of Rg1 on cognitive deficits, especially the loss of adaptation capacity in chronic restraint stress (CRS) rat model. Our results showed that rat subjected to CRS became insensitive to the changes in outcome value, and it significantly harmed the rat's performance in conditional visual discrimination task. Moreover, the levels of BDNF, TrkB, and Erk phosphorylation were decreased in the prefrontal cortex of CRS rats. However, these changes were effectively reversed by Rg1 (5 and 10 mg/kg, i.p.). Therefore, it demonstrated that Rg1 has a good ability to improve learning and memory and also ameliorate impaired adaptive capacity induced by CRS. This amelioration effect of Rg1 might be mediated partially by BDNF/TrkB/Erk pathway in prefrontal cortex. Copyright © 2016 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0951-418X , 1099-1573
    URL: Issue
    URL: Article
    DOI: 10.1002/ptr.v31.1
    DOI: 10.1002/ptr.5733
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1493490-5
    SSG: 15,3
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  • 4
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    Radioprotective effects of dammarane sapogenins against 60 Co‐induced myelosuppression in mice
    Wiley ; 2018
    In:  Phytotherapy Research Vol. 32, No. 4 ( 2018-04), p. 741-749
    Details
    In: Phytotherapy Research, Wiley, Vol. 32, No. 4 ( 2018-04), p. 741-749
    Abstract: Radiotherapy frequently induces failure of hematopoietic system and leads to myelosuppression. The objective of this study was to investigate the protective effect of dammarane sapogenins (DS), the hydrolysed product of the constituent ginsenosides of Panax ginseng , which are produced by gut metabolism, on radiation‐induced hematopoietic injury. Mice were exposed to 3.5 Gy 60 Co γ‐rays of total body radiation at a dose rate of 1.60 Gy per minute and treated with DS or granulocyte colony‐stimulating factor immediately after radiation. The general condition of the mice, the peripheral blood cell counts, multiple colony forming unit (CFU) assays of hematopoietic progenitor cells, hematopoietic stem cell counts, bone marrow histology, and spleen colony forming unit counts were then investigated. Our results indicated that administration with DS could ameliorate 60 Co‐irradiation induced damage and significantly increase the number of peripheral blood cells (white blood cells and platelets), 5 types of hematopoietic progenitor cells CFU (CFU‐GM, CFU‐E, BFU‐E, CFU‐Meg, and CFU‐GEMM), hematopoietic stem cell (Lin − c‐kit + Scal‐1 + ) numbers, and CFUs in the spleen, as well as improved bone marrow histopathology. All together, these results confirmed the enhancement of DS on hematopoiesis.
    Type of Medium: Online Resource
    ISSN: 0951-418X , 1099-1573
    URL: Issue
    URL: Article
    DOI: 10.1002/ptr.v32.4
    DOI: 10.1002/ptr.6027
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1493490-5
    SSG: 15,3
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  • 5
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    Pegylated interferon‐α‐2b combined with tenofovir disoproxil fumarate, granulocyte‐macrophage colony‐stimulating factor, and hepatitis B vaccine treatment for naïve HBeAg‐positive chronic hepatitis B patients: A prospective, multicenter, randomized controlled study
    Wiley ; 2022
    In:  Journal of Medical Virology Vol. 94, No. 11 ( 2022-11), p. 5475-5483
    Details
    In: Journal of Medical Virology, Wiley, Vol. 94, No. 11 ( 2022-11), p. 5475-5483
    Abstract: Hepatitis B surface antigen (HBsAg) loss or seroconversion is an ideal treatment endpoint for patients with chronic hepatitis B but is rarely achievable in  hepatitis B e‐antigen (HBeAg)‐positive patients using existing treatment strategies. In this study, the effect of pegylated interferon (peg‐IFN) alfa‐2b plus tenofovir disoproxil fumarate (TDF), granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), and hepatitis B vaccine was evaluated. This randomized controlled trial was conducted at nine liver centers in Chinese university hospitals from May 2018 to July 2020. Patients ( n  = 303) enrolled were randomly administered peg‐IFN‐α‐2b combined with TDF, GM‐CSF, and hepatitis B vaccine (experimental group); peg‐IFN‐α‐2b plus TDF (control group 2); or interferon‐α‐2b alone (control group 1). The primary efficacy endpoint was HBsAg seroconversion at 48 weeks and the secondary endpoint included safety. No differences in baseline HBsAg levels were observed among the groups. The primary endpoint was achieved in three (3.0%), one (1.03%), and one (1.19%) patient in the experimental group, control group 2, and control group 1, respectively. The incidence of HBsAg seroconversion at week 48 was not significantly different among the three groups ( p  = 0.629). However, the decrease in serum levels of HBsAg at week 48 was significantly higher in the experimental and control group 2 compared with that in control group 1 ( p  = 0.008 and 0.006, respectively). No significant difference between the experimental and control group 2 was observed ( p  = 0.619). Adverse events were not significantly different among the groups except for the lower incidence of neutropenia in the experimental group. Peg‐IFN‐α‐2b combined with TDF, GM‐CSF, and hepatitis B vaccine is not superior to peg‐IFN‐α‐2b combined with TDF in HBeAg‐positive naïve patients. Clinical Trials Registration: ChiCTR1800016173.
    Type of Medium: Online Resource
    ISSN: 0146-6615 , 1096-9071
    URL: Issue
    URL: Article
    DOI: 10.1002/jmv.v94.11
    DOI: 10.1002/jmv.28003
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 752392-0
    detail.hit.zdb_id: 1475090-9
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  • 6
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    Regulation of neuronal nitric oxide synthase exon 1f gene expression by nuclear factor-?B acetylation in human neuroblastoma cells
    Wiley ; 2007
    In:  Journal of Neurochemistry Vol. 101, No. 5 ( 2007-06), p. 1194-1204
    Details
    In: Journal of Neurochemistry, Wiley, Vol. 101, No. 5 ( 2007-06), p. 1194-1204
    Type of Medium: Online Resource
    ISSN: 0022-3042 , 1471-4159
    URL: Issue
    URL: Article
    DOI: 10.1111/jnc.2007.101.issue-5
    DOI: 10.1111/j.1471-4159.2006.04407.x
    Language: English
    Publisher: Wiley
    Publication Date: 2007
    detail.hit.zdb_id: 2020528-4
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  • 7
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    Vitamin D is related to handgrip strength in adult men aged 50 years and over: A population study from the TCLSIH cohort study
    Wiley ; 2019
    In:  Clinical Endocrinology Vol. 90, No. 5 ( 2019-05), p. 753-765
    Details
    In: Clinical Endocrinology, Wiley, Vol. 90, No. 5 ( 2019-05), p. 753-765
    Abstract: Handgrip strength (HGS) begins an accelerating decline around 50 years. Many of the studies performed in old adults have demonstrated a significant relationship between vitamin D and HGS, but the studies performed in participants with a broad age range have yielded conflicting results. The purpose of the study was to investigate the relationship between vitamin D and HGS using age 50 as a specific cut‐off. Design Population‐based, cross‐sectional study. Participants Totally 5102 participants (2911 males, 2191 females) from the TCLSIH Cohort. Measurements Serum concentration of 25‐hydroxyvitamin D (25(OH)D) was measured using an enzyme immunoassay. We divided participants into quartiles according to 25(OH)D, and the ranges for increasing quartiles were as follows: (males [≥50 years]: 10.94‐31.85, 31.88‐43.01, 43.20‐56.06, 56.20‐143.0; males [ 〈 50 years]: 11.11‐34.68, 34.71‐46.91, 46.96‐59.45, 59.50‐143.7; females [≥50 years] : 7.21‐30.01, 30.02‐40.18, 40.21‐52.44, 52.49‐275.4; females [ 〈 50 years]: 5.29‐28.91, 28.92‐40.19, 40.20‐51.90, 51.91‐140.2). HGS was measured with a hydraulic hand‐held dynamometer. Analysis of covariance was employed to explore the relationship. Results Among males aged above 50 years, the means (95% confidence interval) for HGS per body weight across the categories of serum 25(OH)D concentration were 0.523 (0.430‐0.638), 0.545 (0.447‐0.664), 0.543 (0.446‐0.661), 0.546 (0.449‐0.664) ( P trend   〈  0.01) after adjustment for potential confounding factors. However, no relationships were observed between serum 25(OH)D concentration and HGS in males aged below 50 years and females in the whole age range. Conclusions Serum 25(OH)D concentration was significantly related to HGS in males aged above 50 years, independent of confounding factors. Future studies are needed to clarify the age and sex relationship between serum 25(OH)D concentration and HGS.
    Type of Medium: Online Resource
    ISSN: 0300-0664 , 1365-2265
    URL: Issue
    URL: Article
    DOI: 10.1111/cen.2019.90.issue-5
    DOI: 10.1111/cen.13952
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2004597-9
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  • 8
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    Diagnostic Value of Salivary Real‐Time Quaking‐Induced Conversion in Parkinson's Disease and Multiple System Atrophy
    Wiley ; 2022
    In:  Movement Disorders Vol. 37, No. 5 ( 2022-05), p. 1059-1063
    Details
    In: Movement Disorders, Wiley, Vol. 37, No. 5 ( 2022-05), p. 1059-1063
    Abstract: Aggregation of α‐synuclein (oligomeric α‐syn) has been considered as the pathological hallmark of Parkinson's disease (PD) and multiple system atrophy (MSA). Studies showed oligomeric α‐syn/total α‐syn ratio was increased in the saliva of patients with PD, suggesting that seeding activity of salivary oligomeric α‐syn may be a novel biomarker for the diagnosis of PD and MSA. Objective This study aimed to evaluate the diagnostic value of salivary α‐syn seeding activity in patients with PD and MSA. Methods A total of 75 patients with PD, 18 patients with MSA, and 36 nonneurodegenerative healthy control subjects underwent salivary α‐syn real‐time quaking‐induced conversion (RT‐QuIC) assay. Results Salivary α‐syn RT‐QuIC assay distinguished patients with PD with 76.0% sensitivity (95% confidence interval [CI], 66.1–85.9) and 94.4% specificity (95% CI, 86.6–100.0). RT‐QuIC assay sensitivity reached 61.1% (95% CI, 36.2–86.1) in patients with MSA. No significant differences were observed in the diameter of salivary α‐syn fibrils examined by electron microscopy and in thioflavin T fluorescence intensity of salivary α‐syn fibrils detected by RT‐QuIC assay between patients with PD and MSA. Notably, the lag phase of RT‐QuIC assay from patients with PD was significantly shorter than that of patients with MSA, which might be clinically applicable to the discrimination between PD and MSA. Conclusions Salivary α‐syn seeding activity may serve as a novel biomarker for the clinical diagnosis of PD and MSA.© 2022 International Parkinson and Movement Disorder Society © 2022 International Parkinson and Movement Disorder Society
    Type of Medium: Online Resource
    ISSN: 0885-3185 , 1531-8257
    URL: Issue
    URL: Article
    DOI: 10.1002/mds.v37.5
    DOI: 10.1002/mds.28976
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2041249-6
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  • 9
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    Leakage‐Proof Electrolyte Chemistry for a High‐Performance Lithium–Sulfur Battery
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 30 ( 2021-07-19), p. 16623-16627
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 30 ( 2021-07-19), p. 16623-16627
    Abstract: Electrolyte leakage is a severe safety concern in lithium batteries. With highly volatile 1,2‐dimethoxyethane as solvent, the leakage related hazards are more pronounced in lithium‐sulfur (Li‐S) batteries. To address this concern, a leakage‐proof electrolyte is delicately designed through functionalizing the commercial electrolyte by Li 6 PS 5 Cl‐grafted poly(ethyl cyanoacrylate), which can interact readily with the aluminum‐plastic packing through hydrogen bond to immobilize the electrolyte. The moisture from ambient can also catalyze a further polymerization of the macromolecules to seal the leaking points and thereby to solve the leakage issue, endowing Li‐S batteries superior safety even in an artificial cut pouch cell. With a bare S loading of 4.9 mg cm −2 , the battery can deliver good endurance owing to the suppressed polysulfide shuttle by its polar groups. This work enlightens the design of leakage‐proof electrolyte and makes a milestone for high performance Li‐S batteries.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.30
    DOI: 10.1002/ange.202103209
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 10
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    Rapid and sensitive analysis of three polyphenols in tobacco by CE using homemade C 4 D with a mini detection cell
    Wiley ; 2012
    In:  ELECTROPHORESIS Vol. 33, No. 15 ( 2012-08), p. 2433-2440
    Details
    In: ELECTROPHORESIS, Wiley, Vol. 33, No. 15 ( 2012-08), p. 2433-2440
    Abstract: A rapid, sensitive, and practical CE with C 4 D detection was developed for the analysis of three polyphenols (rutin, scopoletin, and chlorogenic acid) in tobacco samples. The constructed mini detection cell (12 mm × 10 mm × 10 mm) of C 4 D featured with small inner cell volume (∼2 nL), smaller noise ( 〈 0.9 mV), repeatability, high strength and durableness. Three polyphenols were ultrasonically extracted with methanol–water (70:30, v/v) solution following SPE cleanup. The CE method was optimized with the running buffer of 150 mmol L −1 2‐amino‐2‐methyl‐1‐propanol (pH 11.2), and the applied separation voltage of +20 kV over a capillary of 50 μm id × 375 μm od × 50 cm (38 cm to the C 4 D window, 41.5 cm to the UV detector window), which gave a baseline separation of three polyphenols within ca . 6 min. The method provided the limits of quantification (S/N = 10) at about 0.08–0.15 μg g −1 for three polyphenols, whereas the overall recoveries ranged from 82% to 88%. The proposed method has been successfully applied to measure three polyphenols in the actual tobacco samples, and their contents were calculated and evaluated.
    Type of Medium: Online Resource
    ISSN: 0173-0835 , 1522-2683
    URL: Issue
    URL: Article
    DOI: 10.1002/elps.v33.15
    DOI: 10.1002/elps.201200057
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 1475486-1
    SSG: 12
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