In:
Development, The Company of Biologists, Vol. 129, No. 24 ( 2002-12-15), p. 5839-5846
Abstract:
The murine autosomal recessive juvenile cystic kidney (jck)mutation results in polycystic kidney disease. We have identified injck mice a mutation in Nek8, a novel and highly conserved member of the Nek kinase family. In vitro expression of mutated Nek8results in enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect in the Nek8 gene can cause cystic disease,we performed a cross-species analysis: injection of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the ortholog ofNek8 resulted in the formation of pronephric cysts. These results demonstrate that comparative analysis of gene function in different model systems represents a powerful means to annotate gene function.
Type of Medium:
Online Resource
ISSN:
1477-9129
,
0950-1991
Language:
English
Publisher:
The Company of Biologists
Publication Date:
2002
detail.hit.zdb_id:
2007916-3
SSG:
12
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