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  • SAGE Publications  (3)
  • Biodiversity Research  (3)
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  • SAGE Publications  (3)
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  • 1
    Online Resource
    Online Resource
    SAGE Publications ; 2023
    In:  Pharmacognosy Magazine Vol. 19, No. 3 ( 2023-09), p. 530-538
    In: Pharmacognosy Magazine, SAGE Publications, Vol. 19, No. 3 ( 2023-09), p. 530-538
    Abstract: Metabolic syndrome is an assortment of conditions that often happens together and upsurge your risk of diabetes, stroke, and heart disease. One strategy for the prophylaxis and treatment of diabetes and obesity is to inhibit the enzymes activities, which include α-glucosidase, α-amylase, and pancreatic lipase. Objectives To screen the effect of 16 Chinese medicinal herbs on inhibition of α-glucosidase, α-amylase, and pancreatic lipase. Materials and Methods The water extraction of 16 traditional Chinese herbal medicines was used to estimate activity in vitro from different families against α-glucosidase, α-amylase, and pancreatic lipase by using spectrophotometry with p-nitrophenyl-α-D-glucopyranoside (PNPG), starch derivatives, and 4-methylumbelliferone (4-MUO), respectively, as a substrate. Results The results showed that the water extraction yield of the 16 rhizomes ranged from 0.2% to 4.0%. Among the tested extracts, the extract from Gastrodia elata exhibited the strongest effect on α-amylase (The half-maximal inhibitory concentration (IC 50 )) = 4.10 mg/mL). The extracts from Pinellia ternate and Arisaema heterophyllum exhibited noteworthy effects on α-glucosidase (IC 50 = 29.21 and 41.13 µg/mL, respectively), and Corydalis turtschaninovii possessed the highest effect on pancreatic lipase (IC 50 = 4.11 µg/mL) and a stronger effect than orlistat (IC 50 = 4.52 µg/mL). Conclusion This study provides a basis for screenings and in-depth studies of anti-obesity and anti-diabetes drugs. Further isolation, identification, and characterization of active compounds should be carried out in the future.
    Type of Medium: Online Resource
    ISSN: 0973-1296 , 0976-4062
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2274976-7
    SSG: 15,3
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  • 2
    In: Pharmacognosy Magazine, SAGE Publications
    Abstract: Scutellarin, which is obtained from Erigeron breviscapus Hand-Mazz (EBHM), is a flavonoid that has demonstrated the ability to safeguard neural stem cells from hypoxia-induced damage and prevent cell apoptosis. The aim of this study was to investigate the beneficial impacts of Scutellarin on mitophagy in both in vivo and in vitro models of myocardial ischemia-reperfusion (I/R) injury. Materials and Methods Prior to inducing models of myocardial I/R injury, mice were administered either Scutellarin (50 mg/kg/day) or a vehicle for seven consecutive days. The mice underwent I/R injury (30 min of left anterior descending (LAD) coronary artery ligation followed by 120 min of reperfusion). Myocardial injury markers were assessed by the enzyme-linked immunosorbent assay (ELISA). The size of the myocardial infarction was measured via 2,3,5-triphenyl tetrazolium chloride triazole staining, and the protein expression of LC3 and caspase-3 was determined through Western blot analysis. In vitro experiments were conducted utilizing cultured H9C2 cells subjected to an oxygen-glucose deprivation/reoxygenation model to investigate the underlying mechanism(s) of Scutellarin’s positive effects (50 µM). Results It shows that Scutellarin treatment reduced the size of the myocardial infarctions and decreased the levels of myocardial injury markers. Western blot analysis showed that protein expression of caspase-3 was decreasing and the ratio of LC3Ⅱ to LC3Ⅰ was increasing in the Scutellarin group. In vitro, Scutellarin decreased oxidative stress markers, stabilized mitochondrial membrane potential (∇Ψm), decreased mitochondrial permeability transition pore (mPTP) opening rate, promoted mitochondrial fusion, inhibited mitochondrial fission, and increased adenosine triphosphate (ATP) production and cell viability. Scutellarin increased the commitment of mitophagy by regulating Pink and Parkin, while apoptosis decreased. cAMP-response element-binding protein (CREB) and extracellular signal-regulated kinases 1 and 2 (ERK1/2) phosphorylation were also modulated by Scutellarin. Conclusion The myocardial protective effect of Scutellarin may be associated with the phosphorylation of CREB and ERK1/2.
    Type of Medium: Online Resource
    ISSN: 0973-1296 , 0976-4062
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2274976-7
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
  • 3
    In: Pharmacognosy Magazine, SAGE Publications
    Abstract: Psoriasis vulgaris is an immune-mediated inflammatory skin disease that is associated with depression. In this study, we investigated the effect of Xiegan–Liangxue–Jiedu (XGLXJD) decoction, a traditional Chinese medicine formula, on psoriasis based on network pharmacology, molecular docking, and animal experiments. Materials and Methods A protein–protein interaction (PPI) network was constructed using the overlapping targets of XGLXJD decoction and psoriasis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using Metascape database. High-performance liquid chromatography (HPLC) was used to investigate the main compounds of XGLXJD decoction. Molecular docking was performed to predict the potential interaction between the main compounds and proteins of interest. A C57 mouse model of psoriasis was established via continuous exposure to imiquimod. Seven days later, the XGLXJD decoction was orally administered at increasing doses for 6 days. The psoriasis area and severity index were calculated. Hematoxylin and eosin staining was used to examine skin morphology. Enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of adenylyl cyclase (AC), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), interleukin-17 (IL-17), and tumor necrosis factor-α (TNF-α). Sucrose preference test and forced swimming test were used to assess depression-like behavior. Immunohistochemical (IHC) staining and immunofluorescence were used to investigate the cAMP-response element binding protein (CREB) signaling pathway. Results Overall, 162 overlapping targets were generated. A total of 398 biological processes, 84 molecular functions, and 47 cellular components were identified via GO analysis, whereas 140 pathways were identified via KEGG pathway analysis. The most notable signaling pathways were cAMP as well as downstream IL-17 and TNF-α signaling pathways. HPLC analysis revealed that the main compounds of XGLXJD decoction were paeoniflorin, isorhamnetin, quercetin, luteolin, kaempferol, and baicalein. The molecular docking assay indicated that the docking energies of the main compounds of XGLXJD decoction to the top hub genes were less than −5 kcal/mol. ELISA revealed that the administration of XGLXJD decoction decreased the levels of pro-inflammatory cytokines (TNF-α and IL-17). Furthermore, the AC, cAMP, and PKA levels were enhanced after its administration. IHC staining demonstrated that the administration of XGLXJD decoction activated the AC–cAMP–PKA–CREB signaling pathway in skin. In addition, it enhanced sucrose preference and forced swimming time percentage. It also enhanced the expression of cAMP and PKA in the hippocampus. Conclusion XGLXJD decoction alleviated psoriasis and depression-like behavior. The AC–cAMP–PKA–CREB signaling pathway may play a crucial role in mediating this effect.
    Type of Medium: Online Resource
    ISSN: 0973-1296 , 0976-4062
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2274976-7
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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