In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2021-5-25), p. e1009587-
Abstract:
Human pluripotent stem cells (PSCs) express human endogenous retrovirus type-H (HERV-H), which exists as more than a thousand copies on the human genome and frequently produces chimeric transcripts as long-non-coding RNAs (lncRNAs) fused with downstream neighbor genes. Previous studies showed that HERV-H expression is required for the maintenance of PSC identity, and aberrant HERV-H expression attenuates neural differentiation potentials, however, little is known about the actual of function of HERV-H. In this study, we focused on ESRG, which is known as a PSC-related HERV-H-driven lncRNA. The global transcriptome data of various tissues and cell lines and quantitative expression analysis of PSCs showed that ESRG expression is much higher than other HERV-Hs and tightly silenced after differentiation. However, the loss of function by the complete excision of the entire ESRG gene body using a CRISPR/Cas9 platform revealed that ESRG is dispensable for the maintenance of the primed and naïve pluripotent states. The loss of ESRG hardly affected the global gene expression of PSCs or the differentiation potential toward trilineage. Differentiated cells derived from ESRG-deficient PSCs retained the potential to be reprogrammed into induced PSCs (iPSCs) by the forced expression of OCT3/4, SOX2, and KLF4. In conclusion, ESRG is dispensable for the maintenance and recapturing of human pluripotency.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1009587
DOI:
10.1371/journal.pgen.1009587.g001
DOI:
10.1371/journal.pgen.1009587.g002
DOI:
10.1371/journal.pgen.1009587.g003
DOI:
10.1371/journal.pgen.1009587.g004
DOI:
10.1371/journal.pgen.1009587.g005
DOI:
10.1371/journal.pgen.1009587.s001
DOI:
10.1371/journal.pgen.1009587.s002
DOI:
10.1371/journal.pgen.1009587.s003
DOI:
10.1371/journal.pgen.1009587.s004
DOI:
10.1371/journal.pgen.1009587.s005
DOI:
10.1371/journal.pgen.1009587.s006
DOI:
10.1371/journal.pgen.1009587.s007
DOI:
10.1371/journal.pgen.1009587.s008
DOI:
10.1371/journal.pgen.1009587.s009
DOI:
10.1371/journal.pgen.1009587.s010
DOI:
10.1371/journal.pgen.1009587.s011
DOI:
10.1371/journal.pgen.1009587.s012
DOI:
10.1371/journal.pgen.1009587.s013
DOI:
10.1371/journal.pgen.1009587.s014
DOI:
10.1371/journal.pgen.1009587.r001
DOI:
10.1371/journal.pgen.1009587.r002
DOI:
10.1371/journal.pgen.1009587.r003
DOI:
10.1371/journal.pgen.1009587.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2186725-2
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