In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 12 ( 2022-12-2), p. e1010518-
Abstract:
Cancer progression is associated with the evolutionary accumulation of genetic mutations that are biologically significant. Mutations of the androgen receptor (AR) are associated with the development of prostate cancer (PCa) by responding to non-androgenic hormones, and the lack of annotations in their responsiveness to hormone ligands remains a daunting challenge. Here, we have used a yeast reporter system to quickly evaluate the responsiveness of all fifty clinical AR mutations to a variety of steroidal ligands including dihydrotestosterone (DHT), 17β-estradiol (E2), progesterone (PROG), and cyproterone acetate (CPA). Based on an AR-driven reporter that synthesizes histidine, a basic amino acid required for yeast survival and propagation, the yeast reporter system enabling clonal selection was further empowered by combining with a random DNA mutagenesis library to simulate the natural evolution of AR gene under the selective pressures of steroidal ligands. In a time-frame of 1–2 weeks, 19 AR mutants were identified, in which 11 AR mutants were validated for activation by tested steroidal compounds. The high efficiency of our artificial evolution strategy was further evidenced by a sequential selection that enabled the discovery of multipoint AR mutations and evolution directions under the pressure of steroidal ligands. In summary, our designer yeast is a portable reporter module that can be readily adapted to streamline high-throughput AR-compound screening, used as a PCa clinical reference, and combined with additional bioassay systems to further extend its potential.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010518
DOI:
10.1371/journal.pgen.1010518.g001
DOI:
10.1371/journal.pgen.1010518.g002
DOI:
10.1371/journal.pgen.1010518.g003
DOI:
10.1371/journal.pgen.1010518.g004
DOI:
10.1371/journal.pgen.1010518.g005
DOI:
10.1371/journal.pgen.1010518.t001
DOI:
10.1371/journal.pgen.1010518.t002
DOI:
10.1371/journal.pgen.1010518.t003
DOI:
10.1371/journal.pgen.1010518.s001
DOI:
10.1371/journal.pgen.1010518.s002
DOI:
10.1371/journal.pgen.1010518.s003
DOI:
10.1371/journal.pgen.1010518.s004
DOI:
10.1371/journal.pgen.1010518.s005
DOI:
10.1371/journal.pgen.1010518.s006
DOI:
10.1371/journal.pgen.1010518.s007
DOI:
10.1371/journal.pgen.1010518.s008
DOI:
10.1371/journal.pgen.1010518.s009
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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